Rot fluoreszierende Proteine: spezielle Anwendungen in der Bildgebung und Perspektiven

Shcherbakova, Daria M.; Subach, Fedor V.; Verkhusha, Vladislav V.

doi:10.1002/ange.201200408

Cited by 4 publications

(1 citation statement)

References 128 publications

(146 reference statements)

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“…The spectral diversity and wide range of photophysical behaviors of FPs have enabled their application in many advanced imaging techniques (Dedecker et al, 2013). Reversibly switchable red fluorescent proteins (rsRFPs) are of particular interest due to their great promise for applications such as dual-color super-resolution microscopy when combined with green rsFPs (Shcherbakova et al, 2012;Miyawaki et al, 2012;Lavoie-Cardinal et al, 2014). Unfortunately, the suboptimal photophysical behavior of most currently used RFPs has restricted their utility in super-resolution microscopy techniques (Siegel et al, 2013;Marx, 2018).…”

Section: Introductionmentioning

confidence: 99%

An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1.4

Bui¹,

Dedecker²,

Meervelt³

2023

Acta Cryst Sect F

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rsCherryRev1.4 has been reported as one of the reversibly photoswitchable variants of mCherry, and is an improved version with a faster off-switching speed and lower switching fatigue at high light intensities than its precursor rsCherryRev. However, rsCherryRev1.4 still has some limitations such as a tendency to dimerize as well as complex photophysical properties. Here, the crystal structure of rsCherryRev1.4 was determined at a resolution of 2 Å and it was discovered that it forms a dimer that shows disulfide bonding between the protomers. Mutagenesis, gel electrophoresis and size-exclusion chromatography strongly implicate Cys24 in this process. Replacing Cys24 in rsCherryRev1.4 resulted in a much lower tendency towards dimerization, while introducing Cys24 into mCherry correspondingly increased its dimerization. In principle, this finding opens the possibility of developing redox sensors based on controlled dimerization via disulfide cross-linking in fluorescent proteins, even though the actual application of engineering such sensors still requires additional research.

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Section: Introductionmentioning

confidence: 99%

An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1.4

Bui¹,

Dedecker²,

Meervelt³

2023

Acta Cryst Sect F

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Highly Fluorescent Green Fluorescent Protein Chromophore Analogues Made by Decorating the Imidazolone Ring

Gutiérrez

Martínez-López

Moron

et al. 2015

Chemistry A European J

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The synthesis and photophysical behavior of an unexplored family of green fluorescent protein (GFP)-like chromophore analogues is reported. The compound (Z)-4-(4-hydroxybenzylidene)-1-propyl-2-(propylamino)-1H-imidazol-5(4 H)-one (p-HBDNI, 2 a) exhibits significantly enhanced fluorescence properties relative to the parent compound (Z)-5-(4-hydroxybenzylidene)-2,3-dimethyl-3,5-dihydro-4H-imidazol-4-one (p-HBDI, 1). p-HBDNI was considered as a model system and the photophysical properties of other novel 2-amino-3,5-dihydro-4H-imidazol-4-one derivatives were evaluated. Time-dependent DFT calculations were carried out to rationalize the results. The analogue AIDNI (2 c), in which the 4-hydroxybenzyl group of p-HBDNI was replaced by an azaindole group, showed improved photophysical properties and potential for cell staining. The uptake and intracellular distribution of 2 c in living cells was investigated by confocal microscopy imaging.

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Fluorescent PEGulated Oligourethane Nanoparticles for Long‐Term Cellular Tracing

Mondal

Sarkar

Ghosh

2016

Chemistry A European J

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We have introduced a new ABA-type amphiphilic block copolymer consisting of functional oligourethane hydrophobic blocks and two polyethylene glycol (PEG) hydrophilic blocks. The polymer was synthesized in a single step by step-growth polymerization between two monomers, namely tetraphenylethylene (TPE)-diol and hexamehylene di-isocyanate in the presence of a monofunctional impurity PEG-2000. The polymer exhibits facile self-assembly in water by synergistic effects of H-bonding and π-π interaction among the oligourethane core, leading to the formation of robust nanoparticles with remarkable aggregation-induced emission (AIE). These nanoparticles show very low critical aggregation concentration, stability over a large pH window, and excellent biocompatibility as revealed by an MTT assay. Cellular imaging with cancer cells showed facile cellular uptake and, more importantly, retention of AIE in cellular milieu for long times, which was successfully utilized for long-term cancer cell tracking.

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Rot fluoreszierende Proteine: spezielle Anwendungen in der Bildgebung und Perspektiven

Cited by 4 publications

References 128 publications

An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1.4

An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1.4

Highly Fluorescent Green Fluorescent Protein Chromophore Analogues Made by Decorating the Imidazolone Ring

Fluorescent PEGulated Oligourethane Nanoparticles for Long‐Term Cellular Tracing

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