Rosmarinic acid production in hairy root cultures of Agastache rugosa Kuntze

Lee, Sook Young; Xu, Hui; Kim, Yong Kyung; Park, Sang Un

doi:10.1007/s11274-007-9560-y

Cited by 55 publications

(38 citation statements)

References 18 publications

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“…& C.A.Mey.) Kuntze hairy roots increased 14 days after culturing (Lee et al, 2008). Increased production of RA in hairy roots of Plectranthus barbatus Andrews (Coleus forskohlii) and Salvia miltiorrhiza Bunge has been reported (Li et al, 2005;Yan et al, 2006).…”

Section: Introductionmentioning

confidence: 96%

Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

Nourozi

Hosseini

Hassani

2016

AAS

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Agrobacterium rhizogenes is known as a natural tool of genetic engineering in many plant species. For the first time, hairy root induction in Agastache foeniculum using A. rhizogenes, rosmarinic acid content and the effect of different culture media and inoculation methods on hairy root growth rate were investigated. Hairy root culture of A. foeniculum was established by inoculation of the 1-month-old leaf explant with A4 strain of A. rhizogenes and the effectiveness of lightdark conditions and two inoculation methods (immersion and injection) were tested. Furthermore, in immersion method, the effects of inoculation time (3, 5 and 7 min) on root induction were investigated. In the second part of the study, the hairy root culture of A. foeniculum was studied using different basal culture media (MS, 1/2 MS and B5). Rosmarinic acid content in hairy roots and non-transformed roots was analyzed using high-performance liquid chromatography (HPLC). There was no significant difference between various inoculation methods in the ability of hairy roots induction. Observations showed that percentage of hairy root induction was higher when the explants were immersed for 5 min in bacterial suspension. Light conditions displayed the highest hairy root induction rates compared with dark condition. Various culture media are different in terms of types and amounts of nutrients and have influence on growth rate. The maximum growth rate (1.61 g fr wt/50 ml) of hairy roots were obtained in 1/2 MS medium. Rosmarinic acid content in transformed roots (213.42 µg/g dry wt) was significantly higher than non-transformed roots (52.28 µg/ g dry wt). Key words: Agastache foeniculum, Agrobacteriumrhizogenes, culture medium, hairy roots, immersion, injection, rosmarinic acid IZVLEČEK VPLIV RAZLIČNIH DEJAVNIKOV NA INDUKCIJO LASASTIH KORENIN PRI JANEŽNEM OŽEPU (Agastache foeniculum (Pursh) Kuntze)Bakterija Agrobacterium rhizogenes je znana kot naravno orodje genskega inženiringa pri mnogih rastlinskih vrstah. V tej raziskavi so prvič preučevali indukcijo lasastih korenin pri janežnem ožepu (Agastache foeniculum (Pursh.) Kuntze) z uporabo bakterije A. rhizogenes, vsebnost rožmarinske kisline in vpliv različnih gojišč ter inokulacijskih metod na rast lasastih korenin. Kulturo lasastih korenin janežnega ožepa so vzgojili iz izsečkov enomesečnih listov inokuliranih z bakterijo A. rhizogenes sev A4 z uporabo dveh inokulacijskih metod -potapljanje in vbrizgavanje, v razmerah na svetlem in v temi. Pri metodi potapljanja izsečkov v bakterijsko suspenzijo so preučevali tudi vpliv časa (3, 5 in 7 min) na indukcijo korenin. V drugem delu raziskave so preučevali vpliv različnih osnovnih gojišč (MS, 1/2 MS in B5) na kulturo lasastih korenin. Vsebnost rožmarinske kisline v transformiranih lasastih koreninah in netransformiranih koreninah so analizirali s HPLC metodo. Značilna razlika v indukciji lasastih korenin v odvisnosti od metod inokulacije ni bila ugotovljena. Opazovanja so pokazala, da je bil odstotek indukcije lasastih korenin večji, če so izs...

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Section: Introductionmentioning

confidence: 96%

Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

Nourozi

Hosseini

Hassani

2016

AAS

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“…Production of rosmarinic acid in hairy root cultures was observed by Lee et al (2008) in Agastache rugsa and by Lee at al. (2010) in Nepeta cataria L. Many studies have pointed out that hairy root growth and metabolite production depend on 'rol' gene expression (Bulgakov 2008).…”

Section: Rosmarinic Acid Production In Hairy Rootsmentioning

confidence: 68%

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

2014

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Hairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes (A4, A7 and 9435), were used for induction of hairy roots in four various explants (hypocotyl, cotyledon, onemonth-old leaf and five-month-old leaf) of Anise hyssop. The highest frequency of transformation was achieved using A4 strain in one-month-old leaves (51.1%). The transgenic states of hairy root lines were confirmed by PCR (Polymerase chain reaction) method. High performance liquid chromatography analysis revealed that the production of rosmarinic acid (RA) in transformed roots of A. foeniculum was almost 4-fold higher than that of the non-transformed roots. In a separate experiment, hairy roots obtained from one-month-old leaves inoculated with A4 strain, were grown in liquid medium and the effects of different concentrations of salicylic acid (0.0, 0.01, 0.1 and 1 mM) and chitosan (0, 50, 100 and 150 mg L −1 ) (as elicitor) and sucrose (20, 30, 40 and 50 g L −1 ) on the growth of hairy roots were evaluated. The results showed that, 30 g L −1 sucrose and 100 mg L −1 chitosan increased the biomass of hairy root cultures and application of salicylic acid reduced the growth of hairy roots compared with control roots.

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“…MTCC 2364 was less efficient (29.3%) than R1000, but much more efficient than MTCC 532 (18.6%) [31]. R1000 strain was widely used for hairy root induction in many plants such as Torenia fournieri [32], Gentiana macrophylla [33], Centella asiatica [34] and Agastache rugosa [35]. This intrinsic capacity of Ri plasmid must be higher in R1000 when compared to other strains as inferred by other reports also [36].…”

Section: Agrobacterium Rhizogenes Mediated Hairy Root Culturementioning

confidence: 92%

Efficient Agrobacterium Rhizogenes Mediated Hairy Root Culture System for Withanolide Production from Withania Somnifera (L.) Dunal

Cs¹,

Aslam²,

K³

et al. 2017

JABB

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Withania somnifera (L.) Dunal is an important medicinal plant with many pharmaceutical and therapeutic uses ranging from immunomodulation to anticancer property. Field cultivation of W. somnifera is time consuming and it not able to meet current global demands. For commercial production of withanolides, field grown plant materials have generally been used but the quality and uniformity in the levels of active constituents are highly affected by genotype and environmental conditions. In vitro culture methods are the best alternative and have possible ways to enhance commercial production of withanolides. In this review, we summarize our recent findings on characterization and screening of wild accessions of W. somnifera collected from Tamil Nadu state. Further, an improved Agrobacterium rhizogenes mediated hairy root culture system was developed and this system providing an efficient tool attaining better transformation efficiency and useful for commercial in vitro production of withanolides.

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Rosmarinic acid production in hairy root cultures of Agastache rugosa Kuntze

Cited by 55 publications

References 18 publications

Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

Influences of various factors on hairy root induction in Agastache foeniculum (Pursh) Kuntze

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

Efficient Agrobacterium Rhizogenes Mediated Hairy Root Culture System for Withanolide Production from Withania Somnifera (L.) Dunal

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