Rotavirus Genotypes P[4]G9, P[6]G9, and P[8]G9 in Hospitalized Children with Acute Gastroenteritis in Rio de Janeiro, Brazil

Abstract

Fifty-three rotavirus-positive fecal specimens from children with diarrhea admitted to a Rio de Janeiro, Brazil, children's hospital between January 1997 and December 1998 were characterized for P and G types by using reverse transcription-PCR. Genotype P[4]G2 accounted for 21% of isolates, while uncommon genotypes P[8]G9, P[6]G9, and P[4]G9 accounted for 13% of the isolates

Detection of caliciviruses associated with acute infantile gastroenteritis in Salvador, an urban center in Northeast Brazil

“…Our data show the presence of RV-A G1P [8] and G9P [8], confirming that these two genotypes are those more frequently associated with RV-A illnesses worldwide (30)(31)(32)(33). In the city of Salvador, Most worldwide studies have shown that genotype HAstV-1 is the most prevalent, followed by HAstV genotype-2, -3, -4, and -5 (35)(36)(37)(38).…”

Molecular epidemiology of human P[8],G9 rotaviruses in Hungary between 1998 and 2001

“…Beginning in the mid-1990s, there was a remarkable increase in the detection of G9 strains as a cause of gastroenteritis and over the last 7 years or so, G9 rotaviruses have emerged as one of the most epidemiologically important strains worldwide in both developed and developing countries (Ramachandran et al, 1996;Griffin et al, 2000;Iturriza-Gó mara et al, 2000;Palombo et al, 2000;Widdowson et al, 2000;Araú jo et al, 2001;Bányai et al, 2004).…”

“…Some of these may only have local significance (e.g. G5 in parts of Brazil and G8 in Malawi; Bok et al, 2001b;Cunliffe et al, 2001b), but at least one serotype, G9, was demonstrated to have dispersed worldwide (Ramachandran et al, 1996;Unicomb et al, 1999;Griffin et al, 2000;Iturriza-Gó mara et al, 2000;Palombo et al, 2000;Araú jo et al, 2001;Bok et al, 2001a;Cunliffe et al, 2001a;Bányai et al, 2004).…”

“…The G9 rotaviruses identified more recently exhibited a great variety of genomic constellations, formed predominantly by reassortment of VP4 and VP7 genes into both 'long' and 'short' E-type strains. For example, P[4],G9 'short' E-type strains were identified in Thailand; P[4],G9 'long' E-type strains were found in Brazil; P[6],G9 'short' Etype strains were detected in the USA, India and Bangladesh; P[6],G9 'long' E-type strains were detected in India, Bangladesh and Kenya;and P[8],G9 'long' E-type strains circulated in the USA, Bangladesh, Libya, Cuba, Kenya, India and Japan (Unicomb et al, 1999;Griffin et al, 2000;Oka et al, 2000;Ramachandran et al, 2000;Araú jo et al, 2001;Cunliffe et al, 2001a;Jain et al, 2001;Zhou et al, 2001).…”

G and P rotavirus genotypes in stool samples from children in Teresina, State of Piauí

“…The scheme described above was intended to detect VP7 genotypes G1, G2, G3, G4, G5, G6, G8, G9, G10 and G11. The first round of P genotyping was performed by amplification of the VP8* portion of the VP4 gene, and the second round of amplification was performed with a pool of specific primers for the following P genotypes: P [1], P [4], P [5], P [6], P [7], P [8], P [9], P [10] and P [11].…”