Roseovarius faecimaris sp. nov., isolated from a tidal flat

Cha, In-Tae; Cho, Eui-Sang; Choi, Hak-Jong; Roh, Seong Woon; Seo, Myung-Ji

doi:10.1099/ijsem.0.003345

“…S2). Species of the two genera Roseovarius and Pelagicola appeared adjacent to each other in the 16S phylogenetic tree, this phenomenon was also found in other studies [20][21][22], which may mean that their evolutionary status is close. However, the two genera can still be separated based on the results of 16S phylogenetic tree analysis.…”

supporting

confidence: 83%

Roseovarius arcticus sp. nov., a bacterium isolated from Arctic marine sediment

Lu

¹

,

Peng

²

,

Liu

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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An aerobic, Gram-stain-negative, motile, rod or long-rod-shaped bacterial isolate, strain MK6-18T, was isolated from a marine sediment sample from Kongsfjorden, Arctic. The bacterium grew optimally at 20 °C, pH 7.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MK6-18T belonged to the genus Roseovarius . Its closest phylogenetic neighbour was Roseovarius nanhaiticus NH52JT showing 96.97 % 16S rRNA gene sequence similarity. The genome of strain MK6-18T is 4.2 Mb long in size with a G+C content of 59.5 mol%. The average nucleotide identity value between the genomes of strain MK6-18T and Roseovarius nanhaiticus NH52JT, was 78.0 %. Similar to other species of the genus Roseovarius , strain MK6-18T had ubiquinone 10 as the predominant ubiquinone and C12 : 0, C16 : 0, summed feature 3 (C16 : 1ω7c/ω6c) and summed feature 8 (C18 : 1ω7c/ω6c) as the major fatty acids. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine; one unidentified polar lipid, one unidentified aminolipid and one unidentified lipid were also detected. This is the first time that a member of the genus Roseovarius has been isolated from the Arctic, which may promote the study of the distribution characteristics and environmental adaptability of this genus. On the basis of the data provided here, strain MK6-18T should be classed as representing a novel species of the genus Roseovarius , for which the name Roseovarius arcticus sp. nov. is proposed. The type strain is MK6-18T (=CCTCC AB 2018219T=KCTC 72187T).

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“…The major polar lipids phosphatidylcholine and phosphatidylglycerol were present in strain HL-MP18 T and the related Roseovarius species (Table 1), a feature that is generally found in the genus Roseovarius [67]. The isoprenoid quinone of strain HL-MP18 T was ubiquinone-10, which is also common in the genus Roseovarius [1,4,[8][9][10][11][12][13][14][25][26][27][28][29][30][31] In conclusion, the phylogenetic, genomic, phenotypic and chemotaxonomic data obtained in the present study suggest that strain HL-MP18 T should be placed in the genus Roseovarius as a representative of a novel species, for which the name Roseovarius pelagicus is proposed.…”

Section: Chemotaxonomymentioning

confidence: 89%

“…The major polar lipids phosphatidylcholine and phosphatidylglycerol were present in strain HL-MP18 T and the related Roseovarius species (), a feature that is generally found in the genus Roseovarius [67]. The isoprenoid quinone of strain HL-MP18 T was ubiquinone-10, which is also common in the genus Roseovarius [1, 4, 8–14, 25–31]…”

Section: Chemotaxonomymentioning

confidence: 99%

“…However, the detection of bacterial sequences belonging to this genus in PP microplastics suggests their potential association with PP degradation in marine environments [6]. Most members of the genus Roseovarius have been isolated from marine environments, such as seawater [4,[7][8][9][10][11][12][13][14], sediment [15][16][17][18][19][20][21][22][23][24], soil [25,26], decayed whale bone [27], sponge [28], shell [29] and dinoflagellates [30,31]. In this study, we describe the characteristics of a marine bacterium, designated HL-MP18 T , using a polyphasic approach to identify its taxonomic position.…”

Section: Introductionmentioning

confidence: 99%

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Roseovarius pelagicus sp. nov., a facultatively anaerobic bacterium with potential for degrading polypropylene, isolated from Arctic seawater

Hong

¹

,

Jang

²

,

Kim

³

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

1

0

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A Gram-stain-negative, facultatively anaerobic, non-motile, rod-shaped bacterial strain, designated HL-MP18T, was isolated from Arctic seawater after a prolonged incubation employing polypropylene as the sole carbon source. Phylogenetic analyses of the 16S rRNA gene sequence revealed that strain HL-MP18T was affiliated to the genus Roseovarius with close relatives Roseovarius carneus LXJ103T (96.8 %) and Roseovarius litorisediminis KCTC 32327T (96.5 %). The complete genome sequence of strain HL-MP18T comprised a circular chromosome of 3.86 Mbp and two circular plasmids of 0.17 and 0.24 Mbp. Genomic comparisons based on average nucleotide identity and digital DNA–DNA hybridization showed that strain HL-MP18T was consistently discriminated from its closely related taxa in the genus Roseovarius . Strain HL-MP18T showed optimal growth at 25 °C, pH 7.0 and 2.5 % (w/v) sea salts. The major cellular fatty acids were C18 : 1 ω6c and/or C18 : 1 ω7c (49.6 %), C19 : 0 cyclo ω8c (13.5 %), and C16 : 0 (12.8 %). The major respiratory quinone was ubiquinone-10. The polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and three unidentified lipids. The genomic DNA G+C content of the strain was 59.2 mol%. The phylogenetic, genomic, phenotypic and chemotaxonomic results indicate that strain HL-MP18T is distinguishable from the recognized species of the genus Roseovarius . Therefore, we propose that strain HL-MP18T represents a novel species belonging to the genus Roseovarius , for which the name Roseovarius pelagicus sp. nov. is proposed. The type strain is HL-MP18T (=KCCM 90405T=JCM 35639T).

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“…At the time of writing, 26 species of the genus Roseovarius with validly published names have been described (www.bacterio.net/roseovarius.html). In addition, the species Roseovarius faecimaris [8], Roseovarius ponticola [9] and Roseovarius amoyensis [10] have recently been proposed. Members of the genus Roseovarius contain Q-10 as the predominant respiratory quinone and C 18 : 1 ω6 c and/or C 18 : 1 ω7 c as the dominant cellular fatty acids.…”

Section: Full-textmentioning

confidence: 99%

Roseovarius gahaiensis sp. nov., isolated from Gahai Saline Lake, PR China

Sun¹,

Wang

²

,

Liu

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

5

0

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A Gram-stain-negative, aerobic, non-motile and ovoid- to rod-shaped bacterial strain, designated GH877T, was isolated from a water sample of Gahai saline lake in Qaidam Basin,PR China. The isolate grew at 5–45 °C, pH 6.0–9.0 (optima, 37 °C and pH 7.5) and with 0.5–20 % (w/v) NaCl (optimum, 2.0 %). The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain GH877T belonged to the genus Roseovarius , and had highest 16S rRNA gene sequence similarity of 97.7 % to Roseovarius pacificus 81-2T, followed by Roseovarius halotolerans HJ50T (97.5 %) and Roseovarius litoreus GSW-M15T (96.8 %). Genome sequencing revealed a genome size of 3 378 519 bp and a G+C content of 59.8 mol %. Up-to-date bacterial core gene set analysis indicated that strain GH877T represents one independent lineage with R. pacificus DSM29589T. The average nucleotide identity values of GH877T with R. pacificus 81-2T and R. halotolerans HJ50T are 80.7 and 77.3 %, respectively. In silico DNA–DNA hybridization values between strain GH877T and R. pacificus 81-2T and R. halotolerans HJ50T are 23.2 and 20.0 %, respectively. Q-10 was the predominant respiratory quinone and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C16 : 0 were the major cellular fatty acids. The polar lipids of strain GH877T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified phosphoglycolipids. Based on polyphasic taxonomic analysis, strain GH877T is proposed to represent a novel species of the genus Roseovarius , for which the name Roseovarius gahaiensis sp. nov. is proposed. (type strain GH877T=CGMCC 1.13971T=KCTC 72576T).

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Roseovarius faecimaris sp. nov., isolated from a tidal flat

Cited by 8 publications

References 36 publications

Roseovarius arcticus sp. nov., a bacterium isolated from Arctic marine sediment

Roseovarius arcticus sp. nov., a bacterium isolated from Arctic marine sediment

Roseovarius pelagicus sp. nov., a facultatively anaerobic bacterium with potential for degrading polypropylene, isolated from Arctic seawater

Roseovarius gahaiensis sp. nov., isolated from Gahai Saline Lake, PR China

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