Room-Temperature Luminescence of Formaldehyde-Condensed Amines Adsorbed on Filter Paper

Solid Surface Luminescence: A Study of the Glyoxylic Acid and Formaldehyde/Glutaraldehyde Histofluorescence Methods

“…This is a marked departure from amines derivatized by the ALFA method, which requires a solid surface for the reaction to take place (3). As shown in Table 3, the intensity of the adsorbed GA-derivatized amines was also usually higher than those reacted with GA in solutions.…”

“…This can be attributed to fluorescence quenching. A previous study had identified that phosphorescence or a long lived fluorescence was the source of luminescence for ALFA derivatized amines (3). Because the signal intensities are so much lower when the instrument is in the phosphorescence mode, it can be concluded that amines reacted with FAGLU and GA have a shorter luminescence lifetimes that those derivatized using the ALFA method.…”

“…2 Current address: 1 Cargill Dr., Eddyville, Iowa 52553. 3 Current address: Department of Chemistry, the University of Nebraska, Lincoln, Nebraska 68501. paper was used as the solid substrate. Samples were mounted on a card using poster putty, which was placed in the solid sample holder, or were placed in the instrument's powder sample holder.…”

“…Recently, we reported the luminescence of amines which were adsorbed on filter paper and condensed by the aluminum-formaldehyde (ALFA) method (3). It was concluded that amines adsorbed on filter paper react similarly to those found in tissues, making filter paper a potential surface for the modeling of histochemical reactions.…”