Rose Bengal Photodynamic Antimicrobial Therapy as potential adjuvant treatment for Serratia marcescens corneal ulcer

Levine, Harry; Sepulveda-Beltran, Paula A.; Amescua, Guillermo

doi:10.1016/j.ajo.2021.07.007

Cited by 2 publications

(5 citation statements)

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“…In the current study, we proved that viability and proliferation of HCFs and KC-HCFs is strongly affected by the combination of RB and green light, in a fluence dependent manner. Our results showed that the viability of primary HCFs and KC-HCFs started to decrease from 0.17 J/cm 2 , which is much lower than the applied energy setting of RB-PDT in animal models (150 J/cm 2 ) [8,37], and also much lower than the 5.4 J/cm 2 fluence during clinical application of rose bengal antimicrobial therapy [38]. Through increasing the fluence to 0.7 J/cm 2 , nearly all cells lost their viability in culture.…”

Section: Discussionmentioning

confidence: 66%

Assessment of Rose Bengal Photodynamic Therapy on Viability and Proliferation of Human Keratolimbal Epithelial and Stromal Cells In Vitro

Chai

Stachon

Nastaranpour

et al. 2023

Klin Monbl Augenheilkd

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ZUSAMMENFASSUNG Ziel: Die photodynamische Therapie kann eine alternative Behandlungsmethode bei antibiotikaresistenten Keratitiden darstellen. In dieser Studie wurde die Wirkung der photodynamischen Therapie mit Bengalrosa (RB-PDT) auf die Viabilität und Proliferation von humanen limbalen Epithelstammzellen (T-LSCs), humanen Hornhautepithelzellen (HCE-T), limbalen Fibroblasten (LFCs), normalen und Keratokonus-Fibroblasten (HCFs und KC-HCFs) „in vitro“ untersucht. Material und Methoden: Für die Experimente an den limbalen Epithelstammzellen wurden 2 verschiedene Zelllinien (T-LSCs und HCE-T) verwendet. Die primären LFCs und HCFs wurden aus den Korneoskleralringen von Hornhautspendern, die KC-HCFs aus perforierenden Keratoplastiken von Keratokonus-Patienten isoliert und kultiviert (jeweils n=5). Die RB-PDT wurde mit einer 0,001% igen RB-Konzentration bei einer Wellenlänge von 565 nm und einer Energiedosis von 0,14 bis 0,7 J/cm2 durchgeführt. Zur Bestimmung der Viabilität wurde 24 Stunden nach der Bestrahlung der XTT-, zur Bestimmung der Proliferation der BrdU-Assay verwendet. Ergebnisse: Die ausschließliche Verwendung von RB oder Bestrahlung hatten keinen messbaren Einfluss auf die Viabilität oder Proliferation der unterschiedlichen Zelltypen (p≥0,1). Unter Verwendung der RB-PDT mit einer Dosis von 0,17 J/cm2 sank die Viabilität jedoch in den HCFs (p < 0,001) und KC-HCFs (p < 0,0001), und bei einer Dosis von 0,35 J/cm2 in den T-LSCs (p < 0,001), HCE -T (p < 0,05) und LFCs (p < 0,0001). Die Zellproliferation verringerte sich signifikant ab einer Dosis von 0,14 J/cm2 in T-LSCs (p < 0,0001), HCE-T (p < 0,05) und KC- HCFs (p < 0,001) und ab einer Dosis von 0,17 J/cm2 Fluenz in HCFs (p < 0,05). Für die Proliferationsbestimmung der LFCs konnten mit dem BrdU-Assay keine Werte ermittelt werden. Schlussfolgerung: Die RB-PDT zeigt eine dosisabhängige Phototoxizität auf Hornhautepithel- und Stromazellen. Die in dieser Studie ermittelten Daten und experimentellen Parameter bieten eine verlässliche Referenz für zukünftige Untersuchungen für die photodynamische Therapie mit Bengalrosa. Abstract Purpose: To investigate the effect of Rose Bengal photodynamic therapy (RB-PDT) on viability and proliferation of human limbal epithelial stem cells (T-LSCs), human corneal epithelial cells (HCE-T), human limbal fibroblasts (LFCs), human normal and keratoconus fibroblasts (HCFs and KC-HCFs), in vitro. Methods: T-LSCs and HCE-T cell lines were used in this research. LFCs were isolated from healthy donor corneal limbus (n=5), HCFs from healthy human donor corneas (n=5), and KC-HCFs from penetrating keratoplasties of keratoconus patients (n=5). After cell culture, RB-PDT was performed using 0.001% RB concentration and 565 nm wavelength illumination, with 0.14 to 0.7 J/cm2 fluence. The XTT and the BrdU assays were used to assess cell viability and proliferation, 24 hours after RB-PDT. Results: Rose Bengal or illumination alone did not change cell viability or proliferation in any of the cell types (p≥0.1). However, following RB-PDT, viability decreased significantly from 0.17 J/cm2 fluence in HCFs (p<0.001) and KC-HCFs (p<0.0001), from 0.35 J/cm2 fluence in T-LSCs (p<0.001), HCE-T (p<0.05) and LFCs ((p<0.0001), respectively. Cell proliferation decreased significantly from 0.14 J/cm2 fluence in T-LSCs (p<0.0001), HCE-T (p<0.05), and KC-HCFs (p<0.001) and from 0.17 J/cm2 fluence in HCFs (p<0.05). Regarding LFCs proliferation, no values could be determined by the BrdU assay. Conclusions: Though RB-PDT seems to be a safe and effective treatment method in vivo, its dose-dependent phototoxicity on corneal epithelial and stromal cells has to be respected. The data and the experimental parameters applied in this study may provide a reliable reference for future investigations.

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Section: Discussionmentioning

confidence: 66%

Assessment of Rose Bengal Photodynamic Therapy on Viability and Proliferation of Human Keratolimbal Epithelial and Stromal Cells In Vitro

Chai

Stachon

Nastaranpour

et al. 2023

Klin Monbl Augenheilkd

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“…Rose Bengal [76], toluidine blue [77], methylene blue [78], meso-tetra(3-N-methylpyridyl)porphyrin and meso-tetra(4-N-methylpyridyl)porphyrin [52], trans-AB-porphyrin, trans-AB-porphyrin-gelatin nanoparticle conjugate [62].…”

Section: Serratia Marcescens Gnmentioning

confidence: 99%

“…Rose Bengal (6) and eosin Y (7) are xanthene molecules that have been utilized as PSs for aPDT against fish pathogenic bacteria [48, [63][64][65]71,76]. These molecules have, as a result, been utilized as PSs for aPDT [23,24,27,41,43,44,65,75,77,78].…”

Section: Use Of Rose Bengal and Eosin Y As Pss Against Fish Bacterial...mentioning

confidence: 99%

“…Rose Bengal (6) and eosin Y (7) are xanthene molecules that have been utilized as PSs for aPDT against fish pathogenic bacteria [48, [63][64][65]71,76]. uptake of toluidine blue when linked to titanium dioxide (TiO2) and zinc oxide (ZnO) nanoparticles [27].…”

Section: Use Of Rose Bengal and Eosin Y As Pss Against Fish Bacterial...mentioning

confidence: 99%

“…Rose Bengal (6) and eosin Y (7) are xanthene molecules that have been utilized as PSs for aPDT against fish pathogenic bacteria [48,[63][64][65]71,76].…”

Section: Use Of Rose Bengal and Eosin Y As Pss Against Fish Bacterial...mentioning

confidence: 99%

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Applications of Antimicrobial Photodynamic Therapy in Aquaculture: Effect on Fish Pathogenic Bacteria

Dube,

Okuthe

2024

Fishes

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Increased infectious diseases and the reduced effectiveness of antibiotics due to antimicrobial resistance pose global challenges affecting the aquaculture industry. As bacteria increasingly develop antibiotic resistance, research scientists are shifting their focus to technologies such as antimicrobial photodynamic therapy (aPDT), which show potential for treating and controlling fish infections without promoting the development of resistant bacteria. Various photosensitizers (PSs), both natural and synthetic, are under investigation for their application in aPDT within the aquaculture industry. This shift is crucial for the sustainability of the aquaculture industry, which plays a significant role in achieving several of the United Nations (UN) Sustainable Development Goals (SDGs). This review highlights the application of aPDT against fish pathogens in the industry and the types of PSs utilized. It also explores the potential application of this technique for treating and controlling fish infections, along with the advantages and limitations of its use in aquaculture production systems. Finally, a conclusion and future perspectives are provided.

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Rose Bengal Photodynamic Antimicrobial Therapy as potential adjuvant treatment for Serratia marcescens corneal ulcer

Cited by 2 publications

References 0 publications

Assessment of Rose Bengal Photodynamic Therapy on Viability and Proliferation of Human Keratolimbal Epithelial and Stromal Cells In Vitro

Assessment of Rose Bengal Photodynamic Therapy on Viability and Proliferation of Human Keratolimbal Epithelial and Stromal Cells In Vitro

Applications of Antimicrobial Photodynamic Therapy in Aquaculture: Effect on Fish Pathogenic Bacteria

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