Grapevines, an economic mainstay of the Douro Demarcated Region, are under increasing stressful conditions and they can suffer further losses due to climate change. Observations on weather patterns and behavior of two autochthonous grapevines and two exotic ones were made over several years. There are indications of an increase of 2°C from 2003 to 2019 responsible for the advancement of 10 to 15 days of phenological events on all grape varieties, a clear biological sign of climate change. Against the forecasted trends, rainfall showed a trend for increasing total amount but a lower proportion during the growing season that resulted in stronger seasonality. The yields of native varieties were about 2600 kg ha−1 higher than exotic varieties, a difference supported by a larger leaf area, on average 1.7 m2 higher, and better stomatal conductance in average 2.6 mm s−1 and 2.1 mm s−1 for native and exotic varieties, respectively. These differences suggest that natives are better suited to withstand aggravated environmental conditions than the exotic. The composition of the must show significantly higher total soluble content in autochthonous grapevines but they have a lower concentration of organic acids, tannins, and polyphenols, meaning poorer organoleptic profiles.