RT‐PCR tests for sensitive detection of the major <i>Ranunculus</i>‐infecting viruses: field and <i>in vitro</i> applications

Sacco, E.; Borghi, C.; Rabaglio, M.; Lenzi, R.; Ciuffo, M.; Ruffoni, B.; Vaira, A. M.

doi:10.1111/ppa.12912

Cited by 3 publications

(1 citation statement)

References 28 publications

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“…TSWV and INSV continue to cause severe disease (131), producing necrosis of leaves, stems, and petioles with possible premature plant death. Occurrence of virus-like symptoms prompted surveys (107,109,130) along the Italian Riviera, where Ranunculus is cultivated for export. Previously reported viruses and three new potyviruses were identified: ranunculus leaf distortion virus, ranunculus mild mosaic virus, and ranunculus mosaic virus; a new macluravirus, artichoke latent virus (20), was also detected.…”

Section: Virus Diseases Of Ranunculus (Buttercup)mentioning

confidence: 99%

International Trade and Local Effects of Viral and Bacterial Diseases in Ornamental Plants

Huang

Jordan

Meekes³

et al. 2023

Annu. Rev. Phytopathol.

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Since the 1950s, there have been major changes in the scope, value, and organization of the ornamental plant industry. With fewer individual producers and a strong trend toward consolidation and globalization, increasing quantities of diverse plant genera and species are being shipped internationally. Many more ornamentals are propagated vegetatively instead of by seed, further contributing to disease spread. These factors have led to global movement of pathogens to countries where they were not formerly known. The emergence of some previously undescribed pathogens has been facilitated by high-throughput sequencing, but biological studies are often lacking, so their roles in economic diseases are not yet known. Case studies of diseases in selected ornamentals discuss the factors involved in their spread, control measures to reduce their economic impact, and show some potential effects on agronomic crops. Advances in diagnostic techniques are discussed, and parallels are drawn to the international movement of human diseases. Expected final online publication date for the Annual Review of Phytopathology, Volume 61 is September 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Section: Virus Diseases Of Ranunculus (Buttercup)mentioning

confidence: 99%

International Trade and Local Effects of Viral and Bacterial Diseases in Ornamental Plants

Huang

Jordan

Meekes³

et al. 2023

Annu. Rev. Phytopathol.

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A next-generation-sequencing approach to diagnose viral diseases of greenhouse ranunculus

et al. 2022

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First Report of Ranunculus Mild Mosaic Virus in Ranunculus asiaticus in Korea

Kwak

Byun

Choi³

et al. 2022

Plant Disease

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Ranunculus (Ranunculus asiaticus L.) is a popular ornamental plant mainly cultivated for cut flowers and flowering potted plants. In January 2021, a leaf sample of R. asiaticus that showed virus-like symptoms including mosaic, yellowing and malformation on leaves was collected from a greenhouse in Jangheung, South Korea for disease diagnosis (Fig. S1). Disease incidence was greater than 30% in the greenhouse (~1,000 m2). Transmission electron microscopy (TEM) of symptomatic leaves identified potyvirus-like filamentous virus particles of about 800 nm. To confirm the TEM results, a symptomatic leaf sample was further analyzed by reverse-transcription polymerase chain reaction (RT-PCR) using species-specific detection primers for six potyviruses that infect R. asiaticus (Sacco et al., 2018). The sample was positive only for ranunculus mild mosaic virus (RanMMV). Additional analysis of nine symptomatic R. asiaticus plants from the infected greenhouse found that all samples were positive for RanMMV. To exclude the presence of the other viruses, next generation sequencing (NGS) was carried out. Total RNA was extracted from symptomatic leaves using the RNeasy Plant Mini Kit (Qiagen, Germany) and a transcriptome library was generated using the TruSeq Stranded Total RNA LT Sample Prep kit (Illumina, San Diego, CA) acccording to the recommended protocol. NGS was performed using an Illumina NovaSeq 6000 system (Macrogen Inc., Korea). A total of 75.58 million reads were obtained, and the reads were de novo assembled to contigs using Trinity software (Grabherr et al., 2011). BLASTn and BLASTx analysis of the contigs against the NCBI viral reference database identified the assembled large contig of 9,539 nt (5,321 mapped reads, mean read coverage of 84.2 times) as RanMMV. This sequence shared 98% nt identity (99% coverage) with the RanMMV NL isolate (acc. no. LC604020) isolated from an anemone plant (A. blanda cv. Charmer) from Netherlands. To obtain the complete genome sequence, the termini sequences were determined by 5′ and 3′ rapid amplification of cDNA ends (RACE) methods as reported recently (Imamura et al., 2021). The assembled full-length genome sequence of RanMMV-JH is 9,574 nt in length, excluding the poly(A) tail, and encoding a polyprotein of 3,074aa. The sequence was deposited in GenBank under the accession no. OL742438. RanMMV is transmitted by aphids in a nonpersistent manner and has very narrow host range. RanMMV, one of causative agents of ranunculus mosaic disease, has been problematic in ranunculus production area of Japan (Hayahi et al., 2018; Kamikawa et al., 2022). Recently, some perennial weeds from the Ranunculaceae family (e.g. R. japonicus, R. silerifolius and R. tachiroei) are known to may act as a virus reservoir (Kamikawa et al., 2022). As R. asiaticus is cultivated by vegetative propagation, there is need to develop certification system for producing virus-free R. asiaticus. To our knowledge, this is the first report of RanMMV infection in R. asiaticus in Korea.

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RT‐PCR tests for sensitive detection of the major Ranunculus‐infecting viruses: field and in vitro applications

Cited by 3 publications

References 28 publications

International Trade and Local Effects of Viral and Bacterial Diseases in Ornamental Plants

International Trade and Local Effects of Viral and Bacterial Diseases in Ornamental Plants

A next-generation-sequencing approach to diagnose viral diseases of greenhouse ranunculus

First Report of Ranunculus Mild Mosaic Virus in Ranunculus asiaticus in Korea

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