Rosmarinic acid content and RAPD analysis of in vitro regenerated basil (Ocimum americanum) plants

Rady, M. R.; Nazif, Naglaa M.

doi:10.1016/j.fitote.2005.04.001

Cited by 37 publications

(25 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Somaclonal variations, have been detected in DNA fingerprint analysis of Ocimum americanum (Rady and Nazif 2005) and Musa paradisca (El-Dougdoug et al 2007). Genetic stability of in vitro regenerated plants of Ajuga bracteosa were assessed by RAPD.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Ajuga bracteosa, a medicinal herb

Kaul

Das

Srivastava

2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

For conservation and genetic transformation, a successful in vitro micropropagation protocol for Ajuga bracteosa, a medicinal herb has been established for the first time. MS medium supplemented with IAA (2 mg/L) and BA (5 mg/L) induced 100 % shoot regeneration with an average of 41.4 shoots of 8.4 cm per culture. Excised in vitro shoots when transferred to MS + IBA (0.5 mg/L) produced 20 roots/shoot of 20.2 cm average length in 100 % cultures. Of the three explants, leaf, petiole and root, leaf displayed quickest response followed by petiole while root was the slowest. Hardening of plantlets was achieved with 82 % survival. The hardened plants were maintained in pots with garden soil under controlled (Temp. 25±2°C) conditions. RAPD exhibited genetic fidelity with 100 % monomorphism in regenerants.

show abstract

Section: Discussionmentioning

confidence: 99%

Micropropagation of Ajuga bracteosa, a medicinal herb

Kaul

Das

Srivastava

2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…4a) and rosmarinic acid (Fig. 4b) by hairy roots of Salvia miltiorrhiza Bunge, while Rady and Nazif (2005) described the production of rosmarinic acid in shoots of Ocimum americanum L. var pilosum. The aim was to increase the bioactive potential of plants traditionally used and for which the production capacity of these compounds through in vitro culture was unknown until then.…”

Section: Production Of Phenolic Compounds Through Plant Tissue Culturmentioning

confidence: 99%

Exploring plant tissue culture to improve the production of phenolic compounds: A review

Días

Sousa

Alves

et al. 2016

Industrial Crops and Products

238

162

View full text Add to dashboard Cite

Plant tissue and organ culture has been extensively used from the beginning of the XX century for the study and comprehension of some primary biological mechanisms such as morphogenesis. However, with the increasing demand of the market for novel products derived from plants, in vitro culture became a reliable technique for the mass production of plant material. Moreover, the potential to use this technique for the production of some bioactive compounds, such as phenolic compounds, is immense since it allows the manipulation of the biosynthetic routes to increase the production and accumulation of specific compounds. This work intends to make a brief historical review of in vitro culture, highlighting its use for the production of bioactive compounds. Also, emphasizes the importance of phenolic compounds for the consumer as well reviews the metabolic pathways involved in its production in plant cells.Furthermore, it was carried out a comprehensive study on the work developed for the production of plant phenolic compounds in in vitro cultures, as well as on the type of elicitors used to increase of the same production; also a brief highlighting of the phenolic compounds which serve as elicitors. There are numerous reports directed to the production of phenolic extracts in in vitro plant cultures, however there is a lack in the production of individual phenolic compounds mainly due to the complexity of the biosynthetic routes and extraction procedures. Elicitation procedures are often used to increase the production of phenolics, archieving in most cases higher yields than in nonelicitated cultures. The increasing production of bioactive phenolic extracts/compounds allows for their further applicability, namely in the industry of functional foods or in pharmaceutical/medical fields.3

show abstract

“…Increasing BA concentrations resulted in an increase of RA content in green-leaf cultivars, which coincides with previous findings (Figure 4). Rady and Nazif [18] observed that RA content increased in in vitro shoots of Ocimum americanum L. with BA concentrations up to 1 mg L -1 . In contrast to greenleaf basil genotypes, RA content decreased in purpleleaf Dark Opal at BA concentrations above 0.5 mg L -1 .…”

Section: In Vitro Culturementioning

confidence: 99%

“…The possible application of plant tissue culture for the production of bioactive compounds has been demonstrated in several species [9,14], although at present only very few substances are produced in vitro on a commercial scale [15]. Different basil species were found to accumulate larger quantities of RA in cell, callus, hairy roots and shoot cultures than in vivo [16][17][18][19]. The total phenolic and RA levels were higher in sweet basil grown hydroponically than in soil-grown plants [20] Compared to in vitro culture, hydroponics offers the advantages of a higher rate of biomass production per unit area and less expensive growing structures [21,22], although the production cost per unit weight of the metabolites of interest is not necessarily lower in vivo than in vitro [23].…”

Section: Introductionmentioning

confidence: 99%

Rosmarinic acid content in basil plants grown in vitro and in hydroponics

et al. 2011

View full text Add to dashboard Cite

The accumulation of selected caffeic acid derivatives (CADs), in particular rosmarinic acid (RA), was investigated in different tissues (leaves, roots and plantlet shoots) of sweet basil (Ocimum basilicum L.) plants grown either in vitro or in hydroponic culture (floating system) under greenhouse conditions. Two cultivars with green leaves (Genovese and Superbo) and one with purple leaves (Dark Opal) were tested. The content of CADs in HCl-methanol extracts was determined by HPLC. LC-MS and LC-MS-MS were used to confirm the identification of the metabolites of interest. Apart from rosmarinic acid (RA) and a methylated form of this substance, no other CADs were detected at significant level in any of the analyzed samples. The content of RA ranged approximately from 4 to 63 mg/g DW, depending on the growing system. The highest RA content was found during the in vitro multiplication, in the acclimatized plants and in the roots of hydroponically-grown seedlings at full bloom. In vitro, 6-benzyladenine reduced the accumulation of RA in purple-leaf Dark Opal cultivar, but an opposite effect of this growth regulator was observed in the green-leaf genotypes. Our findings suggest the possibility to scale-up RA production by means of in vitro or hydroponic culture of sweet basil.

show abstract

Rosmarinic acid content and RAPD analysis of in vitro regenerated basil (Ocimum americanum) plants

Cited by 37 publications

References 20 publications

Micropropagation of Ajuga bracteosa, a medicinal herb

Micropropagation of Ajuga bracteosa, a medicinal herb

Exploring plant tissue culture to improve the production of phenolic compounds: A review

Rosmarinic acid content in basil plants grown in vitro and in hydroponics

Contact Info

Product

Resources

About