Rubisco and its regulation—major advances to improve carbon assimilation and productivity

Carmo‐Silva, Elizabete; Sharwood, Robert E.

doi:10.1093/jxb/erac475

Cited by 7 publications

(2 citation statements)

References 18 publications

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“…Overall, our results indicate that modification of CW hydroxycinnamic acid content, a unique and important feature of grass CWs, can influence mesophyll CW thickness and leaf and plant-level CO 2 -uptake and water-use without having detrimental impacts on plant growth even under LW stress. Integrating such increases in g m and A net , through changes in CW hydroxycinnamic acid content, with efforts to increase photosynthetic efficiency (Carmo-Silva and Sharwood, 2023) and decrease g sw (Leakey et al, 2019) could help achieve substantial increases in photosynthesis, biomass, and WUE under both well-watered and water limited conditions in monocot C 3 crops.…”

Section: Discussionmentioning

confidence: 99%

Altered cell wall hydroxycinnamate composition impacts leaf and canopy-level CO₂-uptake and water-use in rice

Pathare

Panahabadi

Sonawane

et al. 2023

Preprint

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Cell wall properties can play a major role in determining photosynthetic carbon-uptake and water-use through impacts on mesophyll conductance (CO2 diffusion from substomatal cavities into photosynthetic mesophyll cells) and leaf hydraulic conductance (water movement from xylem, through leaf tissue to stomata). Consequently, modification of cell wall properties is proposed as a major path for improving photosynthesis and crop water-use efficiency. We tested this using two independent transgenic rice lines that overexpress the rice OsAT10 gene (a "BAHD" CoA acyltransferase) which altered cell wall hydroxycinnamic acid content (greater para-coumaric acid and lower ferulic acid). Plants were grown under high and low water-levels and traits related to leaf anatomy, cell wall composition, gas exchange and hydraulics, plant biomass, and canopy-level water-use were measured. Alteration of hydroxycinnamic acid content led to significant decreases in mesophyll cell wall thickness (-14%), and increased mesophyll conductance (+120%) and photosynthesis (+22%). However, concomitant increases in stomatal conductance negated increased photosynthesis, resulting in no change in intrinsic water-use efficiency (ratio of photosynthesis/stomatal conductance). The leaf hydraulic conductance was also unchanged; however, the transgenics showed small, but significant increase in above-ground biomass (+12.5%), and canopy-level water-use efficiency (+8.8%; ratio of above-ground biomass/ water-used) and performed better under low water-level. Our results demonstrate that changes in cell wall composition, specifically hydroxycinnamic acid content, can increase mesophyll conductance and photosynthesis in C3 cereal crops like rice. However, attempts to improve photosynthetic water-use efficiency will need to enhance mesophyll conductance and photosynthesis whilst maintaining or decreasing stomatal conductance.

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Section: Discussionmentioning

confidence: 99%

Altered cell wall hydroxycinnamate composition impacts leaf and canopy-level CO₂-uptake and water-use in rice

Pathare

Panahabadi

Sonawane

et al. 2023

Preprint

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“…This suggests that the lesser magnitude of increase in A net in the transgenics despite greater increases in g m could be attributed to enhanced biochemical limitations in transgenics compared to WT. Alleviation of these biochemical limitations, for instance through changes in Rubisco properties and kinetics (Flexas et al, 2016;Carmo-Silva and Sharwood, 2023), could further increase A net in the OsAT10-overexpression lines.…”

Section: Cw Transgenics Show Greater G M and A Net But Not Wue Imentioning

confidence: 99%

Altered cell wall hydroxycinnamate composition impacts leaf- and canopy-level CO2 uptake and water use in rice

Pathare,

Panahabadi,

Sonawane

et al. 2023

Plant Physiology

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Cell wall properties play a major role in determining photosynthetic carbon uptake and water use through their impact on mesophyll conductance (CO2 diffusion from substomatal cavities into photosynthetic mesophyll cells) and leaf hydraulic conductance (water movement from xylem, through leaf tissue, to stomata). Consequently, modification of cell wall properties might help improve photosynthesis and crop water-use efficiency. We tested this using two independent transgenic rice (Oryza sativa) lines overexpressing the rice OsAT10 gene (encoding a “BAHD” CoA acyltransferase), which alters cell wall hydroxycinnamic acid content (more para-coumaric acid and less ferulic acid). Plants were grown under high and low water levels, and traits related to leaf anatomy, cell wall composition, gas exchange, hydraulics, plant biomass, and canopy-level water use were measured. Alteration of hydroxycinnamic acid content led to statistically significant decreases in mesophyll cell wall thickness (-14%), and increased mesophyll conductance (+120%) and photosynthesis (+22%). However, concomitant increases in stomatal conductance negated the increased photosynthesis, resulting in no change in intrinsic water-use efficiency (ratio of photosynthesis to stomatal conductance). Leaf hydraulic conductance was also unchanged; however, transgenic plants showed small but statistically significant increases in above-ground biomass (+12.5%) and canopy-level water-use efficiency (+8.8%; ratio of above-ground biomass to water used) and performed better under low water levels than wild-type plants. Our results demonstrate that changes in cell wall composition, specifically hydroxycinnamic acid content, can increase mesophyll conductance and photosynthesis in C3 cereal crops such as rice. However, attempts to improve photosynthetic water-use efficiency will need to enhance mesophyll conductance and photosynthesis whilst maintaining or decreasing stomatal conductance.

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CRISPR/Cas Systems for Enhancing Photosynthesis: Climate Resilience and Food Production

Barupal,

Kumar

2024

Gene Editing in Plants

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Rubisco and its regulation—major advances to improve carbon assimilation and productivity

Cited by 7 publications

References 18 publications

Altered cell wall hydroxycinnamate composition impacts leaf and canopy-level CO₂-uptake and water-use in rice

Altered cell wall hydroxycinnamate composition impacts leaf and canopy-level CO₂-uptake and water-use in rice

Altered cell wall hydroxycinnamate composition impacts leaf- and canopy-level CO2 uptake and water use in rice

CRISPR/Cas Systems for Enhancing Photosynthesis: Climate Resilience and Food Production

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Rubisco and its regulation—major advances to improve carbon assimilation and productivity

Cited by 7 publications

References 18 publications

Altered cell wall hydroxycinnamate composition impacts leaf and canopy-level CO2-uptake and water-use in rice

Altered cell wall hydroxycinnamate composition impacts leaf and canopy-level CO2-uptake and water-use in rice

Altered cell wall hydroxycinnamate composition impacts leaf- and canopy-level CO2 uptake and water use in rice

CRISPR/Cas Systems for Enhancing Photosynthesis: Climate Resilience and Food Production

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Product

Resources

About

Altered cell wall hydroxycinnamate composition impacts leaf and canopy-level CO₂-uptake and water-use in rice

Altered cell wall hydroxycinnamate composition impacts leaf and canopy-level CO₂-uptake and water-use in rice