Rosiglitazone enhances neovascularization in diabetic rat ischemic hindlimb model

Khazaei, Majid; Salehi, Ensieh

doi:10.5507/bp.2012.052

Cited by 3 publications

(4 citation statements)

References 33 publications

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“…Therapeutic angiogenesis and arteriogenesis remain an important goal in the case of patients with critical ischemia without revascularization possibilities. Many therapies have been attempted, with more or less encouraging effects [ 2 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…Capillary density (number of capillaries per number of muscle fibers) was determined in 10 different fields (magnification ×400), from each section from each animal, and was expressed as the mean number of CD31 positive cells per mm 2 . To avoid underestimation or overestimation of muscle density due to muscle atrophy or interstitial edema, the capillary/muscle fiber ratio was also expressed [ 17 ] (Khazaei and Salehi, 2012).…”

Section: Methodsmentioning

confidence: 99%

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Treatment with Sildenafil and Donepezil Improves Angiogenesis in Experimentally Induced Critical Limb Ischemia

Constantinescu

Bolfă

Mironiuc

et al. 2017

BioMed Research International

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Objectives. In this study, we aimed to demonstrate the role of sildenafil (an antagonist of phosphodiesterase type 5 (PDE-5)) and donepezil (a specific and reversible inhibitor of acetylcholinesterase (Ach)) in increasing ischemia-induced angiogenesis. Method. Critical limb ischemia was induced by ligation of the common femoral artery followed by ligation of the common iliac artery. The operated animals were divided into 3 groups: receiving sildenafil, receiving donepezil, and surgery alone; the contralateral lower limb was used as a negative control. The results were controlled based on clinical score and Doppler ultrasound. Gastrocnemius muscle samples were taken from all animals, both from the ischemic and nonischemic limb and were used for histopathological and immunohistochemical examination for the evaluation of the number of nuclei/field, endothelial cells (CD31), dividing cells (Ki-67), and vascular endothelial growth factor (VEGFR-3). Results. An increasing tendency of the number of nuclei/field with time was observed both in the case of sildenafil and donepezil treatment. The formation of new capillaries (the angiogenesis process) was more strongly influenced by donepezil treatment compared to sildenafil or no treatment. This treatment significantly influenced the capillary/fiber ratio, which was increased compared to untreated ligated animals. Sildenafil treatment led to a gradual increase in the number of dividing cells, which was significantly compared to the negative control group and compared to the ligation control group. The same effect (increase in the number of Ki-67 positive cells) was more obvious in the case of donepezil treatment. Conclusion. Donepezil treatment has a better effect in ligation-induced ischemia compared to sildenafil, promoting angiogenesis in the first place, and also arteriogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Treatment with Sildenafil and Donepezil Improves Angiogenesis in Experimentally Induced Critical Limb Ischemia

Constantinescu

Bolfă

Mironiuc

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…), VEGF‐C (Kuwahara et al . ) and rosiglitazone (Khazaei & Salehi ), which enhance capillary density and improve blood distribution after femoral artery ligation. Lu et al .…”

Section: Common Rodent Models Of Ischaemia or Non‐ischaemic Hypoperfumentioning

confidence: 99%

“…Ligation of the femoral artery leads, depending on the ligation site, to mild or severe ischaemia in the leg. Recently identified factors of therapeutic potential include prostacyclin (Aburakawa et al 2012), cilostazol (Biscetti et al 2012), VEGF-C (Kuwahara et al 2012) and rosiglitazone (Khazaei & Salehi 2012), which enhance capillary density and improve blood distribution after femoral artery ligation. Lu et al identified a new angiogenic factor AGGF1 (angiogenic factor with G patch and FHA domain-1), which triggers angiogenesis in this model (Lu et al 2012).…”

mentioning

confidence: 99%