RRM Transcription Factors Interact with NLRs and Regulate Broad-Spectrum Blast Resistance in Rice

Zhai, Keran; Deng, Yiwen; Liang, Di; Tang, Jun; Liu, Jing; Yan, Bingxiao; Yin, Xin; Lin, Hui; Chen, Fudan; Yang, Dongyong; Xie, Zhen; Liu, Ji-Yun; Li, Qun; Zhang, Lin; He, Zhonghu

doi:10.1016/j.molcel.2019.03.013

Cited by 96 publications

(79 citation statements)

References 60 publications

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“…By contrast, the higher PigmS level in panicle suppresses PigmR‐activated immune responses, which favors reproductive growth and in agriculture could reduce the yield loss caused by strong immunity. This case highlights that regulation of paired antagonistic NLRs is a novel mechanism balancing plant immunity and development (Deng et al 2017; Zhai et al 2019). NLR clusters seem a common phenomenon in the plant genome, and several recent reviews have made detailed discussions on this topic (van Wersch and Li 2019).…”

Section: Recent Discoveries Of the Activation Of Intracellular Nlrsmentioning

confidence: 96%

Plant immune signaling: Advancing on two frontiers

Wang

Feng

Zhou

et al. 2020

JIPB

188

144

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Plants have evolved multiple defense strategies to cope with pathogens, among which plant immune signaling that relies on cell-surface localized and intracellular receptors takes fundamental roles. Exciting breakthroughs were made recently on the signaling mechanisms of pattern recognition receptors (PRRs) and intracellular nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domain receptors (NLRs). This review summarizes the current view of PRRs activation, emphasizing the most recent discoveries about PRRs' dynamic regulation and signaling mechanisms directly leading to downstream molecular events including mitogen-activated protein kinase (MAPK) activation and calcium (Ca 2+ ) burst. Plants also have evolved intracellular NLRs to perceive the presence of specific pathogen effectors and trigger more robust immune responses. We also discuss the current understanding of the mechanisms of NLR activation, which has been greatly advanced by recent breakthroughs including structures of the first full-length plant NLR complex, findings of NLR sensor-helper pairs and novel biochemical activity of Toll/interleukin-1 receptor (TIR) domain.

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Section: Recent Discoveries Of the Activation Of Intracellular Nlrsmentioning

confidence: 96%

Plant immune signaling: Advancing on two frontiers

Wang

Feng

Zhou

et al. 2020

JIPB

188

144

View full text Add to dashboard Cite

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“…Regarding BPA genes, 60 alternative splicing events that lead to the peptide change were identified from 24% (39 out of 161) plant members, with 23 events causing the disruption of RRM_1 domain. RRM domain containing protein has important roles in regulating plant defense (Zhai et al 2019), and domain-disrupted isoforms often exhibit remarkably different functions when compared with their corresponding normal proteins (Finet et al 2013). Therefore, the widespread occurrence of alternative splicing in plant BPAs may also increase their functional diversity.…”

Section: Discussionmentioning

confidence: 99%

“…Motif 1 corresponds to RRM_1 (RNA recognition motif) domain. It was recently reported that the domain-containing gene may play a key role in plant immunity (Zhai et al 2019) while none of other motifs can be found in the Pfam database. They distribute unevenly in different clades and angiosperm groups with motif 10 being specific to fungi.…”

Section: Bpa Genes Exhibit Early Divergence In Angiospermsmentioning

confidence: 99%

Genome-wide identification and molecular evolution analysis of BPA genes in green plants

Zhang

Wang

et al. 2020

Phytopathol Res

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Reactive oxygen species (ROS) signaling plays a central role in plant immune response. BPAs, referred to as binding partner 1 of accelerated cell death 11 (ACD11) (BPA1) and BPA1-like proteins, regulate ROS-mediated defense responses in Arabidopsis thaliana. However, their distribution and evolutionary characteristics in the plant lineage remain unexplored. In this study, we demonstrated that most BPA genes form a plant-specific family with expansion events observed. We found that BPA and ACD11 genes co-exist in all land plants, suggesting that this immune regulatory module may originate at the early stage of land plant emergence and contribute to their colonization. Angiosperm BPAs can be classified into four distinct groups (I-IV) in our analysis. Domain organization and motif composition are highly conserved within each group but divergent across different groups. In certain species, BPAs undergo complex alternative splicing, suggesting their regulatory and functional divergence. The protein-protein interaction network we constructed predicted additional acting partners of BPAs. The yeast twohybrid assay revealed 15 BPA interaction pairs forming homo-or hetero-dimers. Taken together, our results provide the first synopsis of BPA evolutionary pattern and adaptations to green plant colonization.

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“…A recent study suggested that the LRR domain of ZAR1 plays an important role in interaction with the decoy RKS1, while also helping to maintain ZAR1 in an autoinhibition state through intramolecular interactions (Wang et al , 2019a). Both the CC and TIR domains have been shown to function as signalling components in plant NLRs (Swiderski et al , 2009; Bernoux et al , 2011; Baudin et al , 2017; Wang et al , 2018; Zhai et al , 2019). Indeed, the CC or TIR domains of numerous NLR proteins can trigger cell death when transiently expressed in Nicotiana benthamiana (Bernoux et al , 2011; Bai et al , 2012; Wang et al , 2015; Cesari et al , 2016; Hamel et al , 2016; Baudin et al , 2017; Kim et al , 2018).…”

Section: Introductionmentioning

confidence: 99%

Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance

Gao

Gong

et al. 2020

Molecular Plant Pathology

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Molecular Plant Pathology. 2020;21:975-984. | 975 wileyonlinelibrary.com/journal/mpp | INTRODUC TI ONPlants prevent pathogen invasion via a two-layered immune system (Dodds and Rathjen, 2010;Dangl et al., 2013). The first layer is pattern-triggered immunity (PTI) governed by membrane-localized pattern recognition receptors (PRRs). PTI is the basal immune response of plants against infections by pathogens (Couto and Zipfel, 2016;Tang et al., 2017). Once pathogens suppress PTI and enter host cells, plants employ the second layer of the immune system to combat pathogens.In this branch of the immune system, intracellular nucleotide-binding Abstract Nucleotide-binding (NB) leucine-rich repeat (LRR) receptors (NLRs) provide resistance against several plant pathogens. We previously cloned the wheat powdery mildew resistance gene Pm21, which encodes a coiled-coil (CC) NLR that confers broad-spectrum resistance against Blumeria graminis f. sp. tritici. Here, we report comprehensive biochemical and functional analyses of Pm21 CC domain in Nicotiana benthamiana. Transient overexpression assay suggested that only the extended CC (eCC, amino acid residues 1-159) domain has cell-death-inducing activity, whereas the CC-containing truncations, including CC-NB and CC-NB-LRR, do not induce celldeath responses. Coimmunoprecipitation (Co-IP) assay showed that the eCC domain self-associates and interacts with the NB and LRR domains in planta. These results imply that the activity of the eCC domain is inhibited by the intramolecular interactions of different domains in the absence of pathogens. We found that the LRR domain plays a crucial role in D491V-mediated full-length (FL) Pm21 autoactivation. Some mutations in the CC domain leading to the loss of Pm21 resistance to powdery mildew impaired the CC activity of cell-death induction. Two mutations (R73Q and E80K) interfered with D491V-mediated Pm21 autoactivation without affecting the cell-death-inducing activity of the eCC domain. Notably, some susceptible mutants harbouring mutations in the CC domain still exhibited cell-death-inducing activity. Taken together, these results implicate the CC domain of Pm21 in cell-death signalling and disease-resistance signalling, which are potentially independent of each other. K E Y W O R D S CC domain, cell death, disease resistance, intramolecular interaction, Pm21 S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Gao A, Hu M, Gong Y, et al. Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance. Molecular Plant Pathology. 2020;21:975-984.

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RRM Transcription Factors Interact with NLRs and Regulate Broad-Spectrum Blast Resistance in Rice

Cited by 96 publications

References 60 publications

Plant immune signaling: Advancing on two frontiers

Plant immune signaling: Advancing on two frontiers

Genome-wide identification and molecular evolution analysis of BPA genes in green plants

Pm21 CC domain activity modulated by intramolecular interactions is implicated in cell death and disease resistance

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