Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula

Jung, Yong-Taek; Park, Sooyeon; Yoon, Jung‐Hoon

doi:10.1007/s10482-012-9721-3

Cited by 29 publications

(12 citation statements)

References 31 publications

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“…The genus Roseovarius accommodates catalase-positive, ovoid or rodshaped, obligately aerobic bacteria and, to date, all Roseovarius type strains have been retrieved from marine environments such as tidal flats, seawater, sea squirts and oysters. At the time of writing, the genus Roseovarius included 13 species with validly published names: R. tolerans (Labrenz et al, 1999), R. nubinhibens (González et al, 2003), R. mucosus (Biebl et al, 2005), R. crassostreae (Boettcher et al, 2005), R. aestuarii (Yoon et al, 2008), R. halotolerans (Oh et al, 2009), R. pacificus (Wang et al, 2009), R. nanhaiticus (Wang et al, 2010), R. indicus (Lai et al, 2011), R. marinus (Jung et al, 2011), R. halocynthiae (Kim et al, 2012b), R. litoreus (Jung et al, 2012) and R. sediminilitoris (Park & Yoon, 2013). Tidal flats, called getbol in Korean, are broad, low-gradient, coastal muddy marshes that play important roles in habitat restoration and nutrient recycling (Winberg et al, 2007;Math et al, 2012).…”

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Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

Choi

Lee

Kim

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-staining-negative, moderately halophilic and strictly aerobic bacterium, designated strain 112 T , was isolated from a tidal flat at Taean, Korea. Cells were catalase-and oxidase-positive ovoids to rods and did not produce bacteriochlorophyll a. Optimum growth of strain 112T was observed at 30 6C, at pH 6.5-7.5 and in the presence of 2-4 % (w/v) NaCl.The major cellular fatty acids were summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c) and C 16 : 0 and Q-10 was detected as the predominant ubiquinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and three unidentified lipids. Math et al., 2012). In this study, we describe the taxonomic characterization of a novelRoseovarius strain, designated 112 T , isolated from a tidal flat of the Yellow Sea in South Korea. Strain 112T was isolated from a surface tidal flat (less than 5 cm depth) in the Taean area (36 u 489 50.820 N 126 u 119 09.560 E), using a previously described procedure (Jin et al., 2011) with some modifications. Briefly, the tidal flat sample was serially diluted with artificial seawater (ASW; Jeong et al., 2013), spread on marine agar 2216 (MA; BD) and incubated at 25 u C for 3 days. The 16S rRNA genes of isolates that grew on MA were amplified by PCR using the universal primers F1 and R13 (Jin et al., 2011). The amplicons were double-digested with HaeIII and HhaI and the restriction fragment patterns were used as a guide to classify the colonies, as described previously (Jin et al., 2012). Based on restriction fragment patterns, PCR products with unique patterns were selected and sequenced using the F1 primer. The resulting 16S rRNA gene sequences were analysed using the Nucleotide Similarity Search program (http://eztaxon-e.ezbiocloud.net/; Kim et al., 2012a). From this analysis, a novel strain belonging to the genus Roseovarius, designated strain 112 T , was selected for further phenotypic and phylogenetic analysis. Strain 112T was routinely grown aerobically on MA at 30 u C for 2 days, except where indicated otherwise. Strain 112T was stored at 280 u C in marine broth (MB; BD) supplemented with 10 % (v/v) glycerol. The type strains R. mucosus KACC 12996 T , R. tolerans KACC 13027 T and R. nanhaiticus KACC 12561T were used as reference strains for phenotypic comparisons and fatty acid analysis.Abbreviation: BChl, bacteriochlorophyll.

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Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

Choi

Lee

Kim

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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“…Motility was observed using the semi-solid agar media [54]. Strain JB22 T was cultivated at various temperatures (5,10,15,20,25,28,30,37, and 45°C) to measure optimal temperature and temperature range for growth. The pH range for growth was determined in marine broth adjusted 3.0-10.0 (at increments of 1 pH units) by using sodium acetate buffer and Tris-HCl buffer.…”

Section: Phenotypic and Biochemical Characterizationmentioning

confidence: 99%

Nitropelagi marinus gen. nov., sp. nov., Isolated From Seawater, Je-bu island, South Korea

Jeong

Lee

2016

Curr Microbiol

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A Gram-stain-negative, non-spore forming, non-motile and aerobic strain, designated JB22(T), was isolated from seawater, Je-bu Island, South Korea. Strain JB22(T) was catalase and oxidase positive. Optimal growth of JB22(T) was observed at 30 °C and pH 7.0. NaCl tolerance range was 1-9 % (w/v) with an optimum of 2.0 % concentration. The phylogenetic analysis based on 16S rRNA gene sequence of strain JB22(T) showed the highest sequence similarity to those of Pelagicola litorisediminis D1-W8(T) (95.8 %), Roseovarius litoreus GSW-M15(T) (95.2 %), Roseovarius aestuarii SMK-122(T) (95.0 %), Donghicola eburmeus SW-277(T) (95.0 %), and Roseovarius halotolerans HJ50(T) (94.9 %). It contained ubiquine-10 as the major respiratory quinone and C18:1 ω7c (69.3 %), :0 (9.9 %), C18:1 ω7c 11-methyl (9.6 %) as the major fatty acid. The polar lipid profile included phosphatidylcholine, phosphatidylglycerol, and unidentified aminolipid. The DNA G+C content of the strain JB22(T) was 47 mol %. Based on physiological and chemotaxonomic characteristics, strain JB22(T) should be regarded as a new genus of the family Rhodobacteraceae, for which the Nitropelagi marinus gen. nov., sp. nov. is proposed. The type strain is JB22(T) (= KEMB 3001-101(T) = JCM 30822(T)).

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“…Representative bacteria of the genus Roseovarius are Gram-stain-negative, aerobic, ovoid or rodshaped, bacteriochlorophyll a-positive, oxidase and catalase positive and require Na + for growth. Most of the bacteria of this genus have been isolated from marine environments including tidal flat, hypersaline lake, deep-sea sediment or seawater [2][3][4][5][6][7][8]. In general, the major polar lipids representative of this genus are phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol, and the major fatty acids are C 18 : 1 ω7c/C 18 : 1 ω6c, C 16 : 0 and 11-methyl-C 18 : 1 ω7c.…”

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Roseovarius bejariae sp. nov., a moderately halophilic bacterium isolated from a hypersaline steep-sided river bed

Castro¹,

Gomez-Altuve²,

Reina³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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An aerobic, Gram-stain-negative ovoid, designated as strain A21T, was isolated using the dilution-to-extinction method from a soil sample taken from Rambla Salada, an athalassohaline habitat located in Murcia (south-eastern Spain). Strain A21T is non-motile, has a respiratory metabolism and grows at NaCl concentrations within the range 0.5–15 % (w/v) [optimum, 5 % (w/v)], at 5–35 °C (optimum, 28 °C) and at pH 6–8 (optimum, pH 7.0). This strain is positive for catalase activity, oxidase activity and nitrate reduction. The 16S rRNA gene sequence indicates that it belongs to the genus Roseovarius in the class Alphaproteobacteria . The most closely related species are Roseovarius pacificus and Roseovarius halotolerans to which the strain A21T shows 16S rRNA gene sequence similarity values of 98.06 and 97.7 %, respectively. The average nucleotide identity in blast and digital DNA–DNA hybridization values between strain A21T and R. pacificus LMG 24575T are 76.8 and 21 %, respectively. The DNA G+C content based on the genome is 61.28 mol%. The major fatty acids (>5 % of the total fatty acids) of strain A21T are C18 : 1 ω7c/C18 : 1 ω6c and C16 : 0. The only detected isoprenoid quinone in strain A21T is ubiquinone 10 (Q-10). The polar lipid profile contains phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and three unidentified polar lipids. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the strain represents a novel species of the genus Roseovarius , for which the name Roseovarius bejariae sp. nov. is proposed. Strain A21T (=CECT 9817T=LMG 31311T) is the type strain.

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Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula

Cited by 29 publications

References 31 publications

Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

Nitropelagi marinus gen. nov., sp. nov., Isolated From Seawater, Je-bu island, South Korea

Roseovarius bejariae sp. nov., a moderately halophilic bacterium isolated from a hypersaline steep-sided river bed

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