Root production and plantlet development in tissue-cultured conifers

Mohammed, Gina H.; Vidaver, William

doi:10.1007/bf00043405

Cited by 62 publications

(30 citation statements)

References 89 publications

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“…It is generally considered that the rooting and acclimatization stages of microcuttings are the most critical steps in conifer micropropagation (Franclet et al, 1980;Rancillac et al, 1982;Mohammed and Vidaver, 1988 (Scaltsoyiannes, 1988). A large variation among clones was also observed by Aitken-Christie and Thorpe (1984) who worked on rooting of P radiata microcuttings and also by Kleinschmidt and Schmidt (1977) and Zobel and Talbert (1987) …”

Section: Rooting Of Shootsmentioning

confidence: 99%

“…Thus, a reproducible and efficient procedure for mass propagation of outstanding genotypes by in vitro culture techniques was investigated as a potential alternative. Although progress in micropropagation of conifers through organogenesis from organ explants has been achieved, rooting of the micropropagated shoots (microcuttings) and the acclimatization of plantlets are still a problem (Jelaska, 1987;Mohammed and Vidaver, 1988;Stiff et al, 1989). According to many workers, further research on the influence of factors such as donor age, genotype, type of explant, microcutting quality, auxin treatment, root system and environmental conditions, on rooting and acclimatization is required (Franclet et al, 1980;Rancillac et al, 1982;Mohammed and Vidaver, 1988).…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of the pine hybrid Pinus brutia (Ten) × Pinus halepensis (Mill) by culturing fascicle shoots

et al. 1994

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Summary — Fascicle shoots proved to be an ideal plant material for micropropagation of the pine hybrid P brutia (Ten) x P halepensis (Mill). This could be possibly attributed to their morphological and physiological state. Induced fascicle shoots of 4-yr-old seedlings were used as explants. Their induction was achieved by spraying once with the new herbicide Arsenal (1 000 mg I -1 ). First, explants were elongated in vitro on LP medium and then transferred to the multiplication stage. Multiplication was accomplished by decapitating, quick-dipping in 0.22 mM BA and inoculating the explants on BIMI medium. On the induced first-generation shoots the same procedure was applied, in order to obtain second-generation shoots and then these were proceeded to the rooting and acclimatization stages. In particular, when microcuttings were pretreated with 2.46 μM IBA + 2.7 μM NAA + 0.65% agar w/v + 1.5% sucrose w/v for 7 d and then transferred to greenhouse conditions, a good root system was developed within an 8-12-week period. A large variation in rootability was noted between clones. The above method may be proved to be efficient for clones that exhibit high rooting ability.

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Section: Rooting Of Shootsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Micropropagation of the pine hybrid Pinus brutia (Ten) × Pinus halepensis (Mill) by culturing fascicle shoots

et al. 1994

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“…Since the extensive articles by Gaspar and Coumans (1987) and Mohammed and Vidaver (1988) appeared over 20 years ago, no other review on root production and plantlet development in conifers has been published. Some aspects, widely discussed in those reviews, such as biological factors, root morphogenesis, genetic stability and acclimatization, are not covered in the present review.…”

Section: Introductionmentioning

confidence: 99%

Adventitious rooting of conifers: influence of physical and chemical factors

Ragonezi

Klimaszewska

Castro

et al. 2010

Trees

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In conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from controlled seed orchard pollinations. This can be achieved, in some species, either through rooted cuttings or rooted microshoots, the latter regenerated through tissue culture in vitro. Thus far, both strategies have been used but often achieved limited success mainly because of difficult and inefficient rooting process. In this overview of technology, we focus on the progress in defining the physical and chemical factors that help the conifer cuttings and microshoots to develop adventitious roots. These factors include plant growth regulators, carbohydrates, light quality, temperature and rooting substrates/media as major variables for development of reliable adventitious rooting protocols for different conifer species.

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“…Other possible explanation of these observations could be finding in Mohammed and Vidaver (1988), they speculate that light and temperature affect rooting by controlling auxin and so dark and low temperature would have a promoting effect as happened in this work. Also, less cell wall deposition, less vascular tissue and thinner walls are expected in dark conditions, that could facilitate the movements of exogenous plant growth regulator to explant regeneration sites (Hartmann et al, 1997).…”

Section: Experiments 3: Different Carbon Source and Temperaturementioning

confidence: 91%

Effects of Carbon Source, Carbon Concentration and Culture Conditions on in Vitro Rooting of Pinus Pinea L. Microshoots

Zavattieri¹,

Lima²,

Sobral³

et al. 2009

Acta Hortic.

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Clonal propagation via adventitious bud stimulation from cotyledon explants has been reported before in stone pine (Pinus pinea L.), however the number of rooted shoots had low frequency and short growth span. Therefore the main objective of the present work was to increase the number and quality of roots per shoot in order to ensure the survival and growth of a great number of plants in the acclimation phase. To achieve this, different combinations of carbon source (sucrose or glucose) at different concentrations, under different environmental conditions (temperature and light), were tested in the induction and expression phases of the adventitious root formation. Shoots of different clones obtained via organogenesis have been employed for the experiments. Observations were made on the rooting percentage, root length and number of roots per shoot. The results showed a general increased of the number of roots per shoot and an earlier root formation when glucose was used as a carbon source. However, there were no differences in the percentage of rooting between the carbons sources tested. The best results were obtained using 0.117 M of glucose and dark treatment combined with 19 ºC during the induction phase of the rhizogenic process. Light and low sugar concentration proved to be beneficial for the expression phase, increasing the root length. A remarkable interclonal difference in the ability to form roots was observed. It was possible to obtain a rooting percentage of more than 75% in several of the tested clones.

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Root production and plantlet development in tissue-cultured conifers

Cited by 62 publications

References 89 publications

Micropropagation of the pine hybrid Pinus brutia (Ten) × Pinus halepensis (Mill) by culturing fascicle shoots

Micropropagation of the pine hybrid Pinus brutia (Ten) × Pinus halepensis (Mill) by culturing fascicle shoots

Adventitious rooting of conifers: influence of physical and chemical factors

Effects of Carbon Source, Carbon Concentration and Culture Conditions on in Vitro Rooting of Pinus Pinea L. Microshoots

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