Runx1 regulates osteogenic differentiation of BMSCs by inhibiting adipogenesis through Wnt/β-catenin pathway

Luo, Yuan; Zhang, Yingdi; Miao, Guojun; Zhang, Yiwen; Liu, Yuehua; Huang, Yuanliang

doi:10.1016/j.archoralbio.2018.10.028

Cited by 35 publications

(23 citation statements)

References 30 publications

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“…BMSCs are important stromal cells in osteogenesis and metabolism [34]. It has revealed that miR-149-3p and Runx1 can reduce the adipogenic differentiation of BMSCs and enhance the osteogenic differentiation [12,35]. In SANFH, the researchers found that BMSCs had specific differentiation to adipocytes, while the differentiation to osteoblasts was basically stagnant [14].…”

Section: Discussionmentioning

confidence: 99%

MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

Liu

Lei

2020

J Orthop Surg Res

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Background: It is reported that miR-596 has a potential diagnostic value for non-traumatic osteonecrosis of the femoral head (NOFH), but its underlying mechanisms in NOFH is unclear. Methods: The expression of miR-596 and Smad3 was detected by western blot and quantitative real-time PCR. The relationship between the two molecules was explored using Dual-Luciferase Reporter Assay. Glucocorticoid (GC)dexamethasone, was used to induce bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation, and the effects of miR-596 on BMSC osteogenic differentiation and proliferation were determined.Results: MiR-596 expression was upregulated, while Smad3 expression was inhibited in the bone marrow samples of patients with steroid-induced osteonecrosis of femoral head (SANFH). Overexpression of miR-596 inhibited the proliferation and osteogenic differentiation of BMSCs induced by GC. Meanwhile, the opposite results were observed in the miR-596 inhibitor group. In addition, Smad3 was a target gene of miR-596, and negatively regulated by miR-596. The promotion effect of the miR-596 inhibitor on BMSC proliferation and osteogenic differentiation was reversed by si-Smad3.Conclusion: MiR-596 can suppress GC-BMSC osteoblastic differentiation and proliferation by regulating Smad3 expression.

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Section: Discussionmentioning

confidence: 99%

MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

Liu

Lei

2020

J Orthop Surg Res

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“…Osteoblasts are the most important cells in the bone microenvironment, accounting for approximately 95% of the total cell population in bone tissues, and they can determine the proportion of components in bone tissues, whose main source is bone marrow mesenchymal stem cells (BMSCs) (19,20). Under certain conditions, BMSCs can differentiate into osteoblasts, adipocytes and chondrocytes, and there is a certain dynamic balance between osteogenic and adipogenic differentiation of BMSCs under normal conditions, which plays an important role during bone development (21)(22)(23).…”

Section: Discussionmentioning

confidence: 99%

miR‑205 regulates bone turnover in elderly female patients with type 2 diabetes mellitus through targeted inhibition of Runx2

Zhang

Zhao

et al. 2020

Exp Ther Med

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“…There is evidence that runX1 plays an important role in mediating the osteogenic differentiation of stem cells and this was associated with mir-128 (24). For example, luo et al (25) revealed that knockdown of runX1 in murine BM-MSCs significantly inhibited osteogenesis (as evaluated by lower alP activity and reduced calcium nodule formation) and decreased the expression levels of osteogenic-related genes (RUNX2, OCN and OPN) compared with…”

Section: Discussionmentioning

confidence: 99%

“…Further mechanism studies indicated that RUNX1 may exert its promoting roles in osteogenic differentiation by activating the canonical Wnt signaling pathway (including upregulation of β-catenin, lef1, Tcf1 and Wnt10b) (25). AXIN1 was previously considered as a negative regulator of the canonical Wnt/β-catenin signaling pathway via degradation of β-catenin with adenomatous polyposis coli (aPc), glycogen synthase kinase-3β (GSK-3β) and casein kinase 1 (28).…”

Section: Kmt2a Axin1 Adcy3 Pde8b Egfr Adcy6 Creb5mentioning

confidence: 99%

Mechanisms of miR‑128‑3p in inhibiting osteoblast differentiation from bone marrow‑derived mesenchymal stromal cells

et al. 2020

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The authors' previous study demonstrated that mir-128 may exert an inhibitory effect on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BM-MScs), but its downstream mechanisms remain to be elucidated. The aim of the present study was to investigate the microRNA (miRNA/miR) and mRNA profiles of differentiated and undifferentiated BM-MScs and explore new downstream targets for mir-128. The sequencing datasets of GSe107279 (mirna) and GSe112318 (mrna) were downloaded from the Gene expression omnibus database. The differentially expressed mirnas (deMs) and genes (deGs) were identified using the DESeq2 method. The target genes of deMs were predicted by the mirwalk 2.0 database. The hub target genes of mir-128 were screened by constructing the protein-protein interaction (PPi) network and module analysis. The expression levels of mir-128 and crucial target genes were validated by reverse transcription-quantitative (rT-q) Pcr before or after transfection of mir-128 mimics to BM-MSCs. The miRNA expression profile analysis identified miR-128 as one of the significantly downregulated DEMs (total 338) in differentiated BM-MScs compared with the undifferentiated control. a total of 103 predicted target genes of mir-128-3p were overlapped with upregulated deGs. By calculating the topological properties of each protein in the PPi network, 6 upregulated genes (KIT, NTRK2, YWHAB, GAB1, AXIN1 and RUNX1; fold change was the highest for NTRK2) were considered to be hub genes. of these, 4 were enriched in module 4 (RUNX1, KIT, GAB1 and AXIN1; RUNX1 was particularly crucial as it can interact with the others), while one was enriched in module 7 (YWHAB). The expression levels of mir-128 and these 6 target genes during the osteogenic differentiation were experimentally confirmed by rT-qPcr. in addition, the expression levels of these 6 genes were significantly reversed after transfection of miR-128-3p mimics into rat BM-MScs compared with the mir-control group. These findings indicated that miR-128-3p may inhibit the osteoblast differentiation of BM-MScs by downregulation of these 6 genes, particularly RUNX1, YWHAB and NTRK2.

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Runx1 regulates osteogenic differentiation of BMSCs by inhibiting adipogenesis through Wnt/β-catenin pathway

Cited by 35 publications

References 30 publications

MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

MiR-596 inhibits osteoblastic differentiation and cell proliferation by targeting Smad3 in steroid-induced osteonecrosis of femoral head

miR‑205 regulates bone turnover in elderly female patients with type 2 diabetes mellitus through targeted inhibition of Runx2

Mechanisms of miR‑128‑3p in inhibiting osteoblast differentiation from bone marrow‑derived mesenchymal stromal cells

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