Rutin has therapeutic effect on septic arthritis caused by Candida albicans

Ye, Han

doi:10.1016/j.intimp.2008.11.002

Cited by 79 publications

(57 citation statements)

References 34 publications

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“…Other authors also described the action of quercetin against Helicobacter pylori and Escherichia coli as well as synergistic activity with lecithin against Bacillus subtilis and the hepatitis A virus (Wu et al, 2008;Ramadan and Selim Asker, 2009). Rutin was shown to be effective against Bacillus cereus, Salmonella enteritidis and also in the treatment of arthritis caused by Candida albicans (Arima et al, 2002;Pereira et al, 2007;Han, 2009). The aim of this study was to evaluate the antifungal effect of the combination of amB with quercetin or rutin and the potential protective effect of quercetin and rutin on amB cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Quercetin and rutin as potential agents antifungal against Cryptococcus spp

et al. 2016

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Amphotericin B is a fungicidal substance that is treatment of choice for most systemic fungal infections affecting as cryptococcosis the immunocompromised patients. However, severe side effects have limited the utility of this drug. The aim of this study was to evaluate the antifungal effect of the combination of amphotericin B with quercetin or rutin and as a protective of citotoxic effect. The antifungal activity to amphotericin B, quercetin and rutin alone and in combination was determined in Candida sp and Cryptococcus neoformans strains. Cytotoxicity test on erythrocytes was performed by spectrophotometric absorbance of hemoglobin. The amphotericin B MIC was reduced when used in combination with quercetin or rutin to C. neoformans ATCC strain and reduced when combined with rutin to a clinical isolate of C. neoformans. In addition, the combination of quercetin with amphotericin B may reduce the toxicity of amphotericin B to red blood cells. Our results suggest that quercetin and rutin are potential agents to combine with amphotericin B in order to reduce the amphotericin dose to lessen side effects and improve antifungal efficacy.Keywords: amphotericin B, quercetin, rutin, Cryptococcus neoformans. Quercetina e rutina: potenciais agentes para terapia antifúngica ResumoA anfotericina B é uma substância fungicida e é o tratamento de escolha para a maioria das infecções fúngicas sistêmicas que afetam os pacientes imunocomprometidos, como a criptococose. No entanto, as severas reações adversas têm limitado a utilização desta droga. O objetivo deste estudo foi avaliar o efeito antifúngico e o potencial efeito protetor de citotoxicidade da combinação de anfotericina B com quercetina ou rutina. A atividade antifúngica de anfotericina B, quercetina e rutina, isoladamente e em combinação foi determinada em cepas de Candida sp e Cryptococcus neoformans. O teste de citotoxicidade em eritrócitos foi realizado por espectrofotometria, através da determinação da absorbância da hemoglobina. A concentração inibitória mínima da anfotericina B foi reduzida quando utilizada em combinação com a quercetina e rutina em C. neoformans ATCC e reduzida quando combinados com rutina em um isolado clínico de C. neoformans. Além disso, a combinação de quercetina com anfotericina B pode reduzir a toxicidade da droga em eritrócitos. Os resultados sugerem que quercetina e rutina são potenciais agentes para combinação com anfotericina B, a fim de reduzir a dose de anfotericina, diminuindo os efeitos colaterais e melhorando sua eficácia antifúngica.Palavras-chave: anfotericina B, quercetina, rutina, Cryptococcus neoformans.

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Section: Introductionmentioning

confidence: 99%

Quercetin and rutin as potential agents antifungal against Cryptococcus spp

et al. 2016

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“…The CACW contains the most antigenic sources responsible for pathogenesis of candidiasis (Han, 2005(Han, , 2009. The induction was induced as previously described (Han, 2005(Han, , 2009Lee et al, 2008). In brief, CACW (400 µg) dissolved in sterile DPBS was mixed with Complete Freund's Adjuvant (CFA; Difco) at a 1:1 ratio by volume.…”

Section: Induction Of Fungal Septic Arthritismentioning

confidence: 99%

“…Prior to these experiments, Och activity on mouse splenocytes stimulated with Con A was examined as previously described (Lee et al, 2008;Han, 2009). In addition, effect of Och on T cell activation, in the same experimental design as described above, was done with mouse splenocytes.…”

Section: Och Activity On T Cellsmentioning

confidence: 99%

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Involvement of T-cell immunoregulation by ochnaflavone in therapeutic effect on fungal arthritis due to Candida albicans

Lee

2011

Arch. Pharm. Res.

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Arthritis due to pathogenic fungi is a serious disease causing rapid destruction of the joint. In the pathogenesis of arthritis, T lymphocytes are considered to be one of the major immune cells. In present study, we examined the T cell immunoregulatory effect by ochnaflavone (Och), a biflavonoid, on arthritis caused by Candida albicans that is the most commonly associated with fungal arthritis. To examine the effects of ochnaflavonon Candida albicans-caused septic arthritis, an emulsified mixture of C. albicans cell wall and complete Freund's adjuvant (CACW/CFA) was injected into BALB/c mice via hind footpad route on days -3, -2, and -1. On Day 0, Och at 1 or 2 mg/dose/time was intratraperitoneally given to mice with the swollen footpad every other day for 3 times. The footpad-edema was measured for 20 days. Results revealed that Och reduced the edema at all dose levels and furthermore, there was app. 45% reduction of the edema in animals given 2 mg-dose at the peak of septic arthritis (p < 0.05). This anti-arthritic effect was accompanied by the diminishing of the DTH (delayed type hypersensitivity) activity against the CACW and by the provoking of the dominant T helper 2 (Th2) type cytokines production (IL-4 and Il-10), which appeared to result in a suppression of T helper 1 cytokines (IFN-γ and IL-2). Besides the T cell immunoregulatory activity, Och inhibited T cells activation as evidenced by the IL-2 reduction from PMA/ionomycin-stimulated Jurkat cell line and in addition, the compound killed macrophages in a dose-dependent manner (p < 0.05). However, Och caused no hemolysis (p < 0.05). These data implicate that Och, which has anti-arthritic activity based on the Th2 dominance as well as macrophage removal, can be safely administered into the blood circulation for treatment of the arthritis caused by C. albicans. Thus, it can be concluded that Och would be an ideal immunologically evaluated agent for treating of Candida arthritis.

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“…orange, grapefruit, lemon etc). It has several pharmacological activities including anti-inflammatory 1 , antioxidant 2 , neuroprotective 3 , cardioprotective 4 , antiarthritis 5 and psoriasis 6 . Rutin, a bioflavanoid has low solubility, less stability, low bioavailability and poor permeation 7 .…”

Section: Introductionmentioning

confidence: 99%