Rubus idaeus L. reverses epithelial-to-mesenchymal transition and suppresses cell invasion and protease activities by targeting ERK1/2 and FAK pathways in human lung cancer cells

Hsieh, Yih‐Shou; Chu, Shu‐Chen; Hsu, Li‐Sung; Chen, Kuo-Shuen; Lai, Ming‐Tsung; Yeh, Chia-Heng; Chen, Pei‐Ni

doi:10.1016/j.fct.2013.10.021

Cited by 49 publications

(34 citation statements)

References 52 publications

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“…Moreover, we found that a higher concentration of RIE was more effective in suppressing NPC cell migration and invasion, suggesting that consumption of more Rubus idaeus as an adjuvant therapy may offer better efficacy in inhibiting metastasis. RIE has been shown to exhibit weak cytostatic effects on human cells at a concentration below 100 μg/mL (Hsieh et al, 2013;Chu et al, 2014), and thus the experimental concentrations of the extract used in the present study should be feasible in a clinical setting. Moreover, previous studied have shown that gallic acid, epigallocatechin (EGC), and catechin are the active substances in RIE (Hsieh et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…RIE has been shown to exhibit weak cytostatic effects on human cells at a concentration below 100 μg/mL (Hsieh et al, 2013;Chu et al, 2014), and thus the experimental concentrations of the extract used in the present study should be feasible in a clinical setting. Moreover, previous studied have shown that gallic acid, epigallocatechin (EGC), and catechin are the active substances in RIE (Hsieh et al, 2013). They also mentioned that RIE is able to transcriptionally regulated MMP-2 via the downregulation of ERK1/2 pathway in human lung cancer cells (Hsieh et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Raspberry or Rubus idaeus L. is a plant that belongs to the Rosaceae family. Recent studies have shown that Rubus idaeus can effectively prevent cancer invasion and reduce the rate of lung cancer growth in mice by inhibiting epithelial-to-mesenchymal transition (Hsieh et al, 2013;Chu et al, 2014). However, the mechanism by which Rubus idaeus inhibits the metastasis of NPC cells and the underlying mechanism remains unclear.…”

Section: Introductionmentioning

confidence: 97%

“…The chemical profile of RIE was analyzed by using high-pressure liquid chromatograms (HPLC)-mass spectrometer as described previously (Hsieh et al, 2013). The RIE was dissolved in dimethylsulfoxide (DMSO) for the subsequent experiments.…”

Section: Preparation Of Rubus Idaeus L Extracts (Rie)mentioning

confidence: 99%

“…The dried raspberry (Rubus idaeus L.) was purchased from the stores (Taichung, Taiwan), and dried raspberry (100 g) were extracted twice with 500 mL of 50% ethanol in distilled water as described previously (Hsieh et al, 2013, Huang et al, 2017. The chemical profile of RIE was analyzed by using high-pressure liquid chromatograms (HPLC)-mass spectrometer as described previously (Hsieh et al, 2013).…”

Section: Preparation Of Rubus Idaeus L Extracts (Rie)mentioning

confidence: 99%

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Rubus idaeusInhibits Migration and Invasion of Human Nasopharyngeal Carcinoma Cells by Suppression of MMP-2 through Modulation of the ERK1/2 Pathway

et al. 2017

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Nasopharyngeal carcinoma (NPC) is characterized by a high incidence of metastasis in the neck lymph nodes, resulting in a poor prognosis and posing challenges for treatment. In this study, we investigated the in vitro antimetastatic properties of Rubus idaeus extract (RIE) on human nasopharyngeal carcinoma cells. HONE-1, NPC-39 and NPC-BM cells were subjected to RIE treatment, and effects on the migration and invasion of tumor cells were analyzed. The results showed that RIE suppressed the migration and invasion of NPC cells. Gelatin zymography assay, Western blotting and real-time PCR showed that matrix metalloproteinases-2 (MMP-2) enzyme activity, protein expression and mRNA levels were down-regulated by RIE treatment. To identify the signaling pathway, mitogen-activated protein kinase proteins were examined, which showed that phosphorylation of ERK1/2 was inhibited after the treatment of RIE. In summary, our data showed that RIE inhibited the migration and invasion of NPC cells by suppressing the expression of MMP-2 by The American Journal of Chinese Medicine, Vol. 45, No. 7, 1557-1572© 2017 down-regulating the ERK1/2 signaling pathway, suggesting that Rubus idaeus may serve as chemotherapeutic and chemopreventive agent for NPC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Preparation Of Rubus Idaeus L Extracts (Rie)mentioning

confidence: 99%

Section: Preparation Of Rubus Idaeus L Extracts (Rie)mentioning

confidence: 99%

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Rubus idaeusInhibits Migration and Invasion of Human Nasopharyngeal Carcinoma Cells by Suppression of MMP-2 through Modulation of the ERK1/2 Pathway

et al. 2017

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Updated evidence on raspberries as functional foods: Anticancer bioactivity and therapeutic implications

Azzini,

Barnaba,

Mattera

et al. 2024

Food Frontiers

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Raspberries (RBs) (Rubus idaeus) are rich in bioactive compounds with promising anticancer properties. This review provides a comprehensive analysis of the anticancer effects of RBs, highlighting their potential as natural agents in cancer prevention and therapy. Bioactive compounds in RBs induce apoptosis, arrest the cell cycle, inhibit angiogenesis, and suppress metastasis. Preclinical studies demonstrate significant anticancer effects against colon, breast, lung, prostate, and cervical cancers, with clinical studies also supporting their therapeutic potential. Although preclinical findings are encouraging, further large‐scale clinical trials are needed to confirm their efficacy and safety in humans. Optimizing formulations to enhance bioavailability and therapeutic effectiveness is crucial. This review emphasizes the potential of dietary interventions involving RBs as a complementary approach to conventional cancer therapies, paving the way for future research and clinical innovations.

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Induction of apoptotic but not autophagic cell death by Cinnamomum cassia extracts on human oral cancer cells

Chu

Yang

et al. 2018

Journal Cellular Physiology

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Cinnamomum cassia has been widely studied in different fields to reveal its antidiabetic, antidepressive, antiviral, anti-inflammatory, antiosteoporotic, and anticancer effects. Its antimalignant activities have been explored in lung cancer, breast cancer, colorectal cancer, and even oral cancer, but the detailed signaling mechanism and effects of this plant on animal models need to be clarified. In the current study, C. cassia extract (CCE) was used to investigate the antitumorigenesis mechanism in vitro and in vivo. The major constituents of CCE used in this study were coumarin, cinnamic acid, and cinnamic aldehyde. CCE reduced the viability, number, and colony formation of human oral cancer cells, and induced their apoptosis. Caspase-3 activation, Bcl-2 reduction, and phosphatidylserine inversion were involved in CCE-stimulated apoptosis. CCE also enhanced the expression of autophagic markers, including acidic vesicular organelle, microtubule-associated protein 1 light chain 3-I, autophagy-related protein 14, rubicon, and p62. The combined treatment of CCE and caspase inhibitor significantly restored mitochondrial membrane potential (Δψ m ) and cell viability. However, the combined treatment of CCE and autophagy inhibitor further reduced the cell viability indicating that autophagy might be a survival pathway of CCE-treated SASVO3 cells. In contrast, CCE treatment for 12 days did not adversely affect SASVO3 tumor-bearing nude mice. CCE also elicited dosedependent effects on the decrease in tumor volume, tumor weight, and Ki-67 expression. These results suggested that CCE showed the potential for the complementary treatment of oral caner. K E Y W O R D S apoptosis, autophagy, Cinnamomum cassia, oral cancer

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Rubus idaeus L. reverses epithelial-to-mesenchymal transition and suppresses cell invasion and protease activities by targeting ERK1/2 and FAK pathways in human lung cancer cells

Cited by 49 publications

References 52 publications

Rubus idaeusInhibits Migration and Invasion of Human Nasopharyngeal Carcinoma Cells by Suppression of MMP-2 through Modulation of the ERK1/2 Pathway

Rubus idaeusInhibits Migration and Invasion of Human Nasopharyngeal Carcinoma Cells by Suppression of MMP-2 through Modulation of the ERK1/2 Pathway

Updated evidence on raspberries as functional foods: Anticancer bioactivity and therapeutic implications

Induction of apoptotic but not autophagic cell death by Cinnamomum cassia extracts on human oral cancer cells

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