Rosa Mosqueta Oil Prevents Oxidative Stress and Inflammation through the Upregulation of PPAR-α and NRF2 in C57BL/6J Mice Fed a High-Fat Diet

Gonzalez‐Mañán, Daniel; D'Espessailles, Amanda; Dossi, Camila G.; Martín, Marcela San; Mancilla, Rodrigo A.; Tapia, Gladys

doi:10.3945/jn.116.243261

Cited by 31 publications

(17 citation statements)

References 53 publications

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“…Furthermore, there was an increased lipid peroxidation in the liver in response to steatosis and excess substrate (lipids) for peroxidation, and tissue oxidative damage was evidenced by histopathological alterations in the liver and increases in plasmatic liver health-related biomarkers, AST and ALT. The results described herein corroborate previous studies such as those carried out by Sarna et al [49], González-Mañán et al [50], and Venezuela et al [22], who indicated impaired glucose metabolism, increased hepatic liver injury enzyme markers, lipid peroxidation, and reduction in antioxidant enzyme activities in high-fat fed animals.…”

Section: Discussionsupporting

confidence: 92%

Short-Term Betanin Intake Reduces Oxidative Stress in Wistar Rats

et al. 2019

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Oxidative stress is a common condition described in risk factors for cardiovascular disease. Betanin, a bioactive pigment from red beetroot demonstrates anti-inflammatory and antioxidant properties. The main aim of this study was to evaluate the short-term intake of betanin against oxidative stress in a rodent model, a common condition described in several risk factors for cardiovascular disease. Oxidative stress was induced in Wistar rats by a hyperlipidemic diet for 60 days, followed by betanin administration (20 mg·kg−1) through oral gavage for 20 days. Plasma biochemical parameters and antioxidant enzyme activities were evaluated. Lipid peroxidation and histopathological changes were determined in the liver. The hyperlipidemic diet caused hyperglycemia, hyperinsulinemia, insulin resistance, and increases in alanine transaminase and aspartate transaminase levels. Oxidative stress status was confirmed by reduction of antioxidant enzyme activities, increased lipid peroxidation, and liver damage. Purified betanin regulated glucose levels, insulin, and insulin resistance. Hepatic damage was reversed as evidenced by the reduction in alanine transaminase and aspartate transaminase levels and confirmed by histological analyses. Betanin reduced hepatic malondialdehyde and increased superoxide dismutase, catalase, and glutathione peroxidase activities. Short-term betanin intake modulated biochemical parameters, reversed hepatic tissue damage, and attenuated oxidative stress in Wistar rats.

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Section: Discussionsupporting

confidence: 92%

Short-Term Betanin Intake Reduces Oxidative Stress in Wistar Rats

et al. 2019

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“…The binding of oestrogen to membrane ER directly phosphorylates PPAR- α and increases the intracellular concentration of DHA, which indirectly activates PPAR- α via elevated Ca phospholipase A 2 ( 7 ) . The present study and previous studies ( 21 , 39 ) consistently showed that low-LA diets increase PPAR- α expression. Therefore, the combination of oestrogen and a low-LA diet have an added effect on PPAR- α expression.…”

Section: Discussionsupporting

confidence: 91%

Low-linoleic acid diet and oestrogen enhance the conversion ofα-linolenic acid into DHA through modification of conversion enzymes and transcription factors

2018

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Conversion of α-linolenic acid (ALA) into the longer chain n-3 PUFA has been suggested to be affected by the dietary intake of linoleic acid (LA), but the mechanism is not well known. Therefore, the purpose of this study was to evaluate the effect of a low-LA diet with and without oestrogen on the fatty acid conversion enzymes and transcription factors. Rats were fed a modified American Institute of Nutrition-93G diet with 0% n-3 PUFA or ALA, containing low or high amounts of LA for 12 weeks. At 8 weeks, the rats were injected with maize oil with or without 17β-oestradiol-3-benzoate (E) at constant intervals for the remaining 3 weeks. Both the low-LA diet and E significantly increased the hepatic expressions of PPAR-α, fatty acid desaturase (FADS) 2, elongase of very long chain fatty acids 2 (ELOVL2) and ELOVL5 but decreased sterol regulatory element binding protein 1. The low-LA diet, but not E, increased the hepatic expression of FADS1, and E increased the hepatic expression of oestrogen receptor-α and β. The low-LA diet and E had synergic effects on serum and liver levels of DHA and on the hepatic expression of PPAR-α. In conclusion, the low-LA diet and oestrogen increased the conversion of ALA into DHA by upregulating the elongases and desaturases of fatty acids through regulating the expression of transcription factors. The low-LA diet and E had a synergic effect on serum and liver levels of DHA through increasing the expression of PPAR-α.

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“…Keap1 has been identified as a cytosolic binding protein for Nrf2, which is associated with the Kelch domain of Keap1. Keap1 is sequestered in association with the actin cytoskeleton under normal physiological conditions, which in turn allows for proteasome degradation of Nrf2 [ 35 ]. In the present study, the Nrf2 protein expression level was increased and the Keap1 protein expression level was decreased, which indicated that the Nrf2 pathway was activated by dietary resveratrol supplementation.…”

Section: Discussionmentioning

confidence: 99%

Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1

Meng

Guo

et al. 2018

J Animal Sci Biotechnol

123

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BackgroundResveratrol, a plant phenol, affords protection against inflammation and oxidative stress. The objective of this study was to investigate the effects of dietary resveratrol supplementation during pregnancy and lactation on the antioxidant status of sows and piglets and on antioxidant gene expression and pathway in placenta.MethodsForty sows were allotted to 2 dietary treatments 20 d after breeding. Sows were fed a control diet and a control diet with 300 mg/kg resveratrol. Oxidative stress biomarkers and antioxidant enzymes were measured in the placenta, milk, and plasma of sows and piglets. Antioxidant gene expression and protein expression of Kelch-like ECH-associated protein 1-Nuclear factor E2-related factor 2 (Keap1-Nrf2), nuclear factor kappa B-p65 (NFκB-p65) and sirtuin1 (Sirt1) were quantified in the placenta.ResultsDietary resveratrol increased the litter and piglets weaning weights. Antioxidant status in the milk, placenta and plasma of sows and piglets was partially improved by dietary resveratrol. In placenta, Nrf2 protein expression was increased and Keap1 protein expression was decreased by dietary resveratrol. The mRNA expression of antioxidant genes including catalase (CAT), glutathione peroxidase 1 (GPX1), GPX4, superoxide dismutase 1 (SOD1) and heme oxygenase 1 (HO1), and phase 2 detoxification genes, including glutamate-cysteine ligase modifier (GCLM), microsomal glutathione S-transferase 1(MGST1) and UDP glucuronosyltransferase family 1 member A1 (UGT1A1), was increased by dietary resveratrol. Dietary resveratrol also increased Sirt1 and phosphorylated NFκB-p65 protein expression in the placenta. We failed to observe any influences of dietary resveratrol on pro-inflammatory cytokine levels, including those of interleukin 1β (IL-1β), IL-6, IL-8 and tumor necrosis factor α (TNF-α). However, we observed that the mRNA expression of IL-8 in placenta was reduced by maternal resveratrol. In addition, dietary resveratrol showed interactive effects with day of lactation on activities of SOD and CAT and levels of malonaldehyde (MDA) and hydrogen peroxide (H2O2) in milk.ConclusionsDietary resveratrol supplementation during pregnancy and lactation improves the antioxidant status of sows and piglets, which is beneficial to the reproductive performance of sows. Dietary resveratrol regulates placental antioxidant gene expression by the Keap1-Nrf2 pathway and Sirt1 in placenta.

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Rosa Mosqueta Oil Prevents Oxidative Stress and Inflammation through the Upregulation of PPAR-α and NRF2 in C57BL/6J Mice Fed a High-Fat Diet

Cited by 31 publications

References 53 publications

Short-Term Betanin Intake Reduces Oxidative Stress in Wistar Rats

Short-Term Betanin Intake Reduces Oxidative Stress in Wistar Rats

Low-linoleic acid diet and oestrogen enhance the conversion ofα-linolenic acid into DHA through modification of conversion enzymes and transcription factors

Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1

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