Rooting micropropagated shoots from mature radiata pine

Abstract

A reliable method of rooting shoots from mature micropropagated Pinusradiata D. Don has been developed. At the beginning of the research, difficulty in achieving consistent results was experienced. At the end of the series of described experiments, high rooting percentages (78%) were attained. The condition of shoots is important. Shoots that had been cold-stored prior to rooting did not survive. Best rooting was obtained in a free-draining peat–pumice–perlite medium in propagation trays maintained under controlled environmental conditions. A well-managed watering regime and the gradual conditioning of shoots from the day of setting enhanced survival and rooting. Removal of basal callus from shoots prior to an auxin treatment significantly improved survival (30–70%) and rooting (16–56%). A further improvement was made by providing a 5 week prerooting treatment of nutrient medium containing 6% sucrose. This pretreatment was particularly effective for one difficult to root clone. Shoots formed vigorous plantlets which were successfully acclimatised to full sunlight within 4–6 weeks of transfer to root trainers. Plantlet survival ranged from 90 to 100%.

Elongation, rooting and acclimatization of micropropagated shoots from mature material of hybrid larch

“…The conditions favoring rooting of micropropagated shoots from mature material are much more specific than for juvenile shoots (Horgan & Holland, 1989). Elongated shoots rooted following a first treatment with NAA and IBA for root induction (RI), and a second one without growth regulators for root elongation (RE).…”

Evaluation of Clonal Variability in Shoot Coppicing Ability and in vitro Responses of Dalbergia sissoo Roxb

“…Clones 12 and 40 exhibited higher rooting percentages as well as number. Role of Kalia et al·Silvae Genetica (2004) 53-5/6, 212-220 genotypic variations in rooting has been encountered in studies of different research groups (HORGAN and HOLLAND, 1989;BERGMANN and STOMP, 1994;SCALTSOYIANNES et al, 1994). They recorded variation in percentage of shoots rooted and number of roots per shoot.…”

Detection of asymptomatic fungal microorganisms in Pinus radiata tissue culture material

“…Non-germinated hypocotyls (including epicotyls) from the embryos that provided the cotyledons for cryopreservation were retained and these provided the noncryopreserved epicotyl-axillary shoots, thus material was available from two methodology origins using the same genotypes (Hargreaves et al 2004(Hargreaves et al , 2005. Mature material was established using techniques described by Horgan and Holland (1989).…”