2020
DOI: 10.1073/pnas.2007990117
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Rubisco accumulation factor 1 (Raf1) plays essential roles in mediating Rubisco assembly and carboxysome biogenesis

Abstract: Carboxysomes are membrane-free organelles for carbon assimilation in cyanobacteria. The carboxysome consists of a proteinaceous shell that structurally resembles virus capsids and internal enzymes including ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), the primary carbon-fixing enzyme in photosynthesis. The formation of carboxysomes requires hierarchical self-assembly of thousands of protein subunits, initiated from Rubisco assembly and packaging to shell encapsulation. Here we study the r… Show more

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Cited by 54 publications
(58 citation statements)
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References 64 publications
(127 reference statements)
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“…The most well-characterized BMC is the carboxysome, which acts as the key CO 2 -fixing machinery in all cyanobacteria and many chemoautotrophs 6 8 . The primary carboxylating enzymes, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), are encased in the carboxysome 9 . Based on their protein composition and phylogeny of Rubisco enzymes, carboxysomes can be categorized into two types, α-carboxysomes and β-carboxysomes 10 .…”
Section: Introductionmentioning
confidence: 99%
“…The most well-characterized BMC is the carboxysome, which acts as the key CO 2 -fixing machinery in all cyanobacteria and many chemoautotrophs 6 8 . The primary carboxylating enzymes, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), are encased in the carboxysome 9 . Based on their protein composition and phylogeny of Rubisco enzymes, carboxysomes can be categorized into two types, α-carboxysomes and β-carboxysomes 10 .…”
Section: Introductionmentioning
confidence: 99%
“…BMCs encapsulate catalytic enzymes within an icosahedral shell that serves as a physical barrier to selectively mediate flux of metabolites, while condensing and protecting cargo enzymes [15][16][17] . Carboxysomes (including α-carboxysomes and β-carboxysomes) are anabolic BMCs, functioning as the key CO 2 -fixing machinery in all cyanobacteria and some chemoautotrophs [18][19][20][21] . The primary carboxylating enzymes, ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco), and carbonic anhydrase (CA), are encased by the carboxysome shell that is constructed by diverse shell proteins through selfassembly [21][22][23][24] .…”
mentioning
confidence: 99%
“…The actual composition of the LSU 8 oligomers before binding of SSU remains confusing. LSU 8 have been crystallized with RBCX (Bracher et al, 2011), RAF1 (Huang et al, 2020;Xia et al, 2020) and BSD2 (Aigner et al, 2017). However, the last assembly intermediate is considered as a RAF1 8 -LSU 8 complex in cyanobacteria (Hauser et al, 2015;Kolesinski et al, 2017;Xia et al, 2020), but as a BSD2 8 -LSU 8 complex in plants (Aigner et al, 2017;Conlan et al, 2019).…”
Section: New Insights Into the Pathway For Rubisco Biogenesis In Vivomentioning
confidence: 99%
“…In the latter, Rubisco amount was decreased by one third, followed by a default in carboxysome formation, resulting in reduced cell growth in air (Huang et al, 2020). Interestingly, while both RAF1 and RBCX appear as non-essential in cyanobacteria and seem to be able to interact in vitro with LSU oligomers of the same order, their mode of action may be different: the resolution of LSU-containing crystals (Bracher et al, 2011;Hauser et al, 2015;Huang et al, 2020;Xia et al, 2020) indicates indeed that RBCX and RAF1 have different binding sites on LSU, and may work sequentially, as RAF1 is able to displace RBCX from LSU while the converse is not true (Kolesinski et al, 2017). Secondly, the respective knock-outs observed in cyanobacteria exhibit different phenotypes regarding Rubisco amount and carboxysome formation, casting doubts about their possible redundancy (Huang et al, 2019;Huang et al, 2020).…”
Section: Introductionmentioning
confidence: 99%