Rotenone impairs hippocampal neuronal activity in a rat model of Parkinson’s disease

Darbinyan, Lilit; Hambardzumyan, L. E.; Simonyan, K. V.; Chavushyan, V. A.; Manukyan, Larisa; Sarkisian, V.H.

doi:10.1016/j.pathophys.2017.01.001

Cited by 10 publications

(2 citation statements)

References 37 publications

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“…However, such depolarization could be reversed by its activation of K ATP channels and the increased excitability tends to be depressed by its suppression of I Na . Taken together, the cellular electrophysiological effects of Rot on membrane ion currents inherently in mHippoE-14 cells might significantly contribute to its neurotoxic actions in vivo [38]. …”

Section: Discussionmentioning

confidence: 99%

Multiple Actions of Rotenone, an Inhibitor of Mitochondrial Respiratory Chain, on Ionic Currents and Miniature End-Plate Potential in Mouse Hippocampal (mHippoE-14) Neurons

Huang

Lin

Hung

et al. 2018

Cell Physiol Biochem

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Background/Aims: Rotenone (Rot) is known to suppress the activity of complex I in the mitochondrial chain reaction; however, whether this compound has effects on ion currents in neurons remains largely unexplored. Methods: With the aid of patch-clamp technology and simulation modeling, the effects of Rot on membrane ion currents present in mHippoE-14 cells were investigated. Results: Addition of Rot produced an inhibitory action on the peak amplitude of I_Na with an IC₅₀ value of 39.3 µM; however, neither activation nor inactivation kinetics of I_Na was changed during cell exposure to this compound. Addition of Rot produced little or no modifications in the steady-state inactivation curve of I_Na. Rot increased the amplitude of Ca²⁺-activated Cl^- current in response to membrane depolarization with an EC₅₀ value of 35.4 µM; further addition of niflumic acid reversed Rot-mediated stimulation of this current. Moreover, when these cells were exposed to 10 µM Rot, a specific population of ATP-sensitive K⁺ channels with a single-channel conductance of 18.1 pS was measured, despite its inability to alter single-channel conductance. Under current clamp condition, the frequency of miniature end-plate potentials in mHippoE-14 cells was significantly raised in the presence of Rot (10 µM) with no changes in their amplitude and time course of rise and decay. In simulated model of hippocampal neurons incorporated with chemical autaptic connection, increased autaptic strength to mimic the action of Rot was noted to change the bursting pattern with emergence of subthreshold potentials. Conclusions: The Rot effects presented herein might exert a significant action on functional activities of hippocampal neurons occurring in vivo.

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Section: Discussionmentioning

confidence: 99%

Multiple Actions of Rotenone, an Inhibitor of Mitochondrial Respiratory Chain, on Ionic Currents and Miniature End-Plate Potential in Mouse Hippocampal (mHippoE-14) Neurons

Huang

Lin

Hung

et al. 2018

Cell Physiol Biochem

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“… 43 At the same time, other authors reviewed the effects of exposure to the insecticide deltamethrin for a period of 60 days on the behavior and brain plasticity of laboratory mice. 44 The authors noted a deficit in learning and memory and a significant reduction in neurogenesis in the hippocampus (37%) of animals treated with pesticides, suggesting that the changes promoted by pesticides in hippocampal plasticity directly influence cerebral information processing. 45 Simultaneously, several studies have revealed that paraquat, an ammonium compound used as herbicide, reduces neurogenesis and directly affects the survival and fate of new neurons generated in the hippocampus.…”

Section: How Pesticides Can Affect Neurogenesismentioning

confidence: 99%

Neurogenesis and pesticides: news of no new neurons

Scorza,

Almeida,

Fiorini

et al. 2024

Arq Neuropsiquiatr

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New hippocampal neurons are continuously generated in the adult human brain. Several studies have demonstrated that the proliferation of hippocampal cells is strongly influenced by a variety of stimuli, including pesticides exposure. These effects are particularly important because neurogenesis dysregulation could be associated with the decline of neuronal and cognitive functions and the possible development of neuropsychiatric disorders.

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Protective effects of curcumin against rotenone-induced rat model of Parkinson’s disease: in vivo electrophysiological and behavioral study

et al. 2017

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Curcumin is a naturally occurring phenolic yellow chemical isolated from the rhizomes of the plant Curcuma longa (turmeric), and is a major component of the spice turmeric. Curcumin has protective effects against rotenone-induced neural damage in Parkinson's disease (PD). The present study aims at providing new evidence for the validity of the rotenone rat model of PD by examining whether neuronal activity in the hippocampus is altered. Male albino rats were treated with rotenone injections (2.5 mg/ml intraperitoneally) for 21 days. We examined the effects of curcumin (200 mg/kg) on behavior and electrophysiology in a rat model of PD induced by rotenone. Motor activity was assessed by cylinder test. The electrical activity of neurons was measured in hippocampus. Rotenone causes significant reduction of neuronal activity. The results show that curcumin can improve the motor impairments and electrophysiological parameters and may be beneficial in the treatment of PD.

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Rotenone impairs hippocampal neuronal activity in a rat model of Parkinson’s disease

Cited by 10 publications

References 37 publications

Multiple Actions of Rotenone, an Inhibitor of Mitochondrial Respiratory Chain, on Ionic Currents and Miniature End-Plate Potential in Mouse Hippocampal (mHippoE-14) Neurons

Multiple Actions of Rotenone, an Inhibitor of Mitochondrial Respiratory Chain, on Ionic Currents and Miniature End-Plate Potential in Mouse Hippocampal (mHippoE-14) Neurons

Neurogenesis and pesticides: news of no new neurons

Protective effects of curcumin against rotenone-induced rat model of Parkinson’s disease: in vivo electrophysiological and behavioral study

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