Rose-scented geranium (Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality

Saxena, Gauri; Banerjee, Sayan; Laiq-ur-Rahman,; Verma, Praveen Chandra; Mallavarapu, Gopal R.; Kumar, Sushil

doi:10.1007/s11240-007-9261-0

Cited by 41 publications

(13 citation statements)

References 28 publications

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“…(Saxena et al 2007), Gossypium Barbadense (Frankfater et al 2009), Plumbago indica (Gangopadhyay et al 2010), Psoralea drupacea Bge (Lystvan et al 2010), Solanum nigrum L. var. pauciflorum and Drosera capensis var.…”

Section: Discussionmentioning

confidence: 99%

“…So far, hairy roots which grows rapidly with stable and comparatively high content in secondary metabolites, induced by genetic transformation of A. rhizogenes, were successfully used for the production of essential oils from medicinal or aromatic plants, such as Rose-scented geranium (Pelargonium sp.) (Saxena et al 2007), Plumbago indica (Gangopadhyay et al 2010) and Psoralea drupacea (Fabaceae) (Lystvan et al 2010) and for improvement and creation of germplasm for Nierembergia scoparia (Godo et al1997), Kalanchoe blossfeldiana (Christensen et al 2008) and Glycine max (Zia et al 2010). The transgenic plants also produced from hairy roots are usually non-chimeric because the hairy roots originate from single cells and each hairy root consists of uniformly transformed cells.…”

Section: Introductionmentioning

confidence: 99%

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Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Shi

Yong-Yue

Tie-Shan

et al. 2011

Plant Cell Tiss Organ Cult

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An efficient transformation system for the medicinal and aromatic plant, Pogostemon cablin Benth was developed by using agropine-type Agrobacterium rhizogenes ATCC15834. Hairy roots formed directly from the cut edges of leaf explants or via callus stage 8 days after inoculation with the bacterium. The highest frequency of leaf explant transformation by Agrobacterium rhizogenes ATCC15834 was about 80% after infection for 25 days. Hairy roots grew rapidly on plant growth regulators (PGRs)-free Murashige and Skoog (MS) or 6,7-V medium and had characteristics of transformed roots such as fast growth and high lateral branching. The PCR amplification showed that rol genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. The hairy root line, PL6, grew very slowly in the first 8 days, then grew very quickly between day 8 and day 24. The optimum medium for callus induction of hairy roots consisted of 2.0 mg l -1 benzyladenine (BA) and 0.1 mg/l a-naphthaleneacetic acid (NAA); while optimum medium for adventitious shoot regeneration from these cultures consisted of 0.1 mg l -1 BA and 0.1 mg l -1 NAA. Adventitious shoots could be rooted on 1/2MS. Southern blot analysis confirmed that rol genes of TL-DNA of Ri plasmid was integrated with at least three copies into the genome of hairy rootsregenerated P. cablin plants. The results presented provide a solid foundation for production of patchouli essential oil from hairy roots or its regenerated plants and also provide possibilities for utilization of artifical polyploidization or chemical mutation of hairy roots for improving germplasm and breeding of a new cultivar of P. cablin.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Shi

Yong-Yue

Tie-Shan

et al. 2011

Plant Cell Tiss Organ Cult

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“…When kept under continuous light, the hairy roots were found to have an increased metabolite content and to develop greenish nodular buds. Shoots originating from hairy roots have been reported to exhibit an altered morphology, such as curliness of leaves and a shortening of internodes (Casanova et al 2005), which may be desirable for some plant species (Saxena et al 2007;Christensen et al 2008) and undesirable for others (Bhalla and Singh 2008).…”

Section: Introductionmentioning

confidence: 99%

Regeneration of transformed plants from hairy roots of Plumbago indica

Gangopadhyay

Chakraborty

Bhattacharyya

et al. 2010

Plant Cell Tiss Organ Cult

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Spontaneous shoot regeneration was observed from Agrobacterium rhizogenes-induced hairy roots of Plumbago indica when these were incubated in liquid MS medium for a period of 3 weeks under continuous light. Insertion of the rolB gene in putative transformed plants was confirmed by PCR and sequencing. Transformed plants grown for a period of 1 week on solid MS medium containing 0.5 mg l -1 6-benzyladenine and then transplanted to growth regulator-free medium showed better overall growth than control plants. Transformed plants had a higher root bio-biomass and an increased plumbagin content relative to non-transformed plants.

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“…5 So far, hairy roots which grow rapidly with stable and comparatively high content in secondary metabolites, induced by genetic transformation of A. rhizogenes, were successfully used for the production of essential oils from medicinal or aromatic plants, such as Rose-scented geranium (Pelargonium sp. ), 6 Plumbago indica 7 and Psoralea drupacea (Fabaceae) 8 and for improvement and creation of germ-plasm for Nierembergia scoparia, 9 Kalanchoe blossfeldiana 10 and Glycine max. 5 The transgenic plants also produced from hairy roots are usually non-chimeric because the hairy roots originate from single cells and each hairy root consists of uniformly transformed cells.…”

Section: Introductionmentioning

confidence: 99%

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Yan¹,

He²,

Huang³

et al. 2015

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An efficient transformation system for the medicinal and aromatic plant, Pogostemon cablin Benth was developed by using Agrobacterium rhizogenes ATCC15834 and C58C1. Hairy roots formed directly from the cut edges of leaf explants after infection for 2 days. The highest frequency of leaf explant transformation by A. rhizogenes ATCC15834 and C58C1 were 83.3% and 80.5% after pre culture about 2 days and infection by the bacterium containing 15 mg l -1 acetosyringone about 25 min. The PCR amplification showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. The optimum medium for callus induction of hairy roots consisted of 2.0 mg l -1 BA and 0.1 mg l -1 NAA while optimum medium for adventitious shoot regeneration from these cultures consisted of 0.1 mg l -1 BA and 0.1 mg l -1 NAA. Adventitious shoots could be rooted on 1/2MS. PCR analysis confirmed that rol B gene of TL-DNA of Ri plasmid was integrated into the genome of hairy roots-regenerated P. cablin plants. The results presented provide a possibility for breeding of a new cultivar of P. cablin.

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Rose-scented geranium (Pelargonium sp.) generated by Agrobacterium rhizogenes mediated Ri-insertion for improved essential oil quality

Cited by 41 publications

References 28 publications

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Regeneration of transformed plants from hairy roots of Plumbago indica

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

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