Rostro-caudal patterning of receptor-expressing olfactory neurones in the rat nasal cavity

Strotmann, Jörg; Wanner, Ina B.; Helfrich, T.; Beck, A.; Breer, Heinz

doi:10.1007/bf00305773

Cited by 152 publications

(76 citation statements)

References 22 publications

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“…1 A). For the class II genes expressed in the OCAM-positive (non-zone 1) region, hybridization signals were confined to specific areas, as it has been reported previously (Ressler et al, 1993;Vassar et al, 1993;Strotmann et al, 1994) (Fig. 1 A).…”

Section: In Situ Hybridization Of Oe Sectionssupporting

confidence: 82%

“…Each olfactory sensory neuron (OSN) expresses only one functional OR gene in a monoallelic manner (Chess et al, 1994;Malnic et al, 1999;Ishii et al, 2001;Serizawa et al, 2003), and expression of each OR gene is confined to a restricted area in the olfactory epithelium (OE) (Ressler et al, 1993;Vassar et al, 1993;Strotmann et al, 1994;Sullivan et al, 1996). Furthermore, OSNs expressing a given OR gene project their axons to a specific set of glomeruli in the olfactory bulb (OB) (Ressler et al, 1994;Vassar et al, 1994;Mombaerts et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Continuous and Overlapping Expression Domains of Odorant Receptor Genes in the Olfactory Epithelium Determine the Dorsal/Ventral Positioning of Glomeruli in the Olfactory Bulb

Miyamichi¹,

Serizawa²,

Kimura³

et al. 2005

J. Neurosci.

284

327

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In mammals, olfactory signals received by odorant receptors (ORs) in the olfactory epithelium (OE) are converted to a topographical map of activated glomeruli in the olfactory bulb (OB). It has been reported that the OE can be divided into four topographically distinct zones and that olfactory sensory neurons (OSNs) expressing a particular OR gene are randomly distributed within one zone. Here, we analyzed 80 different class II OR genes for their expression patterns in the OE by in situ hybridization. It was found that the expression area in the OE does not always fit into one of the four conventional zones. Expression areas are specific to each OR gene and are arranged in an overlapping and continuous manner in the OE. We also analyzed a spatial relationship between the OE and the OB for OSN projection. Our transgenic as well as DiI retrograde staining experiments demonstrated that the dorsal/ventral arrangement of glomeruli in the OB is correlated with the expression areas of corresponding ORs along the dorsomedial/ventrolateral axis in the OE. The present study indicates that the OR gene choice may be more restricted by the OSN location in the OE than what has been thought.

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Section: In Situ Hybridization Of Oe Sectionssupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Continuous and Overlapping Expression Domains of Odorant Receptor Genes in the Olfactory Epithelium Determine the Dorsal/Ventral Positioning of Glomeruli in the Olfactory Bulb

Miyamichi¹,

Serizawa²,

Kimura³

et al. 2005

J. Neurosci.

284

327

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“…OSNs expressing a specific OR are scattered within four spatially distinct zones of the OE (Strotmann et al, 1992(Strotmann et al, , 1994Ressler et al, 1993;Vassar et al, 1993Vassar et al, , 1994Mombaerts et al, 1996;Sullivan et al, 1996;Tsuboi et al, 1999). Although initial studies suggested that these zones only partially overlap at their boundaries, recent results suggest that OR expression domains form a continuous gradient in the OE (Miyamichi et al, 2005).…”

Section: Expression Patterns Of Robo-2 and Slits In The Olfactory Systemmentioning

confidence: 99%

“…Olfactory sensory neurons (OSNs) that are located in the olfactory epithelium (OE) form highly stereotyped connections with second-order neurons within the olfactory bulb (OB). OSNs distributed within four spatially distinct zones of the OE project axons to four specific regions of the olfactory bulb (Strotmann et al, 1992(Strotmann et al, , 1994Ressler et al, 1993Ressler et al, , 1994Vassar et al, 1993Vassar et al, , 1994Mombaerts et al, 1996;Sullivan et al, 1996;Tsuboi et al, 1999). Although these zones are defined based on the segregated patterns of expression of odorant receptors in OSNs, there exists a certain degree of overlap in the expression patterns of olfactory receptors (ORs) within some of these zones (Norlin and Berghard, 2001;Iwema et al, 2004;Miyamichi et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Requirement for Slit-1 and Robo-2 in Zonal Segregation of Olfactory Sensory Neuron Axons in the Main Olfactory Bulb

Cho¹,

Lépine²,

Andrews³

et al. 2007

J. Neurosci.

112

125

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The formation of precise stereotypic connections in sensory systems is critical for the ability to detect and process signals from the environment. In the olfactory system, olfactory sensory neurons (OSNs) project axons to spatially defined glomeruli within the olfactory bulb (OB). A spatial relationship exists between the location of OSNs within the olfactory epithelium (OE) and their glomerular targets along the dorsoventral axis in the OB. The molecular mechanisms underlying the zonal segregation of OSN axons along the dorsoventral axis of the OB are poorly understood. Using robo-2 Ϫ/Ϫ (roundabout) and slit-1 Ϫ/Ϫ mice, we examined the role of the Slit family of axon guidance cues in the targeting of OSN axons during development. We show that a subset of OSN axons that normally project to the dorsal region of the OB mistarget and form glomeruli in the ventral region in robo-2 Ϫ/Ϫ and slit-1 Ϫ/Ϫ mice. In addition, we show that the Slit receptor, Robo-2, is expressed in OSNs in a high dorsomedial to low ventrolateral gradient across the OE and that Slit-1 and Slit-3 are expressed in the ventral region of the OB. These results indicate that the dorsal-to-ventral segregation of OSN axons are not solely defined by the location of OSNs within the OE but also relies on axon guidance cues.

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“…In the chicken, catfish, and zebra fish, neurons expressing a particular OR gene appear to be randomly distributed throughout the entire olfactory epithelium (5,7,8,12). In contrast, studies performed with rodents have revealed that the OR genes are symmetrically distributed within the olfactory epithelium (13) throughout four broad zones, which consist of a series of elongated bands that extend along the anterior-posterior axis of the nasal cavity (2,14,15). Zonal segregation does not seem to be essential for coding a correct odor message since chicken and catfish, which efficiently detect odorants, show random distributions of OR genes within their olfactory epithelium.…”

mentioning

confidence: 99%

Olfaction: Transient expression of a putative odorant receptor in the avian notochord

Nef

Nef²

1997

Proc. Natl. Acad. Sci. U.S.A.

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In vertebrates, odors are thought to be detected by a multigene family encoding several hundreds of seventransmembrane-domain G-protein-coupled receptors found in fish, rat, mouse, dog, and human. Recently, the putative odorant receptor (OR) gene family in the chicken has been characterized. Twelve members have been isolated and subdivided into six subfamilies. Herein, we have further characterized the chicken olfactory receptor subfamily 7 (COR7) composed of two highly related genes (named COR7a and COR7b) which are 98.5% identical. By in situ hybridization experiments, both COR7a and COR7b transcripts were detected in the olfactory epithelium from embryonic day 6 (E6) to the new born stage. Within the olfactory epithelium, the spatial distribution of COR7a and COR7b labeled cells was random. We also observed that every individual positive cell did not coexpress the COR7a and COR7b genes. Interestingly, the COR7b gene was found to be transiently expressed in the notochord from E2 to E6, whereas COR7a or any of the other known members of the COR gene family were not detected in this mesodermal tissue. These data suggest that, in addition to its potential role as an OR in the olfactory system, COR7b may also have a function in the notochord that is essential for the dorsoventral organization of the neural tube and of the somitic mesoderm. We also discuss the possible role(s) of a putative OR present in both the notochord and the sensory olfactory epithelium.

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Rostro-caudal patterning of receptor-expressing olfactory neurones in the rat nasal cavity

Cited by 152 publications

References 22 publications

Continuous and Overlapping Expression Domains of Odorant Receptor Genes in the Olfactory Epithelium Determine the Dorsal/Ventral Positioning of Glomeruli in the Olfactory Bulb

Continuous and Overlapping Expression Domains of Odorant Receptor Genes in the Olfactory Epithelium Determine the Dorsal/Ventral Positioning of Glomeruli in the Olfactory Bulb

Requirement for Slit-1 and Robo-2 in Zonal Segregation of Olfactory Sensory Neuron Axons in the Main Olfactory Bulb

Olfaction: Transient expression of a putative odorant receptor in the avian notochord

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