Rosehip Oil Promotes Excisional Wound Healing by Accelerating the Phenotypic Transition of Macrophages

Abstract

Poor wound healing is a major and global threat to public health. Efforts have been made to better understand the underlying mechanisms and develop effective remedies, though the advancements that have been made are still limited. As there are no effective and generally applicable therapies available for skin injuries and fibrosis, it is urgent to develop new drugs and therapies that facilitate wound healing and effectively improve scars. In this study, GC-MS analysis was performed to identify the chemical composition of rosehip oil. The excisional wound healing model and the carrageenan-induced paw edema method were respectively applied to evaluate the wound healing activity and anti-inflammatory activity of rosehip oil. Hematoxylin and eosin staining was used to assess the pathological changes of sections, and Sirius-red staining was performed to analyze the ratio of collagen I/III in wound tissues. Immunohistological staining for CD68, CCR7 (CD197), CD163, TGF-1, and -SMA was applied to determine the macrophage phenotypes transition (M1-to-M2) and demonstrate the scar-improving efficacy of rosehip oil on wound healing. Results showed that rosehip oil significantly promoted wound healing and effectively improved scars. This efficacy might be exerted by accelerating the macrophage phenotypes transition and inhibiting the process of epithelial-mesenchymal transition.

Chitosan/Alginate Nanoparticles with Sustained Release of Esculentoside A for Burn Wound Healing

“…With a sustained release of EsA, 5 μg of EsA-CS/ALG-NPs@CCS also inhibited M1 macrophage activation (Figure C, upper lane) and activated the M2 macrophages slightly (Figure C, down lane), compared with the blank CCS. It can be seen that the anti-inflammatory capacity of EsA may be closely related to its role in regulating macrophage polarization, which tends to be effective in promoting wound healing …”

“…It can be seen that the anti-inflammatory capacity of EsA may be closely related to its role in regulating macrophage polarization, which tends to be effective in promoting wound healing. 48 3.3. In Vivo Evaluation of EsA-CS/ALG-NPs@CCS.…”

“…These results indicated that 5 μg EsA-CS/ ALG-NPs@CCS could effectively inhibit the secretion of inflammatory cytokines in vivo. 48 It could be supposed that 5 μg EsA-CS/ALG-NPs@CCS prevents burn wounds from being progressive with the anti-inflammatory capacity, thereby creating a favorable environment for burn wound healing. 13,21 The release of anti-inflammatory factors is conducive to wound injury repair.…”

The Enigma of Bioactivity and Toxicity of Botanical Oils for Skin Care

“…Two months supplementation with black currant seed oil also enhanced the skin immune response and reduced prostaglandin E(2) production (Wu et al, 1999). Sacha inchi and rosehip oils high in a-linolenic acid showed no toxicity toward keratinocytes and moderate inhibition of Staphylococcus aureus adherence in cell culture (Gonzalez-Aspajo et al, 2015) and promoted wound healing by improving scarring outcome and macrophage phenotypes transition (Lei et al, 2018). Punicic acid, a conjugated positional isomer of a-linolenic acid at cis-9, trans-11, and cis-13 positions found in high amounts in pomegranate seed oil, also enhanced transdermal delivery of resveratrol (Liu et al, 2018).…”

Herbal Products in Postsurgical Wound Healing – Incision, Excision and Dead Space Wound Models

“…Some research suggests that the M1 to M2 macrophage phenotype transition is a pivotal step in the successful healing process [86]. Reportedly, rosehip oil promoted wound healing by facilitating this transition [66]. Moreover, rosehip oil increased collagen III content in wound tissue and inhibited epithelial-mesenchymal transition during wound healing to improve scars.…”