Root canal content from primary endodontic infection and upregulation of gelatinases in fibroblast cells

Abstract

Root canal contents from primary endodontic infections had gelatinolytic activity for MMP-2. Moreover, CMP was effective in reducing endotoxin levels and their antigenicity against fibroblasts on gelatinolytic activity.

Clinical efficacy of EDTA ultrasonic activation in the reduction of endotoxins and cultivable bacteria

“…Although the threshold 1.2 9 10 7 (2 9 10 3 -4 9 10 7 ) a 1.5 9 10 4 (2.2 9 10 3 -3 9 10 4 ) b 2.4 9 10 3 (1.9 9 10 2 -4.3 9 10 3 ) b 8.4 9 10 3 (2.3 9 10 2 -1.1 9 10 4 ) b * Different letters indicate statistical significance for endotoxin or bacteria between the different clinical times (P < 0.05). levels of endotoxin required in the root canals to damage the periapical tissues still have to be determined, it has long been known that minimal amounts of endotoxins are capable of inducing or maintaining an inflammatory process (Wilson et al 1996, Xavier et al 2013, Herrera et al 2015. The residual endotoxins found in the present study after CMP, if allowed access to the periradicular tissues after root filling, would modulate host immune responses by expression of cytokines and MMPs (Herrera et al 2015).…”

“…, Herrera et al . ). The residual endotoxins found in the present study after CMP, if allowed access to the periradicular tissues after root filling, would modulate host immune responses by expression of cytokines and MMPs (Herrera et al .…”

“…The residual endotoxins found in the present study after CMP, if allowed access to the periradicular tissues after root filling, would modulate host immune responses by expression of cytokines and MMPs (Herrera et al . ). Thus, every subsequent procedure performed during root canal treatment to obtain endotoxin‐free root canals should be considered.…”

“…levels of endotoxin required in the root canals to damage the periapical tissues still have to be determined, it has long been known that minimal amounts of endotoxins are capable of inducing or maintaining an inflammatory process (Wilson et al 1996, Xavier et al 2013, Herrera et al 2015. The residual endotoxins found in the present study after CMP, if allowed access to the periradicular tissues after root filling, would modulate host immune responses by expression of cytokines and MMPs (Herrera et al 2015). Thus, every subsequent procedure performed during root canal treatment to obtain endotoxin-free root canals should be considered.…”

Effect of ultrasonic activation on the reduction of bacteria and endotoxins in root canals: a randomized clinical trial

“…, Herrera et al . , ). Therefore, alternative approaches have been proposed to supplement the antimicrobial effect of chemo‐mechanical preparation in infected root canals, including a final irrigation protocol using additional ultrasonic activation of the irrigant (Siqueira & Rôças ).…”