Rosa davurica Pall. Improves Propionibacterium acnes-Induced Inflammatory Responses in Mouse Ear Edema Model and Suppresses Pro-Inflammatory Chemokine Production via MAPK and NF-κB Pathways in HaCaT Cells

Hwang, Du Hyeon; Lee, Dong Yeol; Koh, Phil-Ok; Yang, Hye Ryeon; Kang, Changkeun; Kim, Euikyung

doi:10.3390/ijms21051717

Cited by 19 publications

(12 citation statements)

References 43 publications

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“…Thus, we concluded that SMYEE might be a safe and effective acne treatment. We evaluated the effects of SMYEE on the C. acnes-induced inflammatory response in vivo using a well-known mouse acne model; the lesions are similar to those of humans [26]. C. acnes injection into mouse dorsal skin induces nodule development, swelling, redness, erythema, epidermal hyperplasia and thickening, as well as the accumulation of inflammatory cells (especially neutrophils).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of a Sargassum miyabei Yendo on Cutibacterium acnes-Induced Skin Inflammation

et al. 2020

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Acne vulgaris is a chronic inflammatory condition of skin sebaceous follicles. To explore its effects on acne vulgaris, we investigated the antibacterial and anti-inflammatory activities of Sargassum miyabei Yendo (a brown alga) ethanolic extract (SMYEE) on Cutibacterium acnes (C. acnes)-stimulated inflammatory responses, both in vivo and in vitro. To induce inflammation in vivo, C. acnes was intradermally injected into the dorsal skin of mice, to which SMYEE was applied. The antimicrobial activity of SMYEE was evaluated by the determination of minimum inhibitory concentrations (MICs). To explore in vitro anti-inflammatory effects, HaCaT cells were stimulated with C. acnes after treatment with SMYEE. The levels of IL-8 and the underlying molecular effects in C. acnes-stimulated HaCaT cells were assessed by enzyme-linked immunosorbent assay, Western blotting, and an electrophoretic mobility shift assay. Mouse skin lesions improved after treatment with SMYEE (50 μg/mouse). Neutrophil infiltration was significantly reduced in SMYEE-treated compared to SMYEE-untreated skin lesions. SMYEE reversed the C. acnes-induced increase in IL-8 levels in HaCaT cells and suppressed dHL-60 cell migration. SMYEE also inhibited C. acnes-induced phosphorylation of the extracellular signal-regulated kinase and inhibited activator protein-1 signaling. SMYEE may be a useful treatment for C. acnes-induced acne vulgaris.

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Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of a Sargassum miyabei Yendo on Cutibacterium acnes-Induced Skin Inflammation

et al. 2020

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“…Since many previous studies have reported that activation of NF-κB and MAPK signaling pathways is involved in TNF-α-induced expression of proinflammatory mediators in keratinocytes (Razali et al 2018 ; Sikandan et al 2018 ; Hwang et al 2020 ), we further examined the effect of FB801 on TNF-α-induced IκB-α degradation and phosphorylation of MAPK by Western blot analysis. Stimulation of HaCaT cells with TNF-α (10 ng/mL, 15 min) rapidly induced IκB-α degradation and MAPK phosphorylation, as shown in Figure 6 .…”

Section: Resultsmentioning

confidence: 99%

Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice

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et al. 2021

Animal Cells and Systems

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Zea mays L. (Poaceae), also known as purple corn, is an annual herbaceous plant that is grown as food for human consumption in a variety of forms, including cooking oils and sweeteners in processed food and beverage products. The purpose of this study was to determine whether a novel purple corn extract, FB801, might have an anti-atopic dermatitis (AD) effect on AD-like skin lesions induced by 2,4-dinitrochlorobenzene (DNCB) in BALB/c mice. Topical sensitization (1%) and challenge (0.3%) by DNCB were performed on the dorsal skin and right ear of BALB/c mice to induce AD. Following FB801 and dexamethasone administered orally, the severity of skin lesions was examined macroscopically and histologically. Serum levels of immunoglobulin E (IgE) and various cytokines were determined by enzyme-linked immunosorbent assay. Oral administration of FB801 significantly reduced typical symptoms of AD (erythema/bleeding, swelling, molting/erosion and scaling/drying), scratching frequencies, and the recruitment of inflammatory and mast cells. In addition, FB801 suppressed serum levels of IgE and T helper (Th)2 type cytokines such as interleukin (IL)-4 and IL-10 in DNCB-treated BALB/c mice. Furthermore, FB801 reduced the degradation of inhibitor of nuclear factor-κB proteins (NF-κB) in tumor necrosis factor (TNF)-α-stimulated human keratinocyte (HaCaT) cells. These results suggest that FB801 inhibited the development of the AD-like skin symptoms by regulating Th1 and Th2 responses in the skin lesions in mice and suppressing TNF-α induced NF-κB activation in HaCaT cells, suggesting that FB801 has potential application as an effective alternative therapy for the prevention and management of AD.

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“…The inflammatory response induced by C. acnes is mediated by the pattern recognition receptor Toll-like receptor-2 (TLR2) expressed in keratinocytes and APCs [ 47 ]. In vitro studies demonstrated that upon the C. acnes challenge, the mitogen-activated protein kinase (MAPK) and nuclear factor-kB (NF-kB) pathways are activated leading to the production of TNF-α, IL-8, IL-6, and IL-1β by HaCaT cells [ 48 ] and THP-1 macrophages [ 49 ]. In line with those findings, it was shown that the C. acnes -induced inflammatory response promotes the accumulation of neutrophils [ 50 ] and lymphocytes [ 51 ] in the sebaceous follicles, where they can promote the breakdown of the follicular wall, stimulating further inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…In order to demonstrate the potential beneficial effects of orally administered W. viridescens UCO-SMC3, we next used a mice model of acne for in vivo studies. Most of the studies using mice to evaluate C. acnes infection usually inject the pathogen into the animal’s ears [ 48 , 62 , 63 ]. C. acnes inoculated subcutaneously into the back of the ears induce ear swelling, redness, and erythema [ 48 ], which are associated with the local and systemic production of TNF-α, IL-6, and IL-8 [ 63 ] as well as the infiltration of CD45 + Ly6G + neutrophils and CD45 + F4/80 + macrophages into the infected tissue [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

“…Most of the studies using mice to evaluate C. acnes infection usually inject the pathogen into the animal’s ears [ 48 , 62 , 63 ]. C. acnes inoculated subcutaneously into the back of the ears induce ear swelling, redness, and erythema [ 48 ], which are associated with the local and systemic production of TNF-α, IL-6, and IL-8 [ 63 ] as well as the infiltration of CD45 + Ly6G + neutrophils and CD45 + F4/80 + macrophages into the infected tissue [ 62 ]. Although this model is useful for studies of therapeutic options for acne, it has limitations associated with the small size of the animals’ ears.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Weissella viridescens UCO-SMC3 as a Potential Probiotic for the Skin: Its Beneficial Role in the Pathogenesis of Acne Vulgaris

et al. 2021

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Previously, we isolated lactic acid bacteria from the slime of the garden snail Helix aspersa Müller and selected Weissella viridescens UCO-SMC3 because of its ability to inhibit in vitro the growth of the skin-associated pathogen Cutibacterium acnes. The present study aimed to characterize the antimicrobial and immunomodulatory properties of W. viridescens UCO-SMC3 and to demonstrate its beneficial effect in the treatment of acne vulgaris. Our in vitro studies showed that the UCO-SMC3 strain resists adverse gastrointestinal conditions, inhibits the growth of clinical isolates of C. acnes, and reduces the adhesion of the pathogen to keratinocytes. Furthermore, in vivo studies in a mice model of C. acnes infection demonstrated that W. viridescens UCO-SMC3 beneficially modulates the immune response against the skin pathogen. Both the oral and topical administration of the UCO-SCM3 strain was capable of reducing the replication of C. acnes in skin lesions and beneficially modulating the inflammatory response. Of note, orally administered W. viridescens UCO-SMC3 induced more remarkable changes in the immune response to C. acnes than the topical treatment. However, the topical administration of W. viridescens UCO-SMC3 was more efficient than the oral treatment to reduce pathogen bacterial loads in the skin, and effects probably related to its ability to inhibit and antagonize the adhesion of C. acnes. Furthermore, a pilot study in acne volunteers demonstrated the capacity of a facial cream containing the UCO-SMC3 strain to reduce acne lesions. The results presented here encourage further mechanistic and clinical investigations to characterize W. viridescens UCO-SMC3 as a probiotic for acne vulgaris treatment.

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Rosa davurica Pall. Improves Propionibacterium acnes-Induced Inflammatory Responses in Mouse Ear Edema Model and Suppresses Pro-Inflammatory Chemokine Production via MAPK and NF-κB Pathways in HaCaT Cells

Cited by 19 publications

References 43 publications

Inhibitory Effects of a Sargassum miyabei Yendo on Cutibacterium acnes-Induced Skin Inflammation

Inhibitory Effects of a Sargassum miyabei Yendo on Cutibacterium acnes-Induced Skin Inflammation

Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice

Characterization of Weissella viridescens UCO-SMC3 as a Potential Probiotic for the Skin: Its Beneficial Role in the Pathogenesis of Acne Vulgaris

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