Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi

Abstract

A Gram-negative, motile, ovoid-to rod-shaped bacterial strain, designated MA1-10 T , was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, Korea. Strain T grew optimally at pH 7.0-8.0, at 30 6C and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain MA1-10 T clustered with Roseovarius crassostreae CV919-312 T , with which it exhibited 97.1 % sequence similarity, at a bootstrap resampling value of 96.2 %. It exhibited 93.3-95.8 % 16S rRNA gene sequence similarity to the type strains of other recognized Roseovarius species. Strain MA1-10 T contained Q-10 as the predominant ubiquinone and C 18 : 1 v7c as the major fatty acid, which is consistent with data for the genus Roseovarius. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid. The DNA G+C content of strain MA1-10 T was 55.4 mol%. Mean DNA-DNA relatedness between strain MA1-10 T and R. crassostreae DSM 16950 T was 13 %.Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain MA1-10 T could be distinguished from all recognized Roseovarius species. On the basis of the data presented, strain MA1-10

Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment

“…Stappia stellulata IAM 12621 T (D88525) related genera by not having phosphatidylethanolamine as a major component (Yoon et al, 2009(Yoon et al, , 2013aKim et al, 2012;Lucena et al, 2012Lucena et al, , 2013Park et al, 2012; Table S1). The G+C content of strain BS-B2 T was 62.2 mol%, a value similar to those of the type strains of P. sedentarius and M. geojedonensis (Table 1).…”

Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

“…The genus Roseovarius accommodates catalase-positive, ovoid or rodshaped, obligately aerobic bacteria and, to date, all Roseovarius type strains have been retrieved from marine environments such as tidal flats, seawater, sea squirts and oysters. At the time of writing, the genus Roseovarius included 13 species with validly published names: R. tolerans (Labrenz et al, 1999), R. nubinhibens (González et al, 2003), R. mucosus (Biebl et al, 2005), R. crassostreae (Boettcher et al, 2005), R. aestuarii (Yoon et al, 2008), R. halotolerans (Oh et al, 2009), R. pacificus (Wang et al, 2009), R. nanhaiticus (Wang et al, 2010), R. indicus (Lai et al, 2011), R. marinus (Jung et al, 2011), R. halocynthiae (Kim et al, 2012b), R. litoreus (Jung et al, 2012) and R. sediminilitoris (Park & Yoon, 2013). Tidal flats, called getbol in Korean, are broad, low-gradient, coastal muddy marshes that play important roles in habitat restoration and nutrient recycling (Winberg et al, 2007;Math et al, 2012).…”

Roseovarius sediminilitoris sp. nov., isolated from seashore sediment

“…The major fatty acids (.10 % of the total fatty acids) found in strain M-M10 T were C 18 : 1 v7c and C 16 : 0 in line with those of other species of the genus Roseovarius and the majority members of the Alphaproteobacteria. The fatty acid profile of strain M-M10 T was essentially similar to those of species of the genus Roseovarius in that the predominant fatty acid is C 18 : 1 v7c, although there were differences in the proportions of some fatty acids (Table S1; Kim et al, 2012). Polar lipids were extracted according to the procedures described by Minnikin et al (1984) and separated by two-dimensional TLC using chloroform : -methanol : water (65 : 25 : 3.8, by volume) for the first dimension and chloroform : methanol : acetic acid : water (40 : 7.5 : 6:1.8, by volume) for the second dimension as described by Minnikin et al (1977).…”

“…At the time of writing, the genus Roseovarius comprises 11 species with validly published names (Euzéby, 1997). Members of the genus Roseovarius have been isolated from a hypersaline lake, seawater, a dinoflagellate culture, oysters, marine sediments and marine organisms (Labrenz et al, 1999;González et al, 2003;Biebl et al, 2005;Boettcher et al, 2005;Yoon et al, 2008;Wang et al, 2009;Kim et al, 2012). In this study, we describe a bacterial strain, designated M-M10 T resembling members of the genus Roseovarius, which was isolated from seashore sediment collected from Geoje island of the South Sea, South Korea.…”

“…The almost complete 16S rRNA gene sequence of strain M-M10 T determined in this Strains: 1, Roseovarius sediminilitoris M-M10 T ; 2, Roseovarius crassostreae DSM 16950 T ; 3, Roseovarius halocynthiae MA1-10 T ; 4, Roseovarius marinus HDW-9 T ; 5, Roseovarius tolerans DSM 11457 T . Data for the reference strains are taken from Yoon et al (2008), Jung et al (2011) and Kim et al (2012) unless otherwise stated. +, Positive reaction; 2, negative reaction; W, weakly positive reaction.…”