Rotary Culture Enhances Pre-osteoblast Aggregation and Mineralization

Facer, S. R.; Zaharias, Rebecca; Andracki, Mark E.; Lafoon, John; Hunter, Stephen K.; Schneider, Galen B.

doi:10.1177/154405910508400611

Cited by 47 publications

(63 citation statements)

References 19 publications

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“…In the present study, the expression levels of ALP, COL I and RUNX2 were upregulated in cells cultured on 3D and 3D+BG compared with those on the control surface. These observations are consistent with other studies, using primary human osteoblasts in static 3D culture conditions, which demonstrated an upregulation of COL I, ALP (15,16) and RUNX2 (2,9) in osteoblastic cells cultured in 3D environments, as compared to those cultured in 2D. On the contrary, there are also data reporting a downregulation of COL I and ALP in human osteoblast 3D cultures, compared to monolayer, after 7 and 14 days without osteogenic induction (17).…”

Section: Discussionsupporting

confidence: 92%

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Bioactive Glass Particles in Two-Dimensional and Three-Dimensional Osteogenic Cell Cultures

et al. 2017

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This study aimed to investigate the influence of a three-dimensional cell culture model and bioactive glass (BG) particles on the expression of osteoblastic phenotypes in rat calvaria osteogenic cells culture. Cells were seeded on two-dimensional (2D) and threedimensional (3D) collagen with BG particles for up to 14 days. Cell viability and alkaline phosphatase (ALP) activity was performed. Cell morphology and immunolabeling of noncollagenous bone matrix proteins were assessed by epifluorescence and confocal microscopy. The expressions of osteogenic markers were analyzed using RT-PCR. Mineralized bone-like nodule formation was visualized by microscopy and calcium content was assessed quantitatively by alizarin red assay. Experimental cultures produced a growing cell viability rate up to 14 days. Although ALP activity at 7 days was higher on BG cultures, cells on 3D and 3D+BG had an activity decrease of ALP at 14 days. Three-dimensional conditions favored the immunolabeling for OPN and BSP and the expression of ALP and COL I mRNAs. BG particles influenced positively the OC and OPN mRNAs expression and calcified nodule formation in vitro. The results indicated that the 3D cultures and BG particles contribute to the expression of osteoblastic phenotype and to differentiated and mineralized matrix formation.

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Section: Discussionsupporting

confidence: 92%

“…However, there are well-established differences between cells grown in 2D culture and a three-dimensional (3D) environment (2). Researchers have been trying to make appropriate scaffolds for cell implantation to simulate the 3D environment in vivo.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Glass Particles in Two-Dimensional and Three-Dimensional Osteogenic Cell Cultures

et al. 2017

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“…In a different study, Facer et al cultured aggregates of a human preosteoblast cell line for 28 days, and found that dynamic culture (15 rpm) under osteogenic conditions resulted in increased cell aggregation and calcium and phosphate deposition [41].…”

Section: Rotating Wall Vessel Bioreactorsmentioning

confidence: 99%

Bioreactor Systems for Human Bone Tissue Engineering

Sladkova

Peppo

2014

Processes

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Critical size skeletal defects resulting from trauma and pathological disorders still remain a major clinical problem worldwide. Bone engineering aims at generating unlimited amounts of viable tissue substitutes by interfacing osteocompetent cells of different origin and developmental stage with compliant biomaterial scaffolds, and culture the cell/scaffold constructs under proper culture conditions in bioreactor systems. Bioreactors help supporting efficient nutrition of cultured cells and allow the controlled provision of biochemical and biophysical stimuli required for functional regeneration and production of clinically relevant bone grafts. In this review, the authors report the advances in the development of bone tissue substitutes using human cells and bioreactor systems. Principal types of bioreactors are reviewed, including rotating wall vessels, spinner flasks, direct and indirect flow perfusion bioreactors, as well as compression systems. Specifically, the review deals with: (i) key elements of bioreactor design; (ii) range of values of stress imparted to cells and physiological relevance; (iii) maximal volume of engineered bone substitutes cultured in different bioreactors; and (iv) experimental outcomes and perspectives for future clinical translation.

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“…[4][5][6][7] Several studies have analyzed the effect of simulated microgravity on osteoblast differentiation using established osteoblast cell lines. 5,6,10 These studies demonstrated increased cellular aggregation and enhancement of osteoblast differentiation. The conclusions drawn from these studies were that reduced gravity may activate compensatory effects on the osteoblasts as a result of the negative loading and thus lead to enhanced osteoblast differentiation.…”

mentioning

confidence: 91%

“…The conclusions drawn from these studies were that reduced gravity may activate compensatory effects on the osteoblasts as a result of the negative loading and thus lead to enhanced osteoblast differentiation. [8][9][10] It has been reported that HEPM preosteoblast cells cultured in simulated microgravity conditions as compared with standard tissue culture plastic conditions had an increased rate of mineralization. 10 In normal osteoblast cell biology, cell membrane receptors called integrins serve to adhere cells to their surrounding extracellular matrix, and when doing so, activate internal signaling cascades leading to cell growth and differentiation.…”

mentioning

confidence: 99%