Roundup® Herbicide Decreases Quality Parameters of Spermatozoa of Silversides Odontesthes Humensis

Acute exposition to Roundup Transorb® induces systemic oxidative stress and alterations in the expression of newly sequenced genes in silverside fish (Odontesthes humensis)

“…A c o m m e r c i a l f o r m u l a t i o n o f g l y p h o s a t e [N-(phosphonomethyl)glycine], commercially known as Roundup Transorb®, and containing 588 g L −1 potassium salt of glyphosate as the active ingredient (480 g L −1 or 48% acid equivalent of glyphosate), was used. The real concentration of glyphosate in the water contaminated with RDT (expressed as GAE) was measured in triplicate from the water samples collected from each tank as previously described (Zebral et al 2018;Silveira et al 2019b). For this analysis, Shimadzu 2010 Plus equipped with a flame photometric detector and DB-17 fused silica capillary column (30 m × 0.25 mm I.D., 0.25 μm film thickness) (J & W, USA) was used.…”

“…A concentration of 0.1-165 μg L −1 of glyphosate was detected in water bodies in European countries, such as France, Germany, Spain, and Switzerland, where the cultivation of genetically modified seeds is prohibited (Van Bruggen et al 2018). Some studies considered the glyphosate concentration of up to 10 mg L −1 as environmentally realistic; thus, relatively high concentrations of glyphosate might be present in these environments immediately after Roundup® application (Langiano and Martinez 2008;Topal et al 2015;Silveira et al 2019b). The presence of herbicides in aquatic ecosystems is associated with negative effects on various organisms, such as aquatic plants (Zhong et al 2018), micro-organisms (Wu et al 2016), and fish (Bridi et al 2017;Silveira et al 2019b).…”

“…Some studies considered the glyphosate concentration of up to 10 mg L −1 as environmentally realistic; thus, relatively high concentrations of glyphosate might be present in these environments immediately after Roundup® application (Langiano and Martinez 2008;Topal et al 2015;Silveira et al 2019b). The presence of herbicides in aquatic ecosystems is associated with negative effects on various organisms, such as aquatic plants (Zhong et al 2018), micro-organisms (Wu et al 2016), and fish (Bridi et al 2017;Silveira et al 2019b). Commercial glyphosate formulations can induce changes in bioenergetic and detoxification pathways, including the antioxidant defense system, leading to the accumulation of reactive oxygen species (ROS).…”

“…These water bodies are surrounded by agricultural-productive areas, which mainly cultivate soy and rice monocultures with the use of RDT de Brito Rodrigues et al 2017. Furthermore, O. humensis is considered a bioindicator in environmental studies because of its demands for good water quality and survival only in a narrow range of water parameters (Zebral et al 2017;Silveira et al 2018a, b;Silveira et al 2019b).…”