Roseomonas alkaliterrae sp. nov., isolated from an alkali geothermal soil sample in Tengchong, Yunnan, south-west China

Abstract

An alkalitolerant, thermotolerant and Gram-stain negative bacterium, designated strain YIM 78007(T), was isolated from an alkaline geothermal soil sample from Hehua hot spring, Tengchong, Yunnan province, south-west China. Cells of strain YIM 78007(T) were observed to be aerobic and short rod-shaped. The colonies were observed to be orange-red, convex and circular. 16S rRNA gene sequence-based phylogenetic analysis showed that strain YIM 78007(T) clustered with members of the genus Roseomonas (with similarities from 97.2 to 92.2 %). Optimal growth of strain YIM 78007 occurs at 40-50 °C and pH 8.0-10.0. The predominant ubiquinone was identified as Q-10 and the major fatty acids were identified as C18:1 ω7c and C16:0. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminolipids and one unknown phospholipid. The G + C content of the genomic DNA was determined to be 63 mol %. The levels of DNA-DNA hybridization relatedness between strain YIM 78007(T) and its closet neighbours (Roseomonas lacus JCM 13283(T) and Roseomonas terrae JCM 14592(T)) were well below the threshold required for the proposal of a novel species. The results of physiological and biochemical characteristics, the phylogenetic analysis, as well as low DNA-DNA hybridization values, allowed the phenotypic and genotypic differentiation of strain YIM 78007(T) from its closest phylogenetic neighbours. Therefore, strain YIM 78007(T) is considered to represent a novel species of the genus Roseomonas, for which the name Roseomonas alkaliterrae sp. nov. is proposed. The type strain is YIM 78007(T) (=BCRC 80644(T) = JCM 19656(T)).

Roseomonas elaeocarpi sp. nov., isolated from olive (Elaeocarpus hygrophilus Kurz.) phyllosphere

“…Rihs et al (1993) reported that the DNA G+C content of the genus Roseomonas ranges from 65 to 71 mol%. However, Kim et al (2013) showed the DNA G+C content of R. aerophila was 73.0 mol%, slightly above values previously reported, and Dong et al (2014) reported a 63.0 mol% DNA G+C content for R. alkaliterrae, a value that was slightly below values for species in the genus Roseomonas. From a literature review, the highest content for a IP: 54.202.233.140…”

“…gilardii KACC 11652 T (Dong et al, 2014) and other members of the genus Roseomonas (Gallego et al, 2006). The level of DNA-DNA relatedness between strain PN2 T and the closest related species, R. mucosa ATCC BAA-692 T , was 27 %, which is well below the 70 % cut-off point for recognition of genomic species (Wayne et al, 1987).…”

“…On: Sat, 12 May 2018 03:08:17 major fatty acid of this genus was for C 18 : 1 v7c under the conditions studied (Gallego et al, 2006;Yoon et al, 2007;Furuhata et al, 2008Furuhata et al, , 2013Yoo et al, 2008;Zhang et al, 2008;Venkata Ramana et al, 2010;Lopes et al, 2011;Kim et al, 2013;Nutaratat et al, 2013;Tonouchi & Tazawa, 2014;Chen et al, 2014;Dong et al, 2014;He et al, 2014, Kim & Ka, 2014 except Roseomonas lacus TH-G33 T , which had iso-C 16 : 0 as the predominant fatty acid (Jiang et al, 2006). Cells contained ubiquinone 10 (Q-10) as the major quinone (Kim et al, 2013;Dong et al, 2014;Chen et al, 2014).…”

“…Based on 16S rRNA gene sequence analysis, this genus belongs to the Alphaproteobacteria (Weyant & Whitney, 2005). At the time of writing, 23 species and two subspecies with validly published names are members of the genus Roseomonas, recently recognized species including Roseomonas aerophila (Kim et al, 2013), 'Roseomonas musae' (Nutaratat et al, 2013), Roseomonas tokyonensis (Furuhata et al, 2013), 'Roseomonas aceris' (Tonouchi & Tazawa, 2014), Roseomonas alkaliterrae (Dong et al, 2014), Roseomonas rhizosphaerae (Chen et al, 2014), 'Roseomonas sediminicola' (He et al, 2014) and Roseomonas soli (Kim & Ka, 2014). There have been reports on the isolation of Roseomonas species from various sources such as medical and environmental samples including plant phyllosphere.…”

“…Cells contained ubiquinone 10 (Q-10) as the major quinone (Kim et al, 2013;Dong et al, 2014;Chen et al, 2014). With regard to the polyamine pattern, members of the genus Roseomonas have spermidine as the predominant compound (Sánchez-Porro et al, 2009).…”