Rotator cuff repair using cell sheets derived from human rotator cuff in a rat model

Abstract

To achieve biological regeneration of tendon-bone junctions, cell sheets of human rotator-cuff derived cells were used in a rat rotator cuff injury model. Human rotator-cuff derived cells were isolated, and cell sheets were made using temperature-responsive culture plates. Infraspinatus tendons in immunodeficient rats were resected bilaterally at the enthesis. In right shoulders, infraspinatus tendons were repaired by the transosseous method and covered with the cell sheet (sheet group), whereas the left infraspinatus tendons were repaired in the same way without the cell sheet (control group). Histological examinations (safranin-O and fast green staining, isolectin B4, type II collagen, and human-specific CD31) and mRNA expression (vascular endothelial growth factor; VEGF, type II collagen; Col2, and tenomodulin; TeM) were analyzed 4 weeks after surgery. Biomechanical tests were performed at 8 weeks. In the sheet group, proteoglycan at the enthesis with more type II collagen and isolectin B4 positive cells were seen compared with in the control group. Human specific CD31-positive cells were detected only in the sheet group. VEGF and Col2 gene expressions were higher and TeM gene expression was lower in the sheet group than in the control group. In mechanical testing, the sheet group showed a significantly higher ultimate failure load than the control group at 8 weeks. Our results indicated that the rotator-cuff derived cell sheet could promote cartilage regeneration and angiogenesis at the enthesis, with superior mechanical strength compared with the control. Treatment for rotator cuff injury using cell sheets could be a promising strategy for enthesis of tendon tissue engineering. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:289-296, 2017.

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

“…The supraspinatus tendon was fixed between sandpaper using cyanoacrylate glue and held vertically in a custom clamp. Specimens were preconditioned from 0 to 5% strain at a rate of 1% strain/sec for five cycles, followed by a displacement ramp to failure at a rate of 1% strain/sec …”

“…Specimens were preconditioned from 0 to 5% strain at a rate of 1% strain/sec for five cycles, followed by a displacement ramp to failure at a rate of 1% strain/sec. [21][22][23] Micro-CT Analysis Eight weeks after repair surgery (Surgery 2), eight rats per group were sacrificed and the bilateral humeral bones with SSp muscles were harvested. Specimens were fixed in 10% formalin for 48 h at room temperature.…”

Icariin Promotes Tendon-Bone Healing during Repair of Rotator Cuff Tears: A Biomechanical and Histological Study

“…A growing number of studies have shown that factors that promote osteogenesis and inhibit bone resorption, such as osteoinductive growth factors [31,32,33], also positively affect tendon-bone healing [34]. Treatments that enhance angiogenesis can also positively accelerate tendon repair [34,35,36], improve osteogenesis [17,18,37,38] and chondrogenesis [39], and theoretically promote tendon-bone healing [34,40,41,42].…”

“…Treatments that enhance angiogenesis can also positively accelerate tendon repair [34,35,36], improve osteogenesis [17,18,37,38] and chondrogenesis [39], and theoretically promote tendon-bone healing [34,40,41,42]. However, the effects of ICA on tendon-bone healing remain unclear.…”

In Vitro Comparison of 2D-Cell Culture and 3D-Cell Sheets of Scleraxis-Programmed Bone Marrow Derived Mesenchymal Stem Cells to Primary Tendon Stem/Progenitor Cells for Tendon Repair

“…Hence, in this study, we directly compared primary hTSPCs to the immortalized single cell-derived cell line hMSC-Scx by analyzing their expression profiles (tendon-, mesenchymal lineage-related and collagen cross-linking genes), migration patterns, and ability to self-assemble tendon-like three dimensional (3D) cell sheets [3]. Cell sheet formation is based on a natural establishment of cell–cell and cell–matrix connections during cell culture with subsequent folding into a 3D tube-like shape [15,16]. Cell sheet technology resembles a process very similar to native tissue morphogenesis during embryo development; it is scaffold-free, as it not only eliminates the need of natural and synthetic carriers, but also offers a model to study in vitro tenogenic differentiation.…”