Routine Method for Concentration and Partial Purification of a Murine Leukaemia Virus (Rauscher)

Chenaille, P; Lévy, J. P.; Tavitian, Armand; Boiron, M

doi:10.1038/213107a0

Cited by 26 publications

(9 citation statements)

References 3 publications

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“…A standard Moloney murine sarcoma virus was obtained from homogenates of 10-12-day-old tumors developed in newborn NMRI mice inoculated with 0.95 ml of diluted 1:10 stock virus, kindly supplied by Dr. E. DeClerq of the Rega Institute, Belgium, The tumors were homogenized in a glass homogenizer according to the method described by Chanaille et al [14]. The supernatant was centrifuged at 30,000 • g for 60 min and stored in -30~ until use.…”

Section: Preparation Of Virusmentioning

confidence: 99%

Virally induced periosteal osteogensis in mice

Włodarski¹,

Kobus²,

Łuczak³

et al. 1981

Calcif Tissue Int

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Inoculation of Moloney murine sarcoma virus (M-MSV) into shank muscles of adult NMRI mice resulted in localized sarcoma and periosteal membrane proliferation with subsequent periosteal bone formation. Newly formed bone arose from outer surface of shank bones. Two weeks after M-MSV inoculation the width of bone cortex increased nearly 4 times. The spaces between newly formed bone ossicles were filled with bone marrow. In the later stages these bone marrow cavities were merged with medullar cavities. No extraskeletal bone formation was observed. The regression of M-MSV-induced sarcomas coincides with cessation of the proliferation of bone therefore allowing the maturation and rebuilding of bone. Newly formed bone was not resorbed during a 6-month observation period.

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Section: Preparation Of Virusmentioning

confidence: 99%

Virally induced periosteal osteogensis in mice

Włodarski¹,

Kobus²,

Łuczak³

et al. 1981

Calcif Tissue Int

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“…This virus was recovered from a thymic lymphoma induced in a WKA/Mk rat neonatally injected with Gross virus by a modification of Chenaille's method (Chenaille et al, 1967). Standard preparations of Gross virus (Lots 0627-72) were used.…”

Section: Virusmentioning

confidence: 99%

Spontaneous breakdown of immunological tolerance to gross‐virus‐induced cell‐surface antigens in the rat

Kuzumaki

Takeichi

Kodama

et al. 1973

Intl Journal of Cancer

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“…Die intraperitoneale Inokulation solcher Ultrafiltrate in neugeborene BALB/c-Mäuse ergab kein Tumorwachstum [33], Aus HIPA-Tumoraszites der 19. Tumorgeneration konnte dagegen mit der Methode nach C henaii.ee et al [7] ein Ultrazentrifugat hergestellt werden, das onkogene Viren Typus C enthielt und dessen intraperitoneale Inokulation in 2-21 Tage alten BALB/c-Mäusen zu mesenterialen Plas mozytomen führte [32]. Trotz dieses ersten Erfolges gelang eine weitere zellfreie Passage damals nicht, da zu wenig Aszites für die Aufbereitung des Ultrazentrifugates gewonnen werden konnte.…”

Section: Virusätiologie Der H/pa -Neoplasieunclassified

“…Nach 2 Tagen bekamen die so behandelten Mäuse zellfreie Extrakte aus HIPA-Tumoraszites, die mit der Ultrazentrifuga tionsmethode nach C henaille et al [7] Onkogene Virus-C-Partikel wurden in verschiedenen Tumorgenerationen nach gewiesen und nach der sogenannten «Doppelinokulationsmethode» extrahiert. Die i.p.-Inokulation so gewonnener zellfreier Extrakte verursacht in isogenen und allogenen Mäusen mesenteriale Plasmozytome.…”

Section: Virusätiologie Der H/pa -Neoplasieunclassified

Das experimentelle HIPA-Plasmozytom der Maus

Pedio¹

1974

Pathobiology

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HIPA tumor is the denomination of an experimental plasmacytoma of mice. It can be transmitted by inoculation of cell-free extracts in syngenic or allogenic mice or by tumor cell transplant in syngenic mice. HIPA tumor was originally induced in the mesentery of BALB/c mice by intraperitoneal injection of spleen homogenate, obtained from a syngenic mouse, pretreated with incomplete Freund’s adjuvant. The original HIPA tumor was histologically an undifferentiated sarcoma. It underwent differentiation to plasmacytoma in the course of several transplant generations. This differentiation was characterized by loss of collagen fibers and appearance of annulate lamellae and dilatation of ergastoplasmic cysternae. HIPA tumor presented the following characteristics: (a) secretion of abnormal proteins in the course of the 3rd to 28th transplant generation; (b) appearance of a thrombocytopenía in the host after intraperitoneal inoculation of tumor cells; (c) formation of osteolytical and osteoplastic bone lesions after inoculation of tumor cells; (d) absence of splenic metastases regardless of the route of tumor inoculation. Oncogenic virus-like type C and A particles were found in the spleen of mineral oil-treated BALB/c mice and in HIPA tumor tissues of numerous transplant generations. After repeated attempts to isolate and enrich HIPA oncogenic virus by conventional methods, a double inoculation method was devised. It consisted of an initial intraperitoneal transplantation of HIPA tumor cells followed by intraperitoneal injection of HIPA cell-free tumor extracts. This procedure was repeated in several subsequent generations. Cell-free extracts of tumors which developed after double inoculation contained numerous virus-like type C particles. Such extracts induced plasmacytomas in syngenic and allogenic mice.

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Routine Method for Concentration and Partial Purification of a Murine Leukaemia Virus (Rauscher)

Cited by 26 publications

References 3 publications

Virally induced periosteal osteogensis in mice

Virally induced periosteal osteogensis in mice

Spontaneous breakdown of immunological tolerance to gross‐virus‐induced cell‐surface antigens in the rat

Das experimentelle HIPA-Plasmozytom der Maus

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