Rosmarinic acid inhibits the formation of reactive oxygen and nitrogen species in RAW264.7 macrophages

Qiao, Shanlou; Li, Weihua; Tsubouchi, Ryoko; Haneda, Miyako; Murakami, Keiko; Takeuchi, Fumio; Nisimoto, Yukio; Yoshino, Masataka

doi:10.1080/10715760500231836

Cited by 119 publications

(70 citation statements)

References 17 publications

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“…Additional studies have shown that RA eff ectively inhibited ROS-mediated damage in astrocytes and macrophages (Gao et al, 2005;Qiao et al, 2005). Furthermore, other studies suggested that RA may prevent apoptosis in cardiac muscle cells (Kim et al, 2005), PC12 cells (Iuvone et al, 2006), and human dopaminergic neuronal cells (Lee et al, 2008).…”

Section: Discussionmentioning

confidence: 95%

Protective effect of rosmarinic acid on V79 cells evaluated by the micronucleus and comet assays

Furtado

Araújo

Resende

et al. 2009

J of Applied Toxicology

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Rosmarinic acid (RA) is a naturally occurring phenolic compound, which contributes to the beneficial and health-promoting effects of herbs, spices and medicinal plants. RA has shown several biological activities, such as hepatoprotective, anti-inflammatory, antiangiogenic, antitumor, antidepressant, antineurodegenerative, HIV-1 inhibitory and antioxidant effects. The aim of this study was to investigate the ability of RA to prevent chemically induced chromosome breakage or loss and primary DNA damage using the micronucleus and comet assays with V79 cells, respectively. The chemotherapeutic agent doxorubicin (DXR; 0.5 microg ml(-1)) was used as the DNA-damaging agent. The cultures were treated with different concentrations of RA (0.28, 0.56 and 1.12 mm) alone or in combination with DXR. The results showed that RA exerted no genotoxic effect, but significantly reduced the frequency of micronuclei and the extent of DNA damage induced by DXR at the three concentrations tested. The antioxidant activity of RA might be involved in the reduction of DXR-induced DNA damage observed in the present study.

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Section: Discussionmentioning

confidence: 95%

Protective effect of rosmarinic acid on V79 cells evaluated by the micronucleus and comet assays

Furtado

Araújo

Resende

et al. 2009

J of Applied Toxicology

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“…These reactive nitrogen species can have toxic effect on mitochondria and cause damage to macromolecules, such as DNA, proteins, and lipids. A recent study has shown that Ros A can also act as an effective protector against peroxynitrite-mediated damage and as a potent inhibitor of superoxide and NO synthesis; inhibition of ROS and reactive nitrogen species formation is achieved partly by the ability of Ros A to inhibit serine phosphorylation of Iκ-Bα, an inhibitor of the transcription factor NF-κB (Qiao et al, 2005). Therefore, precise regulation of superoxide and NO production appears to be critical for the survival of islet grafts in our model.…”

Section: Discussionmentioning

confidence: 99%

Prolonged survival of islet allografts in mice treated with rosmarinic acid and anti-CD154 antibody

et al. 2008

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Pancreatic islet transplantation can correct the abnormal glucose metabolism of Type 1 diabetes. Although immunosuppressants greatly reduce the acute rejection rate in transplant patients, the long-term side effects can be debilitating. Therefore, researchers are seeking to develop new immunosuppressive regimens that induce maximal levels of immunosuppression with minor side effects. Rosmarinic acid (Ros A) is a secondary metabolite of certain herbs and has multiple biological activities, including anti-inflammatory effects. Here, we have investigated whether treatment of mice with a combination of Ros A and anti-CD154 monoclonal antibody (MR1) improves islet allograft survival in a murine model. After transplantation, the mice were treated with either Ros A, MR1, or both (the "double" treatment). Allograft survival was prolonged in the double-treated animals compared to animals that received only Ros A or MR1. As is the case with the single-treated animals at 15 days after transplantation, the double-treated recipients did not display a significant decrease in the expression of cytokines or the population of activated T cells. Infiltrating CD3 + T cells were reduced in the MR1-or double therapy relative to control or RosA group. However, at the same time point, double-treated graft showed fewer apoptotic cells and increased expression of insulin and glucagons, compared to the single-treatment groups. Furthermore, long-term (＞ 150 days) allografts that were received with double therapy exhibited larger islet clusters and contained more insulin-and glucagon-positive cells, relative to the MR1-treated grafts. In conclusion, treatment with both Ros A and MR1 has a synergistic effect in murine islet allotransplantation.

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“…Cell growth inhibition of mimosine was determined by MTT reduction assay as previously described [36]. C6 glioma cells plated in 96-well plates were treated with mimosine at different concentrations (50-500 lM).…”

Section: Measurement Of Cell Viabilitymentioning

confidence: 99%

Mimosine-Induced Apoptosis in C6 Glioma Cells Requires the Release of Mitochondria-Derived Reactive Oxygen Species and p38, JNK Activation

et al. 2011

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Growth-inhibitory effects of mimosine, a plant amino acid, on rat C6 glioma cells were analyzed. Mimosine markedly inhibited proliferation and induced apoptosis of C6 glioma cells in a dose- and time-dependent manner. Mimosine-mediated apoptosis was accompanied by promoting reactive oxygen species (ROS) generation in mitochondria, and by decreased mitochondrial membrane potential (Δψ), and release of cytochrome c from mitochondria, followed by caspase 3 activation. Furthermore, mimosine increased the phosphorylation level of c-Jun-N-terminal protein kinase and p38, which was the downstream effect of ROS accumulation. Mimosine was confirmed to show profound effects on apoptosis of C6 glioma cells by ROS-regulated mitochondria pathway, and these results bear on the hypothesized potential for mimosine as promising agents in the treatment of malignant gliomas.

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Rosmarinic acid inhibits the formation of reactive oxygen and nitrogen species in RAW264.7 macrophages

Cited by 119 publications

References 17 publications

Protective effect of rosmarinic acid on V79 cells evaluated by the micronucleus and comet assays

Protective effect of rosmarinic acid on V79 cells evaluated by the micronucleus and comet assays

Prolonged survival of islet allografts in mice treated with rosmarinic acid and anti-CD154 antibody

Mimosine-Induced Apoptosis in C6 Glioma Cells Requires the Release of Mitochondria-Derived Reactive Oxygen Species and p38, JNK Activation

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