1975
DOI: 10.1042/bj1510067
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S-adenosylmethionine decarboxylase from baker's yeast

Abstract: 1. S-Adenosyl-L-methionine decarboxylase (S-adenosyl-L-methionine carboxy-lyase, EC 4.1.1.50) was purified more than 1100-fold from extracts of Saccharomyces cerevisiae by affinity chromatography on columns of Sepharose containing covalently bound methylglyoxal bis(guanylhydrazone) (1,1′[(methylethanediylidene)dinitrilo]diguanidine) [Pegg, (1974) Biochem J. 141, 581-583]. The final preparation appeared to be homogeneous on polyacrylamide-gel electrophoresis at pH 8.4. 2. S-Adenosylmethionine decarboxylase acti… Show more

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Cited by 56 publications
(1 citation statement)
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“…Some samples were also stored in YE medium without compound supplementation for 2 hr after rehydration. These concentrations were based on previous studies to ensure yeast viability (Avila, García-Trevijano, Lu, Corrales, & Mato, 2004;Eisenberg et al, 2009;Pösö, Sinervirta, & Jänne, 1975;Rodríguez-Porrata et al, 2011). The dry weight of cells was calculated after maintaining the cell pellets at 60°C for 2 days.…”
Section: Desiccation-rehydration Processmentioning
confidence: 99%
“…Some samples were also stored in YE medium without compound supplementation for 2 hr after rehydration. These concentrations were based on previous studies to ensure yeast viability (Avila, García-Trevijano, Lu, Corrales, & Mato, 2004;Eisenberg et al, 2009;Pösö, Sinervirta, & Jänne, 1975;Rodríguez-Porrata et al, 2011). The dry weight of cells was calculated after maintaining the cell pellets at 60°C for 2 days.…”
Section: Desiccation-rehydration Processmentioning
confidence: 99%