S Phase Progression Is Required for Transcriptional Activation of the β-Phaseolin Promoter

Chandrasekharan, Mahesh B.; Li, Guofu; Bishop, Kenneth J.; Hall, Timothy C.

doi:10.1074/jbc.m307787200

Cited by 14 publications

(18 citation statements)

References 51 publications

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“…The resulting construct, p3RY/-6435S-uidA-nos3 0 /pUC, was digested with HindIII, end-filled with Klenow DNA polymerase, digested with EcoRI and subsequently ligated to EcoRI-SmaI-digested pHM301K to obtain p3RY/-6435S-uidA-nos3 0 /HM301K. Construction of pXVE-HisSPvALF for estradiol-inducible expression of PvALF in plants was previously described (Chandrasekharan et al, 2003b). A 1.7 kb fragment of PvALF was PCR amplified using PvAlf/pET (Chandrasekharan et al, 2003b) as template to eliminate the B3 region (DB3) from amino acid 593-761 (GenBank gi|7451508) and to incorporate the restriction sites EcoRV (at the 5 0 end), a translational stop codon and a SalI site at the 3 0 end.…”

Section: Plasmids For Plant Transformationmentioning

confidence: 99%

Interaction of PvALF and VP1 B3 domains with the β-phaseolin promoter

Carranco¹,

Chandrasekharan

Townsend³

et al. 2004

Plant Mol Biol

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The phas promoter is potently transcribed during embryogenesis but in vegetative tissues it is completely silenced by a rotationally positioned nucleosome. Ectopic expression in leaves of PvALF, a seed-specific transcription factor belonging to the plant-exclusive B3 domain-containing VP1/ABI3 family, leads to chromatin remodeling of the phas promoter, permitting transcriptional activation by the growth regulator abscisic acid (ABA). Specific interaction with RY elements present in 40-42 bp oligonucleotide probes has been shown in vitro for Arabidopsis ABI3 and the isolated B3 domain of maize VP1. Here, both in vivo and in vitro approaches were used to show physical interaction of the B3 domain of VP1 or PvALF to RY elements in the native phas promoter. In electrophoretic mobility shift assays, small changes in B3 domain concentration differentiated between RY element-specific and sequence non-specific DNA binding. Increased affinity of the PvALF B3 domain to RY elements was observed in the presence of histones and other basic proteins, possibly reflecting the ability of this B3 factor to interact with the phas promoter in its nucleosomal configuration.

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Section: Plasmids For Plant Transformationmentioning

confidence: 99%

Interaction of PvALF and VP1 B3 domains with the β-phaseolin promoter

Carranco¹,

Chandrasekharan

Townsend³

et al. 2004

Plant Mol Biol

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“…The 2.3-kb ALF coding region without the ATG start codon was amplified by PCR from pXVE-HisS-ALF (Chandrasekharan et al, 2003b) to incorporate flanking BtsI and PacI restriction enzyme sites and cloned into a pGEM-T vector to yield pGEM-T/ALF. The ALF coding region was then released by BsmI and PacI digestion and fused 39 to the 3xHA sequence in BsmI-PacIdigested pGEM-T/3xHA vector to give pGEM-T/HA-ALF.…”

Section: Plasmid Constructionmentioning

confidence: 99%

Ordered Histone Modifications Are Associated with Transcriptional Poising and Activation of the phaseolin Promoter

Chandrasekharan

Hall

2005

Plant Cell

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The phaseolin (phas) promoter drives copious production of transcripts encoding the protein phaseolin during seed embryogenesis but is silent in vegetative tissues, in which a nucleosome is positioned over its three-phased TATA boxes. Transition from the inactive state in transgenic Arabidopsis thaliana leaves was accomplished by ectopic expression of the transcription factor Phaseolus vulgaris ABI3-like factor (ALF) and application of abscisic acid (ABA). Placement of hemagglutinin-tagged ALF expression under the control of an estradiol-inducible promoter permitted chromatin immunoprecipitation analysis of chronological changes in histone modifications, notably increased acetylation of H3-K9 and H4-K12, as phas chromatin was remodeled (potentiated). A different array of changes, including acetylation of H3-K14 and methylation of H3-K4, was found to be associated with ABA-mediated activation. Thus, temporal separation of phas potentiation from activation revealed that histone H3 and H4 Lys residues are not globally hyperacetylated during phas expression. Whereas decreases in histone H3 and H4 levels were detected during ALF-mediated remodeling, slight increases occurred after ABA-mediated activation, suggesting the restoration of histone-phas interactions or the replacement of histones in the phas chromatin. The observed histone modifications provide insight into factors involved in the euchromatinization and activation of a plant gene and expand the evidence for histone code conservation among eukaryotes.

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“…Through forward and reverse genetic studies, many genes involved in ABA responses have been isolated and characterized (Finkelstein et al, 2002;Koornneef et al, 2002;Xiong et al, 2002;Shinozaki and Yamaguchi-Shinozaki, 2007). Increased ABA in plant cells inhibits DNA replication and cell division, which results in retarded plant growth (Finkelstein et al, 2002;Swiatek et al, 2002;Chandrasekharan et al, 2003). However, the molecular mechanism for ABA inhibition of plant growth is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

et al. 2009

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Based on abscisic acid (ABA) inhibition of seed germination and seedling growth assays, we isolated an ABA overly sensitive mutant (abo4-1) caused by a mutation in the Arabidopsis thaliana POL2a/TILTED1(TIL1) gene encoding a catalytic subunit of DNA polymerase «. The dominant, ABA-insensitive abi1-1 or abi2-1 mutations suppressed the ABA hypersensitivity of the abo4-1 mutant. The abo4/til1 mutation reactivated the expression of the silenced Athila retrotransposon transcriptional silent information (TSI) and the silenced 35S-NPTII in the ros1 mutant and increased the frequency of somatic homologous recombination (HR) ;60-fold. ABA upregulated the expression of TSI and increased HR in both the wild type and abo4-1. MEIOTIC RECOMBINATION11 and GAMMA RESPONSE1, both of which are required for HR and double-strand DNA break repair, are expressed at higher levels in abo4-1 and are enhanced by ABA, while KU70 was suppressed by ABA. abo4-1 mutant plants are sensitive to UV-B and methyl methanesulfonate and show constitutive expression of the G2/Mspecific cyclin CycB1;1 in meristems. The abo4-1 plants were early flowering with lower expression of FLOWER LOCUS C and higher expression of FLOWER LOCUS T and changed histone modifications in the two loci. Our results suggest that ABO4/POL2a/TIL1 is involved in maintaining epigenetic states, HR, and ABA signaling in Arabidopsis.

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S Phase Progression Is Required for Transcriptional Activation of the β-Phaseolin Promoter

Cited by 14 publications

References 51 publications

Interaction of PvALF and VP1 B3 domains with the β-phaseolin promoter

Interaction of PvALF and VP1 B3 domains with the β-phaseolin promoter

Ordered Histone Modifications Are Associated with Transcriptional Poising and Activation of the phaseolin Promoter

Epigenetic Regulation, Somatic Homologous Recombination, and Abscisic Acid Signaling Are Influenced by DNA Polymerase ϵ Mutation inArabidopsis

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