ROOTING OF MINICUTTINGS OF Castanea sativa Mill. HYBRID CLONES

Dantas, Ângelo Kidelman; Majada, Juan; Dantas, Fernando Kidelmar; Delatorre, Carolina; Granda, Víctor; Vallejo, Paula; Feito, Isabel

doi:10.1590/0100-67622016000300010

Cited by 11 publications

(5 citation statements)

References 17 publications

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“…At optimal conditions established in each of these studies, longer cuttings performed better than the shorter ones [ 64 , 65 ]. Rooting frequencies affected by both auxin and genotype were reported in minicuttings of Castanea hybrids [ 88 ]. The genetic control on AR has been reported in juvenile chestnut cuttings of different full-sib families [ 10 ], in which both the presence and the number of roots traits were mainly controlled by non-additive variance.…”

Section: Main Factors Influencing the Rooting Responsementioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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The genus Castanea includes several tree species that are relevant because of their geographical extension and their multipurpose character, that includes nut and timber production. However, commercial exploitation of the trees is hindered by several factors, particularly by their limited regeneration ability. Regardless of recent advances, there exists a serious limitation for the propagation of elite genotypes of chestnut due to decline of rooting ability as the tree ages. In the present review, we summarize the research developed in this genus during the last three decades concerning the formation of adventitious roots (ARs). Focusing on cuttings and in vitro microshoots, we gather the information available on several species, particularly C. sativa, C. dentata and the hybrid C.sativa × C. crenata, and analyze the influence of several factors on the achievements of the applied protocols, including genotype, auxin treatment, light regime and rooting media. We also pay attention to the acclimation phase, as well as compile the information available about biochemical and molecular related aspects. Furthermore, we considerate promising biotechnological approaches that might enable the improvement of the current protocols.

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Section: Main Factors Influencing the Rooting Responsementioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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“…In chestnut, callus formation at the shoot base was reduced at high concentrations (1-10 µM) of MDPUs, but the effect was dependent on the auxin concentration. Although callus formation was negatively correlated with rooting percentage in chestnut and other species (Dantas et al 2016), in the experimental system used here the presence of callus did not have a detrimental effect on rooting percentage.…”

Section: Discussionmentioning

confidence: 58%

“…In addition to being affected by auxin, the maturation stage and other factors that regulate rooting, AR competence is a quantitative trait (Riemenschneider 1993) and is thus highly genotype-dependent. Genotypic differences in the rooting ability as well as in the rooting response of shoots to a particular inductive treatment have been reported in chestnut and oak (Dantas et al 2016;Osterc et al 2006;Sánchez et al 1996;Sánchez et al 1997b).…”

Section: Introductionmentioning

confidence: 96%

“…Moreover, under the same IBA treatment cuttings from mature tissues form callus rather than roots. A balance between the formation of ARs and callus is required because an inverse relationship between the presence of callus formation and rooting has been reported in chestnut (Osterc et al 2006;Dantas et al 2016) and other species (Abu-Zabra et al 2012). Callus formation may also interfere in the establishment of the vascular connection between the root and the stem.…”

Section: Introductionmentioning

confidence: 99%

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Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Vielba

Vidal

Ricci

et al. 2020

Israel J. Plant Sci.

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The present study investigated how auxin concentration and the method of application affected the formation of adventitious roots in microshoots of chestnut (Castanea sativa) and oak (Quercus robur). The activity of two urea derivatives (2, 3-MDPU and 3, 4-MDPU) was also evaluated. Microshoots were derived from basal sprouts of two mature chestnut trees (P1 and P2) and one adult oak genotype (Sainza). In chestnut, rooting percentage was positively affected by auxin in a dose-dependent manner, particularly in shoots treated with the hormone for 24 h. The effect of auxin on rooting also differed depending on the application method. In shoots treated for 24 h, the highest concentration of auxin produced the healthiest rooted plantlets, in terms of the root system and shoot quality. By contrast, in shoots treated by the basal quick-dip method, the shoot quality was best at the lowest auxin concentration. The effect of urea derivatives on the root system depended on the species as well as on the auxin concentration and application period. Use of the MDPUs improved the root system architecture of auxin-treated shoots by promoting lateral root development and triggering the synchronous initiation of root primordia at the base of the shoot. Shoot quality was also improved by MDPUs, which promoted resumption of growth and reduced shoot-tip necrosis.

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“…The samples used for this were leaves from hybrid clones of Castanea crenata × C. sativa (third growing season) grown under controlled conditions (25 ℃ ± 2; 40% ± 10 relative humidity, and long photoperiod of 16 h at 52 µmol m −2 s −1 ) (Dantas et al., 2016). After collection, samples were stored at three different temperatures (–80 ℃, –20 ℃, and 4 ℃) for 18–21 days.…”

Section: Methodsmentioning

confidence: 99%

Methodology optimization for the analysis of phenolic compounds in chestnut (Castanea sativa Mill.)

Fuente-Maqueda

Rodrı́guez

Majada

et al. 2020

Food sci. technol. int.

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Phenols are bioactive substances of great interest because of their involvement in plant physiology, their use in many industrial processes, and their impact on human health. This work aims to summarize the varied approaches to the phenolic analysis of chestnut (bark and wood of trunk and branches, leaves, catkins, burs, and fruit) and to collate the optimal conditions into an easy to follow and execute protocol. Phenolic compounds were extracted by solid–liquid extraction and separated by liquid–liquid extraction. Total phenols content was determined by Folin–Ciocalteu assay, condensed tannins by vanillin assay, and hydrolyzable tannins (gallotannins and ellagitannins) by high-performance liquid chromatography quantification of methyl gallate and ellagic acid following acid methanolysis. The lowest temperature for conservation (–80 ℃), lyophilization, and milling (liquid N2) were the most effective pretreatments for samples. For quantification of tannins, the use of water clearly reduced the sensitivity of the analysis of condensed tannins, whilst the more efficient degradation capacity of sulfuric acid improved the methanolysis of hydrolyzable tannins. These findings were validated using a range of chestnut tissues, and thus confirm the utility and effectiveness of this easy to implement, cost-effective, and efficient protocol.

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ROOTING OF MINICUTTINGS OF Castanea sativa Mill. HYBRID CLONES

Cited by 11 publications

References 17 publications

Recent Advances in Adventitious Root Formation in Chestnut

Recent Advances in Adventitious Root Formation in Chestnut

Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Methodology optimization for the analysis of phenolic compounds in chestnut (Castanea sativa Mill.)

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