Rootstock rescues watermelon from Fusarium wilt disease by shaping protective root-associated microbiomes and metabolites in continuous cropping soils

Ge, An‐Hui; Liang, Zhenhai; Han, Li‐Li; Xiao, Jianru; Zhang, Yi; Zeng, Qing; Xiang, Ji-Fang; Xiong, Chao; Zhang, Limei

doi:10.1007/s11104-022-05532-z

Cited by 10 publications

(10 citation statements)

References 89 publications

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“…This experiment investigated the metabolome of the sample by LC−MS/MS. The specific procedure was as follows: (1) The original data obtained were converted to mzXML format (xcms input file format) using Proteowizard software (v3.0.8789). (2) The R (v3.1.3) XCMS package was used to perform peak identification, peak filtering, and peak alignment.…”

Section: Discussionmentioning

confidence: 99%

Integrated metagenomics and metabolomics analysis reveals changes in the microbiome and metabolites in the rhizosphere soil of Fritillaria unibracteata

Liu

Ying

et al. 2023

Front. Plant Sci.

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Fritillaria unibracteata (FU) is a renowned herb in China that requires strict growth conditions in its cultivation process. During this process, the soil microorganisms and their metabolites may directly affect the growth and development of FU, for example, the pathogen infection and sipeimine production. However, few systematic studies have reported the changes in the microbiome and metabolites during FU cultivation thus far. In this work, we simultaneously used metagenomics and metabolomics technology to monitor the changes in microbial communities and metabolites in the rhizosphere of FU during its cultivation for one, two, and three years. Moreover, the interaction between microorganisms and metabolites was investigated by co-occurrence network analysis. The results showed that the microbial composition between the three cultivation-year groups was significantly different (2020-2022). The dominant genera changed from Pseudomonas and Botrytis in CC1 to Mycolicibacterium and Pseudogymnoascus in CC3. The relative abundances of beneficial microorganisms decreased, while the relative abundances of harmful microorganisms showed an increasing trend. The metabolomics results showed that significant changes of the of metabolite composition were observed in the rhizosphere soil, and the relative abundances of some beneficial metabolites showed a decreasing trend. In this study, we discussed the changes in the microbiome and metabolites during the three-year cultivation of FU and revealed the relationship between microorganisms and metabolites. This work provides a reference for the efficient and sustainable cultivation of FU.

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Section: Discussionmentioning

confidence: 99%

Integrated metagenomics and metabolomics analysis reveals changes in the microbiome and metabolites in the rhizosphere soil of Fritillaria unibracteata

Liu

Ying

et al. 2023

Front. Plant Sci.

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“…More recently, the plant‐associated microbiome, especially the root microbiome, is regarded as the plant second genome and can extend the plant phenotype through various mechanisms, making it crucial to promote plant health and growth (Liu et al, 2021). It has been reported that the plant root fungi, shaped by host filtering processes, exhibited a lower diversity and a distinctive community composition in comparison to soil (Chen et al, 2020), and functioned synchronously from soil to root for the benefit of the host plant (Ge et al, 2022). Previous studies suggested that root fungi were less affected by fertilization than soil fungi, as the host genetics primarily determined the root‐associated microbiomes of crops (Sun et al, 2020; Xiong et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Enrichment of keystone fungal taxa after flue gas desulphurization gypsum application drives reclamation of the saline‐sodic soil

Zhang

Tóth

et al. 2023

Land Degrad Dev

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Soil salinization is a major threat to sustainable agriculture in the western part of Songnen Plain, Northeast China. Flue gas desulphurization gypsum (FGDG) is commonly used for reclaiming of saline soils due to its high efficiency and cost-effectiveness. However, the responses of soil and root-associated fungi after FGDG amendment and the keystone fungal taxa in determining soil reclamation effect remain elusive. In this study, a long-term field experiment was conducted to explore the effect of different levels of one-time FGDG application on the soil reclamation and the herbage (Leymus chinensis) growth. Our results showed that the FGDG application greatly reduced soil salinity level, increased soil fertility and fungal diversity, and promoted aboveground biomass of L. chinensis, whereas reduced FGDG dose to 60% of gypsum requirement (GR) exhibited non-significant difference on these characters compared with 100% GR treatment. We further discovered that both soil and root fungal communities were greatly changed after FGDG application, with root fungal community more sensitive than soil fungi. Through applying the Random Forest model, the arbuscular mycorrhizal fungi Glomeromycota was identified as the keystone taxa in predicting the soil salinity, soil fertility, and plant growth trait. Intriguingly, some Glomeromycota-affiliated ZOTUs were consistently enriched in the FGDG application treatments, and acted as keystone hubs in the co-occurrence network of fungal communities. Together, our study provides new insights that the enrichment of keystone Glomeromycota after FGDG application determines the reclamation of the saline-sodic soil, which would guide the microbial-based strategy for the development of degraded ecosystems.

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“…Despite this variation, tomato grafting, overall, caused a 37% yield increase for all studies combined; an effect size that is nearly identical to what we observed (+38%) in the final year of the study. Recent studies also emphasize that grafted plants can strengthen beneficial microbial associations in the rhizosphere (Ge et al, 2022; Ruan et al, 2020), including in MF‐grafted tomato (Poudel et al, 2019). This opens the possibility that grafting benefited tomato growth and yield in our experiment via the microbiome.…”

Section: Discussionmentioning

confidence: 99%

Integrating grafting and companion planting to improve crop performance in intensive high‐tunnel tomato production

Ghanem,

Kaplan

2023

J of Sust Agri & Env

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IntroductionTomatoes are among the most popular horticultural crops cultivated in high tunnels. Due to space limitations in these enclosed environments, some tomatoes are produced in succession (i.e., without rotation) across years, which could lead to yield reductions over time. However, the impact of tomato monocultures on productivity in high tunnels and strategies to buffer against yield declines need further testing.Materials and MethodsWe used a 4‐year field experiment whereby heirloom tomatoes (cv. Cherokee Purple and Black Krim) were grown in the same high tunnel soil over time. We tested the impact of both tomato grafting and companion planting on aboveground vegetative biomass and fruit yield. For the grafting treatment, we used the commercially available Maxifort and wild tomato, Solanum pimpinellifolium, as rootstocks. For companion planting, we seeded open alleys with clover and spatially shifted crop beds each year such that tomatoes were transplanted into the previous year's clover planting.ResultsOur data indicate that the companion (clover) treatment had little to no impact on tomato production, while grafting to Maxifort rootstock had a significant positive effect on biomass and yield. However, beneficial effects of grafting were only observed over time, in years three (+71% inc. in biomass) and four (+77% inc. in biomass, +38% inc. in yield) of the experiment; not during the initial 2 years. Leaf (SPAD) and fruit (Brix) metrics were unaffected by any of the treatments.ConclusionOverall, our results suggest that grafting tomatoes with commercially available rootstock is an effective tool for maintaining production in high tunnel monocultures over time.

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Rootstock rescues watermelon from Fusarium wilt disease by shaping protective root-associated microbiomes and metabolites in continuous cropping soils

Cited by 10 publications

References 89 publications

Integrated metagenomics and metabolomics analysis reveals changes in the microbiome and metabolites in the rhizosphere soil of Fritillaria unibracteata

Integrated metagenomics and metabolomics analysis reveals changes in the microbiome and metabolites in the rhizosphere soil of Fritillaria unibracteata

Enrichment of keystone fungal taxa after flue gas desulphurization gypsum application drives reclamation of the saline‐sodic soil

Integrating grafting and companion planting to improve crop performance in intensive high‐tunnel tomato production

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