RP - HPLC Method Development and Validation for Simultaneous Estimation of Emtriacitabine, Efavirenz and Tenofovir Disproxil Fumarate in Pharmaceutical Dosage Forms

Abstract

A simple new spectrophotometric method has been developed for estimation of Tenofovir disoproxil fumarate in bulk and tablet dosage form. Tenofovir disoproxil fumarate is estimated to be 261 nm in triple distilled water. The Beer′s law is obeyed in the concentration range of 5 -90 µg/ml of the drug.The slope and intercept values are 0.0109 and 0.1075, respectively. Results of analysis of this method have been validated statically and by recovery studies. The method is applied to the marketed tablet formulation. A result of the analysis of tablet formulation, given as a percentage of label claim ± standard deviation is 98.15 ± 0.76. The precision and accuracy has been examined by performing recovery studies and found to be 100.06 ± 1.24. The developed method is simple, sensitive, and reproducible, and can be used for the routine analysis of Tenofovir disoproxil fumarate in bulk and tablet dosage form.