Rutin-Loaded Silver Nanoparticles With Antithrombotic Function

Abstract

In this paper, we fabricated rutin-loaded silver nanoparticles (Rutin@AgNPs) as the nano-anticoagulant with antithrombotic function. The serum stability, anticoagulation activity, and bleeding risk of Rutin@AgNPs were evaluated. The results showed Rutin@AgNPs had good serum stability, hemocompatibility, and cytocompatibility. The anticoagulation activity of rutin was maintained, and its stability and aqueous solubility were improved. The Rutin@AgNPs could provide a sustained release to prolong the half-life of rutin. The results of the coagulation parameter assay and thrombus formation test in mice model showed that the activated partial thromboplastin time and prothrombin time were prolonged, and Rutin@AgNPs inhibited the thrombosis in the 48 h period. Moreover, the limited bleeding time indicated that the Rutin@AgNPs significantly minimized the hemorrhage risk of rutin. This Rutin@AgNPs is a potential anticoagulant for antithrombotic therapy.

Rutin-Functionalized Multi-Walled Carbon Nanotubes: Molecular Docking, Physicochemistry and Cytotoxicity in Fibroblasts

“…The generation of free radicals and the pro-oxidant properties of natural agents appear to underlie their direct toxicity to tumor cells. More recently, rutin has been loaded in CNTs [ 12 ], maize starch nanoparticles [ 13 ], nanocrystals [ 14 ] and silver nanoparticles [ 15 ]. Rutin silver nanoparticles improved the stability and aqueous solubility of rutin and acted as a nano-anticoagulant with an antithrombotic function showing serum stability, hemocompatibility, and cytocompatibility [ 15 ].…”

“…More recently, rutin has been loaded in CNTs [ 12 ], maize starch nanoparticles [ 13 ], nanocrystals [ 14 ] and silver nanoparticles [ 15 ]. Rutin silver nanoparticles improved the stability and aqueous solubility of rutin and acted as a nano-anticoagulant with an antithrombotic function showing serum stability, hemocompatibility, and cytocompatibility [ 15 ]. Thus, the aim of this work was the functionalization of CNT with rutin, their physicochemical characterization, and the screening of their cytotoxicity profile.…”

Impact of Rutin and Other Phenolic Substances on the Digestibility of Buckwheat Grain Metabolites

“…Therefore, rutin-coated carbon nanotubes are one of the possibilities for delivering rutin to the cells [ 60 , 61 ]. Rutin-loaded nanoparticles of silver are also able to deliver rutin to the site of activity with antithrombotic function [ 62 ].…”

Enhanced Hemocompatibility of Silver Nanoparticles Using the Photocatalytic Properties of Titanium Dioxide

“…One is to construct a barrier layer to avoid direct contact between AgNPs and blood cells by utilizing biopolymer coatings or hydrogels ( Fischer et al, 2015 ; Marulasiddeshwara et al, 2017 ). The other category involves co-immobilizing AgNPs with anticoagulant biomolecules to enhance hemocompatibility ( Le Thi et al, 2017 ; Wu et al, 2020 ). However, both strategies are often cumbersome to prepare and do not address the hemocompatibility issue of AgNPs themselves.…”