Root nodules of some tropical legumes in Singapore

Lim, Gina; Ng, H. L.

doi:10.1007/bf00010088

Cited by 18 publications

(3 citation statements)

References 7 publications

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“…Nodulation in this sub-family is uncommon and concentrated in a few genera, such as Chamaecrista, Dimorphandra and (de Faria et al 1989;Sprent 1995;Sprent 2001), all of which do not occur in Hong Kong. A. microsperma was non-nodulating in Hong Kong, which was also considered non-nodulating generally (Allen and Allen 1981;Franco and de Faria 1997) except the skeptical studies in Singapore (Lim and Ng 1977) and Pakistan (Athar and Mahmood 1980). The nodulation status of Bowringia callicarpa has to be *New record of nodulation report further investigated as it was examined in one location only and inoculation test was not performed.…”

Section: Discussionmentioning

confidence: 96%

Nodulation of native woody legumes in Hong Kong, China

Hau

2008

Plant Soil

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Woody legumes can play an important role in forest restoration on degraded land but the knowledge of woody legumes has lagged behind their uses. This study is a pioneer investigation to explore the ability of native woody legumes to form root nodules and fix nitrogen in Hong Kong. Nine sites of different habitat types were surveyed during both wet and dry seasons for two years. Young plants of woody legumes along studied transects were excavated. The patterns of nodulation and nodule morphology were recorded and the nitrogen fixing ability was tested by acetylene-reduction-assay. Twentyeight species in 16 genera were examined, of which 20 species were nodulating and eight non-nodulating, including all six species in the Caesalpinioideae. Five species were new records to the world's nodulation inventory. Bowringia callicarpa was a new species and genus examined, which was non-nodulating. The overall nodulation pattern was consistent with previous studies. Nodulation was more profuse in some shrub species while inconsistent in most tree species. Species with higher proportion of nodulated individual plants also tended to have more nodules in each plant.Spherical nodules were common in shrub and woody climber species whilst tree species usually had woody indeterminate nodules. Seasonal difference in the amount of senescent nodules was noted in most species. All the nodules tested by acetylene-reductionassay were effectively nitrogen-fixing, with nitrogenase activity ranging from 4 μmol C 2 H 4 g −1 h −1 to 20 μmol C 2 H 4 g −1 h −1 , which was comparable to other tropical tree species. The findings in nodulation pattern and nitrogen fixing ability of these species are essential in their application in forest restoration on degraded lands.

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Section: Discussionmentioning

confidence: 96%

Nodulation of native woody legumes in Hong Kong, China

Hau

2008

Plant Soil

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“…In particular, Rhizobium and Mesorhizobium genomic species were never found to nodulate exclusively either a particular host or a particular plant. This co-occurrence of slow-and fast-growing rhizobia on the same host genus or species appears to happen quite commonly (24,43,49,54). Even when several legume species occurred at a site, in most cases, all of the species were predominantly nodulated with the commonest rhizobial species found at that site.…”

Section: Discussionmentioning

confidence: 99%

Molecular Diversity of Rhizobia Occurring on Native Shrubby Legumes in Southeastern Australia

Lafay

Burdon

1998

Appl Environ Microbiol

128

103

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The structure of rhizobial communities nodulating native shrubby legumes in open eucalypt forest of southeastern Australia was investigated by a molecular approach. Twenty-one genomic species were characterized by small-subunit ribosomal DNA PCR-restriction fragment length polymorphism and phylogenetic analyses, among 745 rhizobial strains isolated from nodules sampled on 32 different legume host species at 12 sites. Among these rhizobial genomic species, 16 belonged to the Bradyrhizobium subgroup, 2 to theRhizobium leguminosarum subgroup, and 3 to the Mesorhizobium subgroup. Only one genomic species corresponded to a known species (Rhizobium tropici). The distribution of the various genomic species was highly unbalanced among the 745 isolates, legume hosts, and sites.Bradyrhizobium species were by far the most abundant, andRhizobium tropici dominated among the Rhizobiumand Mesorhizobium isolates in the generally acid soils where nodules were collected. Although a statistically significant association occurred between the eight most common genomic species and the 32 hosts, there was sufficient overlap in distributions that no clear specificity between rhizobial genomic species and legume taxa was observed. However, for three legume species, some preference for particular genomic species was suggested. Similarly, no geographical partitioning was found.

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“…One of these, strain NGR234 (= MPIK3030), has been used for studying the genetics of legume-Rhizobium associations (4). Since other, similar, rhizobia have been isolated from a wide range of legumes (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18), it seemed that this class of rhizobia might prove useful in studying the genetics of nodulation of legumes normally associated with Bradyrhizobium species. A preliminary account of this work has been presented (19).…”

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confidence: 99%

Plasmid-linked nif and “ nod ” genes in fast-growing rhizobia that nodulate Glycine max, Psophocarpus tetragonolobus , and Vigna unguiculata

Broughton

Heycke²,

Pankhurst³

1984

Proc. Natl. Acad. Sci. U.S.A.

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Forty-nine fast-growing Rhizobium strains from the nodules of 26 different tropical legume genera were screened to find isolates that would (z) nodulate, e.g., winged beans, so producing large nodules for RNA and protein isolation; (ii) also nodulate various small-seeded legumes, thus allowing screening of large numbers of mutants; and (iii) harbor plasmids containing nif structural genes as well as other functions involved in nodulation. On the basis of six different criteria, this rhizobial group appeared intermediate between classical fast-and slow-growing organisms, yet all contained plasmids. Plasmid numbers varied from one to five. Hybridizations between DNA prepared from nifDH and the putatative "nod" region of R. melioi and these plasmids bound to nitrocellulose filters suggested that nif-nod genes are linked on a single sym plasmid. A broad-host-range strain containing a single sym plasmid was chosen for further study. Its plasmid, pMPIK3030a, was isolated on cesium chloride gradients and cloned in the cosmid pJB8, and the overlapping fragments were mapped by homology with the nif and nod regions of R. meliloli. As the wild-type plasmid pMPIK3030a was not selftransmissible, confirmation that the nod genes detected by homology were responsible for nodulation was obtained by introducing the mobilization functions of RP4 (together with TnS) and selecting transconjugants resistant to kanamycin and neomycin. Transconjugants (obtained at a frequency of about 10-6 per recipient) in Agrobacterium tumefaciens cured of the Ti plasmid produced ineffective nodules on Vigna unguiculata, those in nonnodulating (Nod-) R. melioti were partially effective, while those in Nod-R. leguminosarum were often fully effective.Many tropical and some temperate legumes are nodulated by slow-growing rhizobia. Recognized Rhizobium species include R. japonicum and R. lupini, while the large number of unnamed strains that nodulate Vigna and related hosts are referred to as the "cowpea" miscellany. Recently, a suggestion has been made to group these strains together with R. japonicum and R. lupini into a new genus of the Rhizobiaceae: Bradyrhizobium (1).Normal hosts of Bradyrhizobium species include many of the most important grain legumes of the world (e.g., Arachis hypogaea, Cicer arietinum, Glycine max, Psophocarpus tetragonolobus, Vigna radiata, and Vigna unguiculata) as well as most other tropical cover and pasture plants. Unfortunately, Bradyrhizobium species have proven refractive to molecular-genetic analysis of the sort used with fast-growing rhizobia, primarily because of difficulties associated with detection of sym plasmids (2).Fast-growing rhizobia exist, however, that are capable of forming effective nodules with many of the hosts normally associated with Bradyrhizobium species (3). One of these, strain NGR234 (= MPIK3030), has been used for studying the genetics of legume-Rhizobium associations (4). Since other, similar, rhizobia have been isolated from a wide range of legumes (5-18), it seemed that this clas...

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Root nodules of some tropical legumes in Singapore

Cited by 18 publications

References 7 publications

Nodulation of native woody legumes in Hong Kong, China

Nodulation of native woody legumes in Hong Kong, China

Molecular Diversity of Rhizobia Occurring on Native Shrubby Legumes in Southeastern Australia

Plasmid-linked nif and “ nod ” genes in fast-growing rhizobia that nodulate Glycine max, Psophocarpus tetragonolobus , and Vigna unguiculata

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