Rutin (quercetin-3-rutinoside) modulates the hemostatic disturbances and redox imbalance induced by Bothrops jararaca snake venom in mice

Sachetto, Ana Teresa Azevedo; Rosa, Jaqueline Gomes; Santoro, Marcelo Larami

doi:10.1371/journal.pntd.0006774

Cited by 39 publications

(59 citation statements)

References 65 publications

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“…We found 29 papers that used this antibody to measure human, mouse, rabbit and canine TF by a variety of methods, including 15 that used the antibody for western blotting (Table S1). 33‐61 Importantly, Abcam evaluated the ability of the antibody to detect human TF in wild‐type and KO cell lines by western blotting 62 . Based on the data they obtained, they recently withdrew ab151748 and stated that “after evaluation, using a HAP1 knockout cell line, ab151748 was not shown to react specifically with the target protein and has therefore been discontinued.” We confirmed that this antibody does not detect mouse TF by western blotting but rather a nonspecific band.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting

Rosell

Moser

Hisada

et al. 2020

Research and Practice in Thrombosis and Haemostasis

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Background Western blotting is used to measure protein expression in cells and tissues. Appropriate interpretation of resulting data is contingent upon antibody validation. Objectives We assessed several commercial anti‐human and anti‐mouse tissue factor (TF) antibodies for their ability to detect TF by western blotting. Material and Methods We used human pancreatic cancer cell lines expressing different levels of TF and a mouse pancreatic cancer cell line expressing TF with a matched knockout derivative. Results Human and mouse TF protein detected by western blotting correlated with levels of TF mRNA in these cell lines. The apparent molecular weight of TF is increased by N‐linked glycosylation and, as expected, deglycosylation decreased the size of TF based on western blotting. We found that four commercial anti‐human TF antibodies detected TF in a TF‐positive cell line HPAF‐II whereas no signal was observed in a TF‐negative cell line MIA PaCa‐2. More variability was observed in detecting mouse TF. Two anti‐mouse TF antibodies detected mouse TF in a TF‐positive cell line and no signal was observed in a TF knockout cell line. However, a third anti‐mouse TF antibody detected a nonspecific protein in both the mouse TF‐positive and TF‐negative cell lines. Two anti‐human TF antibodies that are claimed to cross react with mouse TF either recognized a nonspecific band or did not detect mouse TF. Discussion Our results indicate that there is a range in quality of commercial anti‐TF antibodies. Conclusion We recommend that all commercial antibodies should be validated to ensure that they detect TF.

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Section: Discussionmentioning

confidence: 99%

Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting

Rosell

Moser

Hisada

et al. 2020

Research and Practice in Thrombosis and Haemostasis

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“…In Brazil, Bothrops jararaca snake venom (BjV) evokes hemostatic disturbances, bleeding manifestations, and redox status imbalance. Sachetto et al (2018) demonstrated the modulatory activity of rutin on the hematological, hemostatic and redox status markers altered by BjV injection in mice. It was found that rutin attenuated local hemorrhage, and the increase in reactive species, prevented the fall in RBC counts and fibrinogen levels, diminished tail bleeding and shortened prothrombin time evoked by envenomation.…”

Section: Haematoprotective Propertymentioning

confidence: 95%

A review on the chemistry and biological properties of Rutin, a promising nutraceutical agent

Prasad¹,

Prasad²

2019

Asian J Pharm Pharmacol

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Nature is an attractive source of new therapeutic agents and a variety of phytochemicals have proved to be very valuable in the prevention and treatment of various diseases. Many of the important drugs isolated from plants during the past 50 years have revolutionized modern medicinal practice (Dar et al., 2017). The use of herbal medicines and phytonutrients or nutraceuticals continues to expand rapidly across the world with many people now resorting to these products for the treatment of various health challenges in different national healthcare settings (WHO, 2004). It is estimated that up to 80% of the world's population living in the developing countries rely on herbal medicinal products as a primary source of healthcare and traditional medical practice as an integral part of the culture in their communities (Ekor, 2013). The medicinal compounds derived from various plants could be divided into three major biochemical classes: alkaloids (vinblastine, vincristine, pilocarpine, berberine, caffeine, piperine etc.), flavonoids (quercetin, kaempferol, chrysin, naringenin, genistein, rutin etc.) and terpenoids (artemisinin, taxol, digitoxin, azadarachtin, camphor, limonene etc.) (Takshak, 2018). These compounds have also gained importance in the area of nutraceuticals, which have positive health effects and may prove to be advantageous in the treatment of diseases like cancer, cardiovascular diseases and diabetes (Crozier et

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“…All procedures involving animals were in accordance with the National Guidelines from Conselho Nacional de Controle de Experimentação Animal (CONCEA) [34]. Blood and liver samples were collected from the same mice used previously [33].…”

Section: Ethics Statementmentioning

confidence: 99%

“…Mice from the saline control group were injected subcutaneously (s.c.) with sterile saline alone (vehicle), and mice from the BjV group were injected with freshly diluted BjV (1.6 mg/kg body weight, s.c.). The dose of BjV was based on a previous report [33], and it evoked characteristic hemostatic disturbances. After 3, 6 or 24 h, mice were anesthetized with isoflurane (induction and maintenance at 2.5%) for the collection of blood and tissues.…”

Section: Envenomationmentioning

confidence: 99%

“…We hypothesized that the expression of hemostasis-related genes was somehow stimulated after envenomation in the liver, the main organ involved in the synthesis of hemostatic factors, even in the absence of antivenom administration. To address this hypothesis, taking into account both the inflammatory and hemostatic events occurring during envenomation that may influence hepatic gene expression, we evaluated mRNA expression levels in livers of mice injected with BjV [33], a model that replicates the hemostatic manifestations observed in bitten patients.…”

Section: Introductionmentioning

confidence: 99%

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Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice

2020

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Few studies have addressed gene expression of hemostasis-related factors during acute thrombo-hemorrhagic diseases. Bites by the lanced-headed viper Bothrops jaracaca induce rapid hemostatic disturbances in victims, leading to systemic bleedings, thrombocytopenia and consumption coagulopathy. Although circulating levels of coagulation factors recover rapidly after administration of specific antivenom therapy, it is unclear if B. jararaca venom (BjV) upregulates the mRNA synthesis of hepatic hemostasis-related factors, or if the recovery occurs under basal conditions after the neutralization of venom components by antivenom. Thus, we aimed to investigate if BjV regulates gene expression of important hemostasis-related factors synthetized by the liver. On that account, Swiss mice were injected with saline or BjV (1.6 mg/kg b.w, s.c.), and after 3, 6 and 24 h blood samples and liver fragments were collected to analyze mRNA expression by real-time qPCR. Increased gene expression of fibrinogen chains, haptoglobin and STAT3 was observed during envenomation, particularly at 3 and 6 h. At 24h, mRNA levels of F10 were raised, while those of Serpinc1, Proc and Adamts13 were diminished. Surprisingly, F3 mRNA levels were steadily decreased at 3 h. Gene expression of Thpo, F7, F5 Tfpi, Mug1 was unaltered. mRNA levels of Vwf, P4hb, F8, F2, Plg, and Serpinf2 were minimally altered, but showed important associations with Nfkb1 gene expression. In conclusion, snakebite envenomation upregulates hepatic mRNA synthesis particularly of fibrinogen chains, and acute-phase markers. This response explains the fast recovery of fibrinogen levels after antivenom administration to patients bitten by B. jararaca snakes.

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Rutin (quercetin-3-rutinoside) modulates the hemostatic disturbances and redox imbalance induced by Bothrops jararaca snake venom in mice

Cited by 39 publications

References 65 publications

Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting

Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting

A review on the chemistry and biological properties of Rutin, a promising nutraceutical agent

Liver gene regulation of hemostasis-related factors is altered by experimental snake envenomation in mice

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