Ruta Montana Evokes Antihypertensive Activity Through an Increase of Prostaglandins Release in L-NAME-Induced Hypertensive Rats

El-Ouady, Fadwa; Eddouks, Mohamed

doi:10.2174/1871530320666200628025430

Cited by 7 publications

(4 citation statements)

References 32 publications

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“…Indomethacin, a potent inhibitor of COX-1 and COX-2, inhibits the synthesis of prostaglandins. Previous literature reported that prostaglandins such as prostacyclin (PGI2) reduce blood pressure by increasing the level of cAMP leading to vasorelaxation and, ultimately, hypotension [ 8 , 9 ]. As in our study, indomethacin blocked the hypotensive effects of pulegone in rats, indicating that COX pathways may be involved in the hypotensive effect seen with pulegone.…”

Section: Discussionmentioning

confidence: 99%

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Pulegone Prevents Hypertension through Activation of Muscarinic Receptors and Cyclooxygenase Pathway in L-NAME-Induced Hypertensive Rats

Razzaq,

Younis,

Malik

et al. 2023

Cardiovascular Therapeutics

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The current study was designed to determine pulegone’s antihypertensive and vasoprotective activity in L-NAME-induced hypertensive rats. Firstly, the hypotensive dose-response relationship of pulegone was evaluated in normotensive anesthetized rats using the invasive method. Secondly, the mechanism involved in hypotensive activity was determined in the presence of pharmacological drugs such as atropine/muscarinic receptor blocker (1 mg/kg), L-NAME/NOS inhibitor (20 mg/kg), and indomethacin/COX inhibitor (5 mg/kg) in anesthetized rats. Furthermore, studies were carried out to assess the preventive effect of pulegone in L-NAME-induced hypertensive rats. Hypertension was induced in rats by administering L-NAME (40 mg/kg) orally for 28 days. Rats were divided into six groups which were treated orally with tween 80 (placebo), captopril (10 mg/kg), and different doses of pulegone (20 mg/kg, 40 mg/kg, and 80 mg/kg). Blood pressure, urine volume, sodium, and body weight were monitored weekly. After 28 days, the effect of pulegone on lipid profile, hepatic markers, antioxidant enzymes, and nitric oxide was estimated from the serum of treated rats. Moreover, plasma mRNA expression of eNOS, ACE, ICAM1, and EDN1 was measured using real-time PCR. Results show that pulegone dose-dependently decreased blood pressure and heart rate in normotensive rats, with the highest effect at 30 mg/kg/i.v. The hypotensive effect of pulegone was reduced in the presence of atropine and indomethacin, whereas L-NAME did not change its hypotensive effect. Concurrent treatment with pulegone for four weeks in L-NAME-treated rats caused a reduction in both systolic blood pressure and heart rate, reversed the reduced levels of serum nitric oxide (NO), and ameliorated lipid profile and oxidative stress markers. Treatment with pulegone also improved the vascular response to acetylcholine. Plasma mRNA expression of eNOS was reduced, whereas ACE, ICAM1, and EDN1 levels were high in the L-NAME group, which was facilitated by pulegone treatment. To conclude, pulegone prevented L-NAME-induced hypertension by demonstrating a hypotensive effect through muscarinic receptors and cyclooxygenase pathway, indicating its use as a potential candidate in managing hypertension.

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Section: Discussionmentioning

confidence: 99%

“…PGI2, primarily COX-2 derived, is a vasodilator expressed mainly by endothelial and vascular smooth muscle cells. It reduces blood pressure by increasing the level of cAMP, which leads to vasorelaxation [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Pulegone Prevents Hypertension through Activation of Muscarinic Receptors and Cyclooxygenase Pathway in L-NAME-Induced Hypertensive Rats

Razzaq,

Younis,

Malik

et al. 2023

Cardiovascular Therapeutics

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“…Moreover, it caused dose-dependent relaxation of the aorta. It is likely that the prostaglandin pathway mediates these processes [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Studies on the Accumulation of Secondary Metabolites and Evaluation of Biological Activity of In Vitro Cultures of Ruta montana L. in Temporary Immersion Bioreactors

Szewczyk

Marino

Taviano

et al. 2023

IJMS

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The present work focuses on in vitro cultures of Ruta montana L. in temporary immersion PlantformTM bioreactors. The main aim of the study was to evaluate the effects of cultivation time (5 and 6 weeks) and different concentrations (0.1–1.0 mg/L) of plant growth and development regulators (NAA and BAP) on the increase in biomass and the accumulation of secondary metabolites. Consequently, the antioxidant, antibacterial, and antibiofilm potentials of methanol extracts obtained from the in vitro-cultured biomass of R. montana were evaluated. High-performance liquid chromatography analysis was performed to characterize furanocoumarins, furoquinoline alkaloids, phenolic acids, and catechins. The major secondary metabolites in R. montana cultures were coumarins (maximum total content of 1824.3 mg/100 g DM), and the dominant compounds among them were xanthotoxin and bergapten. The maximum content of alkaloids was 561.7 mg/100 g DM. Concerning the antioxidant activity, the extract obtained from the biomass grown on the 0.1/0.1 LS medium variant, with an IC50 0.90 ± 0.03 mg/mL, showed the best chelating ability among the extracts, while the 0.1/0.1 and 0.5/1.0 LS media variants showed the best antibacterial (MIC range 125–500 µg/mL) and antibiofilm activity against resistant Staphylococcus aureus strains.

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“…Moreover, many in vitro and in vivo works have shown that RM, especially its EOs, exhibit various biological activities such as antibacterial (11,(16)(17)(18)(19)(20), antifungal (11,16,(20)(21)(22), antioxidant (11,16,(23)(24)(25), antidiabetic (26), anticancer (24), anti-fertility (27), antihypertensive (28), insecticidal, and larvicidal properties (29,30).…”

Section: Introductionmentioning

confidence: 99%

Ruta montana(L.) L.: An insight into its medicinal value, phytochemistry, biological properties, and toxicity

Benkhaira¹,

Koraichi²,

Fikri-Benbrahim³

2022

J Herbmed Pharmacol

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Ruta montana (RM) is a medicinal and aromatic plant (MAP) used in folk medicine, especially in North Africa, to treat digestive, infectious, respiratory, neurological, gynecological, and diabetic diseases. The current work aims to review the scientifically validated ethno-medicinal usage, bioactivities and phytochemistry of RM, in order to provide data support for further investigations. Data were procured from PubMed, Scopus, Google Scholar, Web of Science, ScienceDirect, and PubChem. The present study revealed that RM could be used to manage many diseases involved in public health problems, such as diabetes, hypertension, neurological disorders, infections, reproductive system disorders, and cancer. It might also replace chemical insecticides and fungicides since it exhibits antifungal, insecticidal, and larvicidal properties. RM extracts also contain mainly coumarins and alkaloids. The volatile oil of RM is characterized by an abundance of ketone compounds and 2-undecanone as major constituents. In the case of a high-dose administration, RM infusion can cause poisoning through the oral path. Thus, in-depth in vivo pharmacological studies and clinical trials are needed to transmute the traditional applications of RM into scientific-based information.

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Ruta Montana Evokes Antihypertensive Activity Through an Increase of Prostaglandins Release in L-NAME-Induced Hypertensive Rats

Cited by 7 publications

References 32 publications

Pulegone Prevents Hypertension through Activation of Muscarinic Receptors and Cyclooxygenase Pathway in L-NAME-Induced Hypertensive Rats

Pulegone Prevents Hypertension through Activation of Muscarinic Receptors and Cyclooxygenase Pathway in L-NAME-Induced Hypertensive Rats

Studies on the Accumulation of Secondary Metabolites and Evaluation of Biological Activity of In Vitro Cultures of Ruta montana L. in Temporary Immersion Bioreactors

Ruta montana(L.) L.: An insight into its medicinal value, phytochemistry, biological properties, and toxicity

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