RP-HPLC Method for Simultaneous Determination of Captopril and Diuretics: Application in Pharmaceutical Dosage Forms and Human Serum

Sultana, Najma; Arayne, M. Saeed; Sattar, Naveed

doi:10.4172/2157-7064.1000109

Cited by 23 publications

(16 citation statements)

References 17 publications

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“…Harlikar et al (2003) have reported simultaneous determination of a few ACE-inhibitors like perindopril, ramipril and trandolapril along with indapamide. Sultana et al (2010Sultana et al ( , 2011 and El-Gindy et al (2013) reported the analysis of captopril, indapamide and related compounds using HPLC. Other methods utilizing HPLC for simultaneous determination of ACE-Inhibitors were reported by Bonazzi et al (1997) and Manna et al (2001).…”

Section: Captoprilmentioning

confidence: 99%

HPLC-PDA analysis of ACE-inhibitors, hydrochlorothiazide and indapamide utilizing design of experiments

Dawud

Shakya

2019

Arabian Journal of Chemistry

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A simple, rapid, precise, accurate and sensitive high performance liquid chromatographic method has been developed for simultaneous determination of ACE inhibitors with hydrochlorothiazide and indapamide in pharmaceutical formulations. 'Design of Experiments' (DoE) using 'central composite design' (CCD) was applied to facilitate method development and optimization. Mobile phase was optimized utilizing response surface methodology using Design Expert software. Chromatographic separation was achieved on HypersilÒ-Gold C 18 (100 · 4.6 mm, 3 lm, Thermo Fisher Scientific, USA), column at 25°C. The mobile phase was 58% buffer (5 mM KH 2 PO 4 , containing triethylamine 0.25 ml/L), 25% acetonitrile and 17% methanol (pH adjusted to 2.8 ± 0.1). The analysis was performed at 215 nm. The mobile phase flow rate was 1.0 ml/min and injection volume 10 ll. The method was validated for linearity, limits of quantitation and detection, accuracy, precision, ruggedness and robustness as per the International Conference on Harmonization (ICH) guidelines. Calibration curves (for lisinopril, hydrochlorothiazide, captopril, imidapril, perindopril, indapamide and trandolapril) were linear in the concentration range of 5-35 lg/ml. The limit of detection and limit of quantitation for experimental drugs ranged from 0.03 to 0.61 and 0.08-1.84 lg/ml respectively. ª 2014 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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Section: Captoprilmentioning

confidence: 99%

HPLC-PDA analysis of ACE-inhibitors, hydrochlorothiazide and indapamide utilizing design of experiments

Dawud

Shakya

2019

Arabian Journal of Chemistry

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“…The solution from the extraction (20 mL) was injected to HPLC by chromatographic conditions chosen using C18 column (250 × 4.6 mm, 5 µm), UV-Vis detector [11]. Measurements were taken at 0, 0.5, 1, 1.5, 2, 2.5, 3, 4, 8, and 10 hrs after administration of the furosemide.…”

Section: Examination Of Furosemide Levels In the Bloodmentioning

confidence: 99%

Effect of 70% Ethanol Extract of Orthosiphonis Stamineus Benth Leaves on the Pharmacokinetic Parameters of Furosemide in Male White Rats

2017

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Objective: This study aimed to observe the effect of the 70% ethanol extract of Orthosiphonis stamineus Benth leaves on the pharmacokinetic parameters of furosemide in white male rats.Methods: 18 Sprague-Dawley male rats were divided into three groups: The normal control group was given only 1% carboxymethyl cellulose, the furosemide group was given 7.2 mg/200 g body weight (BW) suspension of furosemide, and the combination group was given 700 mg/kg BW suspension of the 70% ethanolic extract of O. stamineus Benth leaves for 4 days followed by a 7.2 mg/200 g BW suspension of furosemide. On the 4 th day of treatment, we performed orbital sinus blood sampling on the eyes of the rats and analyzed the levels of furosemide in plasma using highperformance liquid chromatography.Results: Therefore, the results showed that the administration of the 70% ethanol extract of O. stamineus Benth leaves improves the pharmacokinetic parameters of furosemide on Cp max and the area under the curve (p<0.05). Conclusion:This study concludes that the 70% ethanol extract of O. stamineus Benth leaves improves the pharmacokinetic parameters of furosemide in white male rats.

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“…Our research colleagues have reported a number of liquid chromatographic methods with UV detection for different classes of simultaneously prescribed drugs including statins [26], NSAIDs [27], diuretics [28], ACE inhibitors [29], quinolones [30], H 1 -receptor antagonists [31], H 2 -receptor antagonists [32], anti-diabetic [33] drugs and so on. Almost, all of these methods have been developed and validated isocratically at isobestic point of studied drugs.…”

Section: Introductionmentioning

confidence: 99%

Ultra Sensitive Liquid Chromatographic Method for the Simultaneous Determination of Carbamazepine with Nsaids in API, Pharmaceutical Formulation and Human Serum by Programming the Detector: Application to In Vitro Drug Interaction

Sultana¹

2012

J Chromat Separation Techniq

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Here we describe an ultra-sensitive reverse phase liquid chromatographic method for the simultaneous determination of carbamazepine with NSAIDs in API, pharmaceutical formulation and human serum with UV detection. The separation of these multiple drug components was made by a Bondapak C 18 column (10 µm, 25 cm×0.46 cm) using a moderate acidic mixture of mobile phase i.e., methanol:water 80:20 with pH adjusted to 3.0 using 85% o-phosphoric acid. The flow rate of the mobile phase was affixed at 1.0 ml min -1 for the best minimum interval of elusion of all the analytes. The detector response was monitored at isobestic point of 220 nm at ambient temperature. Calibration curves were obtained in the range of 0.4-12 µg mL -1 for carbamazepine, 0.5-16 µg mL -1 for meloxicam and 0.25-8.0 µg mL -1 for ibuprofen and mefenamic acid. Limits of detection were found to be 4.0, 3.0, 1.0, and 13.0 ng mL -1 respectively. The method was compared by programming the detector at individual wavelength of components which showed more sensitivity with linear range 0.10-3.0, 0.15-5.0, 0.10-3.0 and 0.125-4.0 µg mL -1 and detection limits 2.0, 2.0, 1.0, and 3.0 ng mL -1 respectively. ICH guidelines were followed for validation study. The method showed good precision and accuracy within acceptable range and can be successfully applied for the determination of these drugs in pharmaceutical formulations, human serum and clinical laboratories without interference of excipients or endogenous components of serum. In vitro interaction studies have also been carried out at physiological temperature in buffers of various pH (4, 7.4 and 9.0) simulating human stomach environments and % availability has been calculated. Moreover, the solid charge transfer complexes of carbamazepine with interacting NSAIDs were synthesized and characterized by IR spectroscopy and verified by computational molecular modeling.

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RP-HPLC Method for Simultaneous Determination of Captopril and Diuretics: Application in Pharmaceutical Dosage Forms and Human Serum

Cited by 23 publications

References 17 publications

HPLC-PDA analysis of ACE-inhibitors, hydrochlorothiazide and indapamide utilizing design of experiments

HPLC-PDA analysis of ACE-inhibitors, hydrochlorothiazide and indapamide utilizing design of experiments

Effect of 70% Ethanol Extract of Orthosiphonis Stamineus Benth Leaves on the Pharmacokinetic Parameters of Furosemide in Male White Rats

Ultra Sensitive Liquid Chromatographic Method for the Simultaneous Determination of Carbamazepine with Nsaids in API, Pharmaceutical Formulation and Human Serum by Programming the Detector: Application to In Vitro Drug Interaction

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