Routine Methods in Toxicology and Therapeutic Drug Monitoring by High-Performance Liquid Chromatography. IV. A Rapid Microscale Method for Determination of Propranolol and 4-Hydroxypropranolol in Plasma

Sp, Sood; Vi, Green; Rp, Mason

doi:10.1097/00007691-198802000-00018

Cited by 10 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, reversephase liquid chromatography using an isocratic elution system has been preferred over those previously described. Consequently, in the present study the binary system using reverse-phase chromatography was found to be the best alternative among the most complex systems reported in the literature [14][15][16]19 . Despite the good selectivity obtained with complex systems, an evident limitation of the reported methods is the high flow rate (1.5-2.0 mL/min) required to elute the drug 11-13, 20, 21 .…”

Section: Validation Of the Analytical Methodsmentioning

confidence: 74%

“…Different methods have been developed to determine plasma propranolol concentrations, including fluorimetry 7,8,9 , gas chromatography 10 , and high-performance liquid chromatography with fluorescence detection (HPLC-F) [11][12][13][14][15][16] . More recently, an analytical method for the quantification of propranolol in plasma of postoperative adult patients was proposed by Pereira et al (2000).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Propranolol Plasma Monitoring in Children Submitted to Surgery of Tetralogy of Fallot by a Micromethod Using High Performance Liquid Chromatography

Sanches

Galas

Silva

et al. 2007

Clinics

View full text Add to dashboard Cite

Sanches C, Galas FRBG, Silva AGO de M, Carmona MJC, Auler Jr. JO, Santos SRCJ. Propranolol plasma monitoring in children submitted to surgery of tetralogy of Fallot by a micromethod using high performance liquid chromatography. Clinics. 2007;62(3):215-24. OBJECTIVE:To evaluate the analytical micromethod using liquid chromatography for the quantification of propranolol in children submitted to surgery of tetralogy of Fallot (TLF). Methods: Only 0.2 mL of plasma is required for the assay. Peaks eluted at 8.4 (Propranolol) and 17.5 min (verapamil, internal standard) from a C18 column, with a mobile phase 0.1 M acetate buffer, pH 5.0, and acetonitrile (60:40, v/v) at flow rate 0.7 mL/min, detected at 290 nm (excitation) and 358 nm (emission). Surgery was started 776 min of drug administration (8.7mg, mean); seven blood samples were collected from six patients (4M/2F; 2.1yrs;11.5kg; 0.80m; 18.9kg/m 2 ). RESULTS: Confidence limits of the method showed high selectivity and recovery, sensitivity of 0.02ng/mL, good linearity (0.05-1000ng/mL), precision of 8.6% and accuracy of 3.1%. The mean duration of surgery was 283.2min, with the patients remaining under cardiopulmonary bypass (CPB) for 114min. A declining curve of propranolol plasma concentration was obtained after the last dose in the night that preceded the day of surgery. Plasma concentration also was normalized with hematocrit due to the hemodilution caused by the CPB procedure. On the other hand a decrease on drug plasma concentration was obtained between periods, the beginning of surgery to the postoperative day 2 (7.09 ng/mL and 0.05 ng/mL, p<0.05 respectively) and from the end of CPB to the postoperative day 2 (2.79ng/mL e 0.05ng/mL, p<0.05). CONCLUSION: Propranolol monitoring of plasma concentrations of children (TLF) normalized after the last preoperative dose revealed a decline from the beginning of surgery to the second postoperative day, suggesting that, once redistribution was restored, propranolol washout was complete.

show abstract

Section: Validation Of the Analytical Methodsmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Propranolol Plasma Monitoring in Children Submitted to Surgery of Tetralogy of Fallot by a Micromethod Using High Performance Liquid Chromatography

Sanches

Galas

Silva

et al. 2007

Clinics

View full text Add to dashboard Cite

show abstract

“…All of these methods reported involve time-consuming sample preparation, which complicates routine analysis. Most require labor-intensive organic extraction, often followed by back-extraction into aqueous acid (Sood et al, 1988;Nation et al, 1978;Lo et al, 1982;Kwong and Shen, 1987). Methods involving solid-phase extraction (Harrison et al, 1985) and acetonitrile precipitation (Lo and Riegelman, 1980) do not significantly reduce sample preparation time.…”

Section: Resultsmentioning

confidence: 99%

“…This method may sometimes result in high reference values that are variable from day to day in the same patient (Chidsey et al, 1975). Several bianalytical methods for propranolol along with their advantages and disadvantages have been reported (Sood et al, 1988). The gasliquid chromatography (GLC) procedure of Di Salle et al (1973) was based on a lengthy and laborious procedure of solvent extraction of propranolol from plasma after appropriate pH adjustment followed by derivatization.…”

Section: Introductionmentioning

confidence: 99%

Determination of propranolol concentration in small volume of rat plasma by HPLC with fluorometric detection

Kim

Hong

Park

et al. 2001

Biomedical Chromatography

View full text Add to dashboard Cite

A simple, rapid and sensitive fluorescence high performance liquid chromatographic method was developed to determine propranolol concentration in the small volume of rat plasma without the solvent extraction step using pronethanol as the internal standard. The analysis was accomplished using a 5 microm CAPCELL PAK analytical cyano column at room temperature and a mobile phase consisted of 1% aqueous acetic acid containing 0.2% triethylamine and acetonitrile (65:35, v/v; pH 3.8). The flow-rate was kept at 0.5 mL/min and column effluent was monitored with a fluorescence detector at an excitation wavelength of 230 nm and an emission wavelength of 340 nm. Retention times for pronethalol and propranolol were 8.5 min and 10.5 min, respectively. Linear regressions for the standard curves were linear in the range 2-800 ng/mL, giving correlation coefficients above 0.998. The detection limit was 1.34 ng/mL. No analytical interference was observed from endogenous components in rat plasma. This simple and sensitive assay method was feasibly applied to the pharmacokinetic study of propranolol after intravenous administration of 2 mg/kg of propranolol to normal and carbon tetrachloride-induced liver cirrhotic rats.

show abstract

“…Concentrations of more than 13.2 µmol/L (5 mg/L) are associated with toxicity (8). Caffeine and doxapram can be measured by high-performance liquid chromatography (HPLC) (27,28). Caffeine can also be measured by immunoassay (29).…”

Section: Neonatal Apneamentioning

confidence: 99%

Review: Therapeutic Drug Monitoring in Pediatrics

Soldin¹,

Soldin²

2002

Therapeutic Drug Monitoring

View full text Add to dashboard Cite

Therapeutic drug monitoring (TDM) can be defined as the measurement of drug concentrations in biologic fluids to assess whether they correlate with the patient's clinical condition and whether the dosage or dosage intervals need to be changed. This is done to optimize the management of patients receiving drug therapy for the alleviation or prevention of disease. Therapeutic drug monitoring is a relatively new service in the clinical pharmacology and toxicology laboratory and has evolved from being a luxury to a necessity. The principles of TDM were developed in the 1960s. Advances in research and knowledge and increasingly sophisticated laboratory methods led to an expansion of TDM (1-3).A drug must meet the following criteria to be eligible for monitoring:1. There should be a clinically interpretable correlation between the serum drug concentration and its pharmacologic effect. This usually implies a clinically significant correlation between the serum drug concentration and its concentration in the target tissue (1). There should be a better correlation between the plasma drug concentration and the pharmacologic effect than between the drug dosage and the pharmacologic effect. 2.A narrow margin should exist between serum concentrations that cause toxic effects and concentrations that produce therapeutic effects. 3.The serum concentration resulting from a given drug dose is unpredictable as a result of inter-and intraindividual differences in drug absorption, distribution, and elimination. Such poor correlation between serum concentration and drug dosage has been shown with clomipramine used in treatment of enuresis (2). 4.The pharmacologic effects of drugs are not readily measurable (e.g., suppression of seizure activity is difficult to monitor clinically when administering anticonvulsant drugs). 5.There must be a rapid and reliable method for the analysis of the drug.The criteria for monitoring drugs in children are the same as those for adults (6), but several additional factors must be considered. Neonates, infants, and children undergo major and rapid age-related physiologic and biochemical changes, especially during the first year of life, resulting in different clinical pharmacokinetic and pharmacodynamic parameters from adults (Table 1). Recent indications are that approximately 12% of all drugs prescribed in the United States are for children age 9 years and younger (4). Further, review of drug-

show abstract

Routine Methods in Toxicology and Therapeutic Drug Monitoring by High-Performance Liquid Chromatography. IV. A Rapid Microscale Method for Determination of Propranolol and 4-Hydroxypropranolol in Plasma

Cited by 10 publications

References 0 publications

Propranolol Plasma Monitoring in Children Submitted to Surgery of Tetralogy of Fallot by a Micromethod Using High Performance Liquid Chromatography

Propranolol Plasma Monitoring in Children Submitted to Surgery of Tetralogy of Fallot by a Micromethod Using High Performance Liquid Chromatography

Determination of propranolol concentration in small volume of rat plasma by HPLC with fluorometric detection

Review: Therapeutic Drug Monitoring in Pediatrics

Contact Info

Product

Resources

About