Azadirachta indica is an evergreen woody plant of Meliaceae family, native to Indian subcontinent and distributed in the tropical and subtropical regions of the world. For efficient multiplication and conservation of the genetic resources of neem, the effect auxin, their different concentration in different rooting media on adventitious root formation (ARF) in semi-hard wood cuttings of Azadirachta indica was studied. Three different rooting media (sand, vermiculite and soil) were used and the experiment was established using three types of auxin (IBA, IAA and NAA) and 6 concentration (100, 250, 500, 750, 1000 and 1500 mg L-1), in a complete randomized block design (CRBD). Significant effects of auxin, their concentration and rooting media on adventitious root formation of neem semi hard wood cuttings were observed. Semi hard wood cuttings were assessed for rooting percentage, number of sprouts, number of roots, root length and number of leaves. Data revealed that there was significant effect (p < 0.05) of auxin-vermiculite on rooting percentage, number of sprouts, number of root, length of root and number of leaves. The data showed that a maximum of 65% rooting with 3.00 numbers of sprout, 32.38 number of roots with 5.77 cm root length and 4.92 numbers of leaves was obtained, when these cuttings were treated with 500 ppm IBA. The determination of proper rooting protocols and the use of semi hard wood cuttings were proved important for multiplication of A. indica. The rooted plantlets were successfully hardened and acclimatized in poly house and agro shade house. These plants showed a good survival rate of 80% under field conditions.