RT-qPCR assay on the vitamin D receptor gene in type 2 diabetes and hypertension patients in Turkey

Vural, Hasibe Cıngıllı; Maltas, E.

doi:10.4238/2012.march.14.1

Cited by 13 publications

(14 citation statements)

References 24 publications

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“…Of these, 14 case–control studies examined the association of the ApaI rs7975232 (G > T) variant [ 3 , 18 – 30 ] (Table 1 ), 22 studies in 20 case–control papers examined the association of the BsmI rs1544410 (A > G) variant [ 18 , 22 , 23 , 27 – 39 ] (Table 2 ), 19 studies in 18 case–control studies examined the association of the Taq1rs731236 (T > C) variant[ 3 , 18 – 28 , 32 , 33 , 35 , 38 – 40 ] (Table 3 ) and 18 studies in 16 case–control studies in15 papers examined the association of FokIrs2228570 (C > T)variant [ 3 , 18 , 23 – 25 , 27 , 30 – 32 , 36 , 41 – 45 ] (Table 4 ) with Insulin resistance related diseases susceptibility.…”

Section: Resultsmentioning

confidence: 99%

VDR Gene variation and insulin resistance related diseases

Han

Gong

et al. 2017

Lipids Health Dis

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BackgroundVitamin D status may influence the risk of Insulin resistance related diseases such as Type 2 diabetes (T2DM), metabolic syndrome (MetS), and polycystic ovarian syndrome (PCOS). Several studies have assessed vitamin D receptor (VDR) gene polymorphism in relationship with these diseases; however, results remain inconsistent. Our study was conducted to elucidate whether VDR Gene polymorphisms could predict insulin resistance on a large scale.MethodsA meta-analysis using MEDLINE and EMBASE, was performed up to December 16th, 2016. Studies reporting association of vitamin D gene polymorphism with incident T2DM, MetS and PCOS outcomes were included and sub-group analysis by pigment of skin and latitude were performed.ResultsA total of 28 articles based on four gene variation, and comprising 9232 participants with 5193 Insulin resistance related diseases patients were included. No significant associations of the VDR ApaI, BsmI, FokI and TaqI variant with Insulin resistance related diseases were found. However, sub-group analysis analysis showed that PCOS in TaqI (OR = 1.47, 95% CI = 1.03–2.09, P = 0.03) for T allele and MetS for G allele (OR = 1.41, 95% CI = 1.07–1.85, P = 0.01) in BsmI was significant association with VDR gene polymorphism. Simultaneously, sub-group analysis showed VDR ApaI rs7975232(G > T)variant was associated with insulin resistance related diseases in Asians (GG/GT + TT) (OR, 1.62; 95% CI, 1.03–2.53; P = 0.04) and population who lived in middle latitude district (30–60°) (GG/GT + TT) (OR, 1.22; 95% CI, 1.04–1.43; P = 0.02), VDR BsmI rs1544410 (A > G)and VDR Taq1rs731236 (T/C) variant were associated with insulin resistance related diseases in Caucasian (dark-pigmented).ConclusionThe results suggested that the association between insulin resistance related diseases and VDR ApaI, BsmI, FokI variant was more obvious in dark-pigmented Caucasians and Asians but not in Caucasian with white skin.

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Section: Resultsmentioning

confidence: 99%

VDR Gene variation and insulin resistance related diseases

Han

Gong

et al. 2017

Lipids Health Dis

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“…A second possibility is that vitamin D may play an insignificant role in the regulation of blood pressure. Vuralet al previously reported that there was no statistically significant difference in the genotype distributions and allele frequencies of the vitamin D receptor gene TaqI polymorphism between hypertensive patients and controls in Turkey (32). The largest randomized, double-blind, placebo-controlled trial (Women's Health Initiative, n=36,282) failed to demonstrate an impact of vitamin D supplementation on blood pressure or incident hypertension over a 7-year follow-up period (33).…”

Section: Discussionmentioning

confidence: 99%

Dissociation between Low Vitamin D Level and Hypertension in Coal Mine Workers: Evidence from the Kailuan Study

et al. 2016

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Objective The aim of this study was to evaluate the vitamin D status and the relationship between the vitamin D status and hypertension in a relatively large cohort in northern China. Methods This study was a part of the Kailuan study, consisting of 3,788 coal mine workers (including 2,532 underground workers and 1,256 surface workers) who received periodic health examinations between September 13, 2012 and December 24, 2012. Information on demographic factors, personal history and medical history were collected. The height, weight, blood pressure and serum25-hydroxyvitamin D [25(OH) D] level of each patient were measured. Results The mean 25(OH)D level in this cohort was 21.73±15.82 nmol/L. The number (%) of patients with vitamin D deficiency, insufficiency, inadequacy and sufficiency were 2,509 (66.24%), 1,051 (27.75%), 201 (5.31%) and 27 (0.71%), respectively. In all the participants, after adjusting for the age, salt intake, physical activity, smoking status, alcohol drinking status, work type, work environment, body mass index, diabetes and hyperlipidemia, the odds ratios for hypertension with 25(OH)D level ! 50, 25-50 and <25 nmol/L were 1.00 (reference), 1.44 (95%CI, 0.99-2.11) and 1.39 (95%CI, 0.97-1.99), respectively. Logistic regression models to evaluate the odds ratios and 95% CIs of hypertension for each quintile of the 25(OH)D level did not determine significant associations between the vitamin D status and hypertension. No significant associations were found in the underground workers or in the surface workers. Conclusion There was a high proportion of coal mine workers with vitamin D deficiency and insufficiency in Kailuan. However, no significant association between low vitamin D levels and hypertension was found in this cohort. Further investigations are needed to determine the relationship between vitamin D levels and hypertension.

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“…VDR polymorphisms have been associated with type 1 DM (T1DM) [11] and T2DM [13–15]. BsmI (A > G, rs1544410), ApaI (A > C, rs7975232), TaqI (T > C, rs731236), and FokI (C > T, rs2228570) are human VDR single-nucleotide polymorphisms (SNPs).…”

Section: Introductionmentioning

confidence: 99%

The VDR gene FokI polymorphism is associated with gestational diabetes mellitus in Turkish women

et al. 2019

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Background The association between the vitamin D receptor ( VDR ) gene and gestational diabetes mellitus (GDM) has not been investigated in Turkish pregnant women. We aimed to investigate associations between VDR gene BsmI (rs15444410), ApaI (rs7975232), FokI (rs19735810), and TaqI (rs731236) single nucleotide polymorphisms (SNPs) and GDM. Material-methods This case-control study comprised 100 women with GDM and 135 pregnant women without GDM. The VDR polymorphism was evaluated using Sanger-based DNA sequencing. Result VDR gene ApaI , BsmI , and TaqI SNPs did not differ between women with and without GDM (each, p > 0.05). ApaI , BsmI , and TaqI were not associated with GDM risk. The VDR gene FokI CT/TT genotype was associated with an increased GDM risk (CT vs. CC, OR = 1.84, 95% CI: [1.05–3.23], p = 0.031; TT vs. CC, OR = 3.95, 95% CI: [1.56–9.96], p = 0.002; CT/TT vs. CC, OR = 2.29, 95% CI: [1.35–3.89], p = 0.002; and CT/CC vs. TT, OR = 3.02, 95% CI: [1.23–7.38], p = 0.012). The FokI-TT genotype was more associated with younger age and higher glucose, HbA1c, and HOMA-IR than the CC and CT genotype. FokI - T was positively correlated with log-HOMA-IR (r = 0.326, p = 0.004). FokI SNPs were independently associated with GDM after adjusting for BMI and age (β = 1.63, 95% CI: [1. 2-4.2], p = 0.012). There were no associations between the FokI , ApaI , BsmI and TaqI haplotypes and GDM. Conclusion VDR gene FokI SNPs were independently associated with having GDM in Turkish women. VDR gene FokI SNPs might contribute to insulin resistance of developing GDM.

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RT-qPCR assay on the vitamin D receptor gene in type 2 diabetes and hypertension patients in Turkey

Cited by 13 publications

References 24 publications

VDR Gene variation and insulin resistance related diseases

VDR Gene variation and insulin resistance related diseases

Dissociation between Low Vitamin D Level and Hypertension in Coal Mine Workers: Evidence from the Kailuan Study

The VDR gene FokI polymorphism is associated with gestational diabetes mellitus in Turkish women

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