Roseovarius nanhaiticus sp. nov., a member of the Roseobacter clade isolated from marine sediment

Abstract

The genus Roseovarius accommodates Gram-negative, ovoid or rod-shaped, obligate aerobic alphaproteobacteria and all its members are positive in tests for catalase. All of the species were retrieved from marine environments and belong to the Roseobacter group. Many novel representatives of this group have been isolated from marine habitats all over the world and described recently (Wagner-Döbler et al., 2003;Buchan et al., 2005;Dai et al., 2006;Lee et al., 2007;Hwang & Cho, 2008). In this paper, a novel Gram-negative, beige-to slightly pink-pigmented, aerobic bacterium is described that was isolated from sediment of the South China Sea. By using 16S rRNA gene sequence phylogenetic analysis, the new strain was determined to be a member of the Roseobacter group, in which it formed a distinct lineage with members of the genus Roseovarius. Based on differences in fatty acid composition, physiological and biochemical characteristics, morphological features, and phylogenetic analysis as described in this study, the new strain should be classified as a novel species of the genus Roseovarius.Recently, bacterial diversity investigations have led to the discovery of many novel species and genera from the South China Sea. One of these strains, designated NH52JT , was isolated from a brown sandy sediment sample (109 u 40.1029 E, 06 u 5.9019 N), which was collected at water depth of 157 m in June 2007. In the laboratory, the sediment sample was suspended and diluted (1 : 100) using sterilized seawater, spread onto M2 agar plates and then incubated at 25 u C for 1 week. The composition of M2 medium was (l 21 seawater): 5 g CH 3 COONa, 0.5 g peptone, 0.5 g yeast extract, 0.5 g glucose, 0.5 g sucrose, 0.5 g starch, 0.05 g trisodium citrate, 0.05 g malic acid, 0.05 g potassium sodium tartrate, 1.0 g NH 4 NO 3 , 0.2 g NH 4 Cl and 15 g agar, adjusted to pH 7.5-7.6. Strain NH52JT was picked and streaked on fresh M2 agar medium. This process was repeated three times for purification and the strain was preserved as a 20 % (v/v) glycerol suspension at 280 u C. Cell morphology and motility were observed by phase-contrast microscopy Abbreviation: Bchl a, bacteriochlorophyll a.3These authors contributed equally to this work.The GenBank accession number for the 16S rRNA gene sequence of Roseovarius nanhaiticus sp. nov. NH52JT is FJ403243.

Roseovarius lutimaris sp. nov., isolated from a marine tidal flat

“…The genomic DNA G+C content of strain 112 T was 58.2 mol%, which was within the range reported for other type strains of the genus Roseovarius (Wang et al, 2010;Kim et al, 2012b). The major polar lipids detected in strain 112…”

“…The genus Roseovarius accommodates catalase-positive, ovoid or rodshaped, obligately aerobic bacteria and, to date, all Roseovarius type strains have been retrieved from marine environments such as tidal flats, seawater, sea squirts and oysters. At the time of writing, the genus Roseovarius included 13 species with validly published names: R. tolerans (Labrenz et al, 1999), R. nubinhibens (González et al, 2003), R. mucosus (Biebl et al, 2005), R. crassostreae (Boettcher et al, 2005), R. aestuarii (Yoon et al, 2008), R. halotolerans (Oh et al, 2009), R. pacificus (Wang et al, 2009), R. nanhaiticus (Wang et al, 2010), R. indicus (Lai et al, 2011), R. marinus (Jung et al, 2011), R. halocynthiae (Kim et al, 2012b), R. litoreus (Jung et al, 2012) and R. sediminilitoris (Park & Yoon, 2013). Tidal flats, called getbol in Korean, are broad, low-gradient, coastal muddy marshes that play important roles in habitat restoration and nutrient recycling (Winberg et al, 2007;Math et al, 2012).…”

Roseovarius litoreus sp. nov., isolated from seawater of southern coast of Korean peninsula

“…This profile was similar with that of the type strain of R. tolerans shown by Kim et al (2011) in that phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids exist as major polar lipids, although diphosphatidylglycerol, which is major polar lipid in the type strain of R. tolerans, was only detected as a minor component in strain GSW-M15 T . The DNA G?C content of strain GSW-M15 T was 62.9 mol%, a value in the range reported for Roseovarius species (Wang et al 2009(Wang et al , 2010Jung et al 2011;Lai et al 2011).…”

“…Subsequently, nine further Roseovarius species with validly published names, Roseovarius nubinhibens (González et al 2003), Roseovarius crassostreae (Boettcher et al 2005), Roseovarius mucosus (Biebl et al 2005), Roseovarius aestuarii (Yoon et al 2008), Roseovarius pacificus (Wang et al 2009(Wang et al , 2010, Roseovarius halotolerans (Oh et al 2009), Roseovarius nanhaiticus (Wang et al 2010), Roseovarius marinus (Jung et al 2011) and Roseovarius indicus (Lai et al 2011), have been described from a hypersaline lake, seawater, a dinoflagellate culture, oysters and marine sediments (Labrenz et al 1999;González et al 2003;Biebl et al 2005;Boettcher et al 2005;Yoon et al 2008;Wang et al 2009Wang et al , 2010. In this study, we describe a bacterial strain, designated GSW-M15 T , which was isolated from seawater on the southern coast of South Korea and found to be phylogenetically affiliated to the genera Roseovarius and Donghicola on the basis of comparative 16S rRNA gene sequence analysis.…”

Roseovarius atlanticus sp. nov., isolated from surface seawater

“…(Biebl et al, 2005); Roseovarius nanhaiticus was isolated from a sandy sediment sample from the South China Sea (Wang et al, 2010); Roseovarius pacificus was isolated from deep-sea sediment of the Western Pacific Ocean (Wang et al, 2009); and Roseovarius lutimaris was isolated from a marine tidal flat (Choi et al, 2013). In addition, three species (Roseovarius crassostreae, Roseovarius halocynthiae and Roseovarius sediminilitoris) of the genus Roseovarius have recently been reclassified into the genus Aliiroseovarius (Park et al, 2015), and the species Roseovarius marinus has been reclassified as 'Pacificibacter marinus' (Park et al, 2014a).…”