Rotavirus Persistence in Cell Cultures: Selection of Resistant Cells in the Presence of Foetal Calf Serum

Chiarini, A; Arista, Serenella; Giammanco, Anna; Sinatra, A.

doi:10.1099/0022-1317-64-5-1101

Cited by 13 publications

(6 citation statements)

References 28 publications

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“…In monkey kidney cells, the replication cycle is fairly rapid, with maximum yields of virus being found after 10 to 12 h at 37°C when cells are infected with virus at high multiplicities (10 to 20 PFU per cell [63,216,266]). Similar cycles are found for other virus strains, but the permissiveness of different types of cultured cells to rotavirus infection (and the cytopathogenicity of different virus strains) can vary (15,57,98,132,169). These different cell lines can be expected to be studied further as efforts to identify the cell receptor and the mechanisms controlling virus-cell interactions expand.…”

Section: Gene Functions In Different Stages Of Virus Replicationmentioning

confidence: 73%

Rotavirus gene structure and function.

Estes¹,

Cohen²

1989

Microbiological Reviews

545

427

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information relevant to the biology of these viruses. These topics are covered extensively in the reviews noted above and in others (16, 96, 101, 314, 337; M. K. Estes, in B. N. Fields, ed., Virology, 2nd ed., in press). This review also highlights the usefulness of studying the rotaviruses as models to obtain basic information on protein processing, ribonucleic acid (RNA) replication, and viral morphogenesis in eucaryotic cells. Progress toward understanding what influences the genetic and antigenic variability of the rotaviruses and the outcome of rotavirus infections of the gastrointestinal tract is emphasized. This review was undertaken with the goal of identifying areas in which new research may be useful to further understand and help combat these viruses (and other members of the family Reoviridae) in the laboratory and in nature.

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Section: Gene Functions In Different Stages Of Virus Replicationmentioning

confidence: 73%

Rotavirus gene structure and function.

Estes¹,

Cohen²

1989

Microbiological Reviews

545

427

View full text Add to dashboard Cite

show abstract

“…Apresentam um amplo espectro de hospedeiro e abrangem diversas espécies de vertebrados, incluindo o homem, principalmente durante os dois primeiros anos de vida. Esses vírus fazem parte da família Reoviridae, gênero Rotavírus; possuem genoma de RNA de duplo filamento, segmentado em 11 fragmentos de tamanhos diferentes, envolvidos por três camadas protéicas concêntricas, formando um capsídio de simetria icosaédrica 4,10,11,14 .…”

Section: Introductionunclassified

“…Além disso, o tratamento convencional da água por floculação parece ser menos eficaz para a inativação de rotavírus que de outros vírus entéricos, o que contribui sobremaneira para a sua disseminação nas comunidades 3,4,9,12 . Em virtude dessas características, os rotavírus estão relacionados entre os principais agentes causadores de diarréia aguda em todo mundo, sendo responsáveis por grande parte das internações hospitalares de crianças na faixa etária de 1 a 24 meses.…”

Section: Introductionunclassified

Rotavirus detection in feces of children with acute diarrhea

Sm²,

Jc³

et al. 2000

J Pediatr (Rio J)

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ResumoObjetivo: Determinar a prevalência da infecção por rotavírus em um grupo de crianças da cidade de Natal-RN, Brasil, que apresentavam diarréia aguda e verificar a existência ou não de distribuição sazonal da infecção por esses patógenos em nosso meio.Métodos: Foram analisadas amostras fecais de 1.903 crianças de ambos os sexos, com idades variando de 1 mês a 10 anos, residentes na cidade de Natal-RN, que apresentavam episódio de diarréia aguda, no período de janeiro de 1996 a dezembro de 1998. O método utilizado foi a pesquisa de partículas virais diretamente nas fezes, através de uma reação de aglutinação passiva, empregando-se partículas de látex cobertas com anticorpos monoclonais grupo-específico anti-rotavírus.Resultados: Na população estudada, 151 crianças (7,9%) apresentaram reação positiva para a presença de partículas de rotavírus nas fezes. Considerando-se, no entanto, apenas as crianças mais suscetíveis à infecção por esse grupo de vírus, que compreende a faixa etária de 1 a 24 meses de vida, constatou-se que, de um total de 1.065 crianças examinadas, 136 (12,8%) apresentaram reação positiva para rotavírus, sendo que 96,3% tinham entre 6 e 24 meses de vida. A distribuição pelos meses do ano, dos casos de diarréia aguda de crianças que apresentaram rotavírus nas fezes revelou que a incidência da infecção por esses patógenos foi maior nos meses de julho, agosto e setembro, nos três anos estudados.Conclusões: Os resultados indicam que os rotavírus têm participação importante na etiologia da diarréia aguda em nosso meio, e que a maioria dos casos da infecção, por esses patógenos, ocorre durante os dois primeiros anos de vida, atingindo principalmente as crianças na faixa etária de 6 a 24 meses e com uma maior prevalência nos meses de julho, agosto e setembro. J. pediatr. (Rio J.). 2000; 76(4):300-304: prevalência rotavírus, diarréia, gastroenterite. AbstractObjective: To determine the prevalence of rotavirus in the etiology of acute diarrhea in children from Natal city, RN, Brazil and investigate the existence or not of a seasonal distribution of this pathogen in our environment.Methods: Fecal samples from 1,903 children (boys and girls) with ages ranging from 1 month to 10 years, living in Natal-RN, who presented acute diarrhea episodes in a period from January 1996 to December 1998, were analyzed. We searched viral particles directly in the feces by a passive agglutination reaction using anti-rotavirus specific-group monoclonal antibodies coated latex particles.Results: 151 children (7.9%) of the studied population presented a positive reaction, revealing the presence of rotavirus particles in feces. Considering, however, only the children (ages from 1 to 24 months) who are more susceptible to rotavirus infection, we verified that from 1,065 examined children, 136 of them (12.8%) presented positive reaction for rotavirus, and the great majority of all children with positive reaction (96.3%) had ages ranging from 6 to 24 months. Analysis of the distribution of the cases of rotavirus infection in the...

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“…Coevolution of cells and virus has been described in the course of persistence of several animal viruses in cell culture (Takemoto & Habel, 1959;Ahmed et al, 1981;Chiarini et al, 1983;Delli Bovi et al, 1984;Ron & Tal, 1985, 1986Cummings & Rinaldo, 1989;Dermody et al, 1993;Calvez et al, 1995;Chen & Baric, 1996;Mrukowicz et al, 1998;Zhong et al, 2006). In other persistent infections in cell culture, no cell evolution was observed, and persistence was associated with defective interfering particles or with small-plaque and ts viral mutants (Igarashi et al, 1977;Holland et al, 1980Holland et al, , 1982Youngner & Preble, 1980).…”

Section: Discussionmentioning

confidence: 99%

Persistence of foot-and-mouth disease virus in cell culture revisited: implications for contingency in evolution

Herrera

Grande-Pérez

Perales

et al. 2008

Journal of General Virology

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If we could rewind the tape of evolution and play it again, would it turn out to be similar to or different from what we know? Obviously, this key question can only be addressed by fragmentary experimental approaches. Twenty-two years ago, we described the establishment of BHK-21 cells persistently infected with foot-and-mouth disease virus (FMDV), a system that displayed as its major biological feature a coevolution of the cells and the resident virus in the course of persistence. Now we report the establishment of two persistently infected cell lines in parallel, starting with the same clones of FMDV and BHK-21 cells used 22 years ago. We have asked whether the evolution of the two newly established cell lines and of the earlier cell line would be similar or different. The main conclusions of the study are: (i) the basic behaviour characterized by virus-cell coevolution is similar in the three carrier cell lines, despite differences in some genetic alterations of FMDV; (ii) a strikingly parallel behaviour has been observed with the two newly established cell lines passaged in parallel, unveiling a deterministic virus behaviour during persistence; and (iii) selective RT-PCR amplifications have detected imbalances in the proportion of positive-versus negative-strand viral RNA, mediated by both viral and cellular factors. The results confirm coevolution of cells and virus as a major and reproducible feature of FMDV persistence in cell culture, and suggest that rapidly evolving viruses may constitute adequate test systems to probe the influence of historical contingency on evolutionary events.

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Rotavirus Persistence in Cell Cultures: Selection of Resistant Cells in the Presence of Foetal Calf Serum

Cited by 13 publications

References 28 publications

Rotavirus gene structure and function.

Rotavirus gene structure and function.

Rotavirus detection in feces of children with acute diarrhea

Persistence of foot-and-mouth disease virus in cell culture revisited: implications for contingency in evolution

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