Ru(phen)32+ doped silica nanoparticle based immunochromatographic strip for rapid quantitative detection of β-agonist residues in swine urine

Xu, Wei; Chen, Xuelan; Huang, Xiaolin; Yang, Wanchun; Liu, Chunmei; Lai, Weihua; Xu, Hengyi; Xiong, Yonghua

doi:10.1016/j.talanta.2013.04.013

Cited by 55 publications

(20 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dmitriy et al established Brucella colloidal gold antibody test strip can only detect bovine serum and the serum dilution limit of detection was only 1:250 [14]. Recently, a new labelled and more sensitive method was developed with uorescent microspheres [15,16]. So, in present study, we established and developed an immunochromatography brucellosis diagnostic method labelled with QD orescent microspheres.…”

Section: Introductionmentioning

confidence: 95%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic test strip (ICTS) based on quantum dots (QDs) for detection the antibodies of Brucella infection animals serum.Result: The test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. The detection results were achieved by using the ratio of the fluorescence signals of the test and control lines (HT/HC) with a threshold of 0.0492. The repeatability was excellent with an overall average CV of 8.78%. Under optimum conditions, the limit of detection was 1.05 ng/mL (1:512 dilution). With regard to the detection of brucellosis in 150 clinical samples, the total coincidence rate of ICTS and Rose Bengal plate test (RBPT) was 97.3%, the coincidence rate of positive samples was 98.8%, the coincidence rate of negative samples was 95.3%, and no cross reaction with the sera of other related diseases was observed.Conclusion: In our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity, high specificity and low cost.

show abstract

Section: Introductionmentioning

confidence: 95%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In previous studies, the colloidal gold test strip method was shown to have low sensitivity, and it may be di cult to detect low titer rotavirus (RV) and enteric adenovirus (AdV) [14]. An emerging detection technique uses uorescent microspheres [15,16] instead of colloidal gold particles [17]. Many researchers have recently focused on developingquantum dot uorescent microsphere immunochromatography and they have been widely used in the eld of biological detection such as microbiological detection, analytical chemistry and disease diagnosis.…”

Section: Introductionmentioning

confidence: 99%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic test strip (ICTS) based on quantum dots to detect serum antibodies of brucellosis.Result: The test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella, OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 against Brucella were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. Antibodies specific for brucellosis was detected qualitatively using the ratio of the fluorescence signals of the test and control lines (HT/HC). The threshold of assay was determined as a HT/HC value of 0.0492. The repeatability was excellent with an overall average CV of 8.78%. Under optimum conditions, the limit of detection was 1.05 ng/mL (1:512 dilution). With regard to the detection of brucellosis in 150 clinical samples, the total coincidence rate of ICTS and Rose Bengal plate test (RBPT) was 97.3%, the coincidence rate of positive samples was 98.8%, the coincidence rate of negative samples was 95.3%, and no cross reaction with the sera of other related diseaseswas observed.Conclusion: In our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity high specificity and low cost.

show abstract

“…Colloidal gold based LFIA has been applied to the qualitative detection of CLEN and salbutamol (SAL) [ 22 , 23 ]. In order to improve sensitivity of LFIA for β-agonists, labels such as Ru(phen) 3 2+ doped silica nanoparticle [ 24 ] and fluorescent nano silica [ 25 ], have been employed in LFIA fabrication. In 2015, we have developed a fluorescent beads based multi component LFIA method for simultaneous detection of CLEN, ractopamine (RAC) and SAL in feed, urine and tissue samples with higher sensitivity (as low as 0.1 ng mL −1 ) [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

et al. 2018

View full text Add to dashboard Cite

An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several β-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free β-agonists compete for the binding sites. This enables rapid screening for the 7 β-agonists in a single run that takes about 8 min. Detection limits for the seven β-agonists are <50 pg g−1 of pork. Recoveries ranged from 69.5% to 102.4%, and relative standard deviations were ±15%. The assay was applied to the analysis of both using spiked and unspiked pork for β-agonists, and the results compare well to those obtained by HPLC-MS. Graphical abstractSchematic presentation of an ultra sensitive fluorescent nanoparticle based paper based assay for rapid detection of multi β-agonists in pork tissue. Electronic supplementary materialThe online version of this article (10.1007/s00604-018-2730-9) contains supplementary material, which is available to authorized users.

show abstract

Ru(phen)32+ doped silica nanoparticle based immunochromatographic strip for rapid quantitative detection of β-agonist residues in swine urine

Cited by 55 publications

References 30 publications

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

A paper-based competitive lateral flow immunoassay for multi β-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

Contact Info

Product

Resources

About