Routine Methods in Toxicology and Therapeutic Drug Monitoring by High Performance Liquid Chromatography. II. A Rapid Microscale Method for Determination of Chloramphenicol in Blood and Cerebrospinal Fluid

Microbore Liquid Chromatography for Pediatric and Neonatal Therapeutic Drug Monitoring and Toxicology: Clinical Analysis of Chloramphenicol

“…Chloramphenicol may b e quantified by microbiological assay (3, radioenzymatic assays, gas chromatographic assays($), and readily by L C with high specificity (5)(6)(7)(8). R e c e n t novel LC procedures included Sood's study (9) using small sample size of 25 UL for a two s t e p extraction, followed by L C analysis, and El-Yazigi's study(l0) of direct-sampleanalysis of 10 UL by reversed-phase(RP) LC. However, t h e l a t e r study did not establish column stability.…”

“…R e c e n t studies also showed t h e interest of reduced sample size. Sood utilized 25 UL of serum for a multi-step extraction(9), while El-Yazigi demonstrated direct-sample-analysis with 10 UL sampIes(l0). However, column stability was not established for this later study.Protein precipitation was achieved by vortex-mixing with t h e methanolic solution of t h e internal standard.…”